ID   CFI2_ARATH              Reviewed;         223 AA.
AC   Q9FKW3; Q1PDF7;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Chalcone--flavanone isomerase 2;
DE            Short=Chalcone isomerase 2;
DE            EC=5.5.1.6;
GN   Name=CHI2; OrderedLocusNames=At5g66220; ORFNames=K2A18.30;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA   Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT   features of the regions of 1,381,565 bp covered by twenty one physically
RT   assigned P1 and TAC clones.";
RL   DNA Res. 5:131-145(1998).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=cv. Columbia;
RX   PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA   Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT   "Simultaneous high-throughput recombinational cloning of open reading
RT   frames in closed and open configurations.";
RL   Plant Biotechnol. J. 4:317-324(2006).
CC   -!- FUNCTION: Catalyzes the intramolecular cyclization of bicyclic
CC       chalcones into tricyclic (S)-flavanones. Responsible for the
CC       isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone)
CC       into naringenin (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a chalcone = a flavanone.; EC=5.5.1.6;
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q9FKW3-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9FKW3-2; Sequence=VSP_035545;
CC   -!- MISCELLANEOUS: Part of the biosynthetic pathway for all classes of
CC       flavonoids, a large class of secondary plant metabolites, many of which
CC       are brightly colored.
CC   -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice
CC       site. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the chalcone isomerase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB011474; BAB10427.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED98184.1; -; Genomic_DNA.
DR   EMBL; DQ447111; ABE66278.1; -; mRNA.
DR   RefSeq; NP_201423.2; NM_126020.2. [Q9FKW3-2]
DR   AlphaFoldDB; Q9FKW3; -.
DR   SMR; Q9FKW3; -.
DR   STRING; 3702.AT5G66230.2; -.
DR   PaxDb; Q9FKW3; -.
DR   PRIDE; Q9FKW3; -.
DR   EnsemblPlants; AT5G66220.1; AT5G66220.1; AT5G66220. [Q9FKW3-2]
DR   EnsemblPlants; AT5G66230.2; AT5G66230.2; AT5G66230.
DR   GeneID; 836754; -.
DR   Gramene; AT5G66220.1; AT5G66220.1; AT5G66220. [Q9FKW3-2]
DR   Gramene; AT5G66230.2; AT5G66230.2; AT5G66230.
DR   KEGG; ath:AT5G66220; -.
DR   Araport; AT5G66220; -.
DR   TAIR; locus:2156957; AT5G66220.
DR   eggNOG; ENOG502QR7K; Eukaryota.
DR   InParanoid; Q9FKW3; -.
DR   OMA; FVEIWKS; -.
DR   PhylomeDB; Q9FKW3; -.
DR   BioCyc; ARA:AT5G66220-MON; -.
DR   UniPathway; UPA00154; -.
DR   PRO; PR:Q9FKW3; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FKW3; baseline and differential.
DR   GO; GO:0045430; F:chalcone isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.890.20; -; 1.
DR   Gene3D; 3.50.70.10; -; 1.
DR   InterPro; IPR044164; CFI.
DR   InterPro; IPR016087; Chalcone_isomerase.
DR   InterPro; IPR016088; Chalcone_isomerase_3-sand.
DR   InterPro; IPR016089; Chalcone_isomerase_bundle_sf.
DR   InterPro; IPR036298; Chalcone_isomerase_sf.
DR   PANTHER; PTHR28039; PTHR28039; 1.
DR   Pfam; PF02431; Chalcone; 1.
DR   SUPFAM; SSF54626; SSF54626; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Flavonoid biosynthesis; Isomerase;
KW   Reference proteome.
FT   CHAIN           1..223
FT                   /note="Chalcone--flavanone isomerase 2"
FT                   /id="PRO_0000300831"
FT   BINDING         41
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         106
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         183
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   SITE            99
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000250"
FT   VAR_SEQ         1..49
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:17147637"
FT                   /id="VSP_035545"
SQ   SEQUENCE   223 AA;  24465 MW;  AD32AE0759F398FF CRC64;
     MPLPSVTPLH VDAFTFPPAV ESPASHKRLF LGGAGKFVIV TVIGVYLEAM ALPSISAKWK
     GKNAKELTES VPFFRQLVTG EFEKLARVTM KKRLTGIQYS EKVVENCEEI MKASGKYTRS
     EAKAIDQFLM VFKNQDFPPG SSIIFAICPK GSLTIAFSKE ERVPKTGKAV IKNKLLGEAV
     LESMIGKNGV SPATRKSLAE RLSKLMNKKD PYNEANVNVA TKN