ID   CFI2_FRAAN              Reviewed;         237 AA.
AC   Q4AE12;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   03-AUG-2022, entry version 56.
DE   RecName: Full=Chalcone--flavanone isomerase 2;
DE            Short=Chalcone isomerase 2;
DE            EC=5.5.1.6;
GN   Name=CHI2; Synonyms=FrCHI17;
OS   Fragaria ananassa (Strawberry) (Fragaria chiloensis x Fragaria virginiana).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Rosales; Rosaceae; Rosoideae; Potentilleae; Fragariinae;
OC   Fragaria.
OX   NCBI_TaxID=3747;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Sachinoka; TISSUE=Fruit cortical tissue;
RA   Adachi Y., Chiba N., Suzuki G., Kano H., Nakamura S.;
RT   "Genes regulating anthocyanin-synthesis pathway in white wild strawberry.";
RL   Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the intramolecular cyclization of bicyclic
CC       chalcones into tricyclic (S)-flavanones. Responsible for the
CC       isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone)
CC       into naringenin (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a chalcone = a flavanone.; EC=5.5.1.6;
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- MISCELLANEOUS: Part of the biosynthetic pathway for all classes of
CC       flavonoids, a large class of secondary plant metabolites, many of which
CC       are brightly colored.
CC   -!- SIMILARITY: Belongs to the chalcone isomerase family. {ECO:0000305}.
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DR   EMBL; AB201754; BAE17120.1; -; mRNA.
DR   AlphaFoldDB; Q4AE12; -.
DR   SMR; Q4AE12; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0045430; F:chalcone isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.890.20; -; 1.
DR   Gene3D; 3.50.70.10; -; 1.
DR   InterPro; IPR044164; CFI.
DR   InterPro; IPR016087; Chalcone_isomerase.
DR   InterPro; IPR016088; Chalcone_isomerase_3-sand.
DR   InterPro; IPR016089; Chalcone_isomerase_bundle_sf.
DR   InterPro; IPR036298; Chalcone_isomerase_sf.
DR   PANTHER; PTHR28039; PTHR28039; 1.
DR   Pfam; PF02431; Chalcone; 1.
DR   SUPFAM; SSF54626; SSF54626; 1.
PE   2: Evidence at transcript level;
KW   Flavonoid biosynthesis; Isomerase.
FT   CHAIN           1..237
FT                   /note="Chalcone--flavanone isomerase 2"
FT                   /id="PRO_0000300837"
FT   BINDING         48
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         113
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         190
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   SITE            106
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   237 AA;  25468 MW;  F1E5838A3F189B34 CRC64;
     MAQSVTGIQI GGTSFPPAVK PPGSGNTLFL GGAGVRGMEI QGNFVKFTAI GVYLEDKAVP
     ALSVKWKGKT AEELTESVEF FREIVTGPFE KFTQVTMILP LTGQQYSEKV SENCVAIWKK
     FGIYTDAEAK AIEKFIEVFK DQTFPPGASI LFTQSPNGSL MIGISKDGSI PEVGNAVIEN
     KLLSESVLES IIGKQGVSPE ARKSVATRLS ELLKESDHCV AGNEKVDECT KEAENKA