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CFI2_CHRMO
ID   CFI2_CHRMO              Reviewed;         235 AA.
AC   A1E261;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   03-AUG-2022, entry version 46.
DE   RecName: Full=Chalcone--flavanone isomerase 2;
DE            Short=Chalcone isomerase 2;
DE            EC=5.5.1.6;
DE   AltName: Full=DgCHI2;
GN   Name=CHI2;
OS   Chrysanthemum morifolium (Florist's daisy) (Dendranthema grandiflorum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Asterales; Asteraceae; Asteroideae; Anthemideae;
OC   Artemisiinae; Chrysanthemum.
OX   NCBI_TaxID=41568;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Wembley; TISSUE=Petal;
RA   Huh E.J., Kim T.H., Cho K.H., Lee Y.R., Goo D.H., Lee K.S., Choi S.R.;
RT   "Color response of two red chrysanthemum cultivars to high temperature.";
RL   Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the intramolecular cyclization of bicyclic
CC       chalcones into tricyclic (S)-flavanones. Responsible for the
CC       isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone)
CC       into naringenin (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a chalcone = a flavanone.; EC=5.5.1.6;
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- MISCELLANEOUS: Part of the biosynthetic pathway for all classes of
CC       flavonoids, a large class of secondary plant metabolites, many of which
CC       are brightly colored.
CC   -!- SIMILARITY: Belongs to the chalcone isomerase family. {ECO:0000305}.
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DR   EMBL; EF094934; ABK88309.1; -; mRNA.
DR   AlphaFoldDB; A1E261; -.
DR   SMR; A1E261; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0045430; F:chalcone isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.890.20; -; 1.
DR   Gene3D; 3.50.70.10; -; 1.
DR   InterPro; IPR044164; CFI.
DR   InterPro; IPR016087; Chalcone_isomerase.
DR   InterPro; IPR016088; Chalcone_isomerase_3-sand.
DR   InterPro; IPR016089; Chalcone_isomerase_bundle_sf.
DR   InterPro; IPR036298; Chalcone_isomerase_sf.
DR   PANTHER; PTHR28039; PTHR28039; 1.
DR   Pfam; PF02431; Chalcone; 1.
DR   SUPFAM; SSF54626; SSF54626; 1.
PE   2: Evidence at transcript level;
KW   Flavonoid biosynthesis; Isomerase.
FT   CHAIN           1..235
FT                   /note="Chalcone--flavanone isomerase 2"
FT                   /id="PRO_0000314572"
FT   BINDING         50
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         192
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   SITE            108
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   235 AA;  25187 MW;  8EF891CE616646C2 CRC64;
     MATPSSATSL NVENIVFPSS VKPPGDTNTL FLGGAGVRGM EIQGNFVKFT GIGVYLEDKA
     IPLLAGKWKG KTAEELVNSV EFFRDIVTGP FKKFTQVTMI LPLTGKQYSE KVSEMCVGVW
     KAHGTYTDAD GATIDKFLEV FKDENFPPGA SILFTTSPDG SLTISFSKDG MIPEAANIVL
     ENEKLAQAVI ESVIGKNGVS PATKQSLASR LSDLMNHFDE KATTDAEPNL SKNGL
 
 
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