ID   CFI2_MEDSA              Reviewed;         197 AA.
AC   P28013;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Chalcone--flavanone isomerase 2;
DE            Short=Chalcone isomerase 2;
DE            EC=5.5.1.6;
DE   Flags: Fragment;
GN   Name=CHI2; Synonyms=CHI-2;
OS   Medicago sativa (Alfalfa).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX   NCBI_TaxID=3879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Iroquois;
RX   PubMed=8193301; DOI=10.1007/bf00029858;
RA   McKhann H.I., Hirsch A.M.;
RT   "Isolation of chalcone synthase and chalcone isomerase cDNAs from alfalfa
RT   (Medicago sativa L.): highest transcript levels occur in young roots and
RT   root tips.";
RL   Plant Mol. Biol. 24:767-777(1994).
RN   [2]
RP   ERRATUM OF PUBMED:8193301.
RA   McKhann H.I., Hirsch A.M.;
RL   Plant Mol. Biol. 25:759-759(1994).
CC   -!- FUNCTION: Catalyzes the intramolecular cyclization of bicyclic
CC       chalcones into tricyclic (S)-flavanones. Responsible for the
CC       isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone)
CC       into naringenin (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a chalcone = a flavanone.; EC=5.5.1.6;
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- DEVELOPMENTAL STAGE: Highest expression in young root tips.
CC   -!- MISCELLANEOUS: Part of the biosynthetic pathway for all classes of
CC       flavonoids, a large class of secondary plant metabolites, many of which
CC       are brightly colored.
CC   -!- SIMILARITY: Belongs to the chalcone isomerase family. {ECO:0000305}.
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DR   EMBL; M91080; AAB41480.1; -; mRNA.
DR   AlphaFoldDB; P28013; -.
DR   SMR; P28013; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0045430; F:chalcone isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.890.20; -; 1.
DR   Gene3D; 3.50.70.10; -; 1.
DR   InterPro; IPR044164; CFI.
DR   InterPro; IPR016087; Chalcone_isomerase.
DR   InterPro; IPR016088; Chalcone_isomerase_3-sand.
DR   InterPro; IPR016089; Chalcone_isomerase_bundle_sf.
DR   InterPro; IPR036298; Chalcone_isomerase_sf.
DR   PANTHER; PTHR28039; PTHR28039; 1.
DR   Pfam; PF02431; Chalcone; 1.
DR   SUPFAM; SSF54626; SSF54626; 1.
PE   2: Evidence at transcript level;
KW   Flavonoid biosynthesis; Isomerase.
FT   CHAIN           <1..197
FT                   /note="Chalcone--flavanone isomerase 2"
FT                   /id="PRO_0000166435"
FT   BINDING         23
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         88
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         165
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   SITE            81
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
SQ   SEQUENCE   197 AA;  21309 MW;  FC95A6E6B18A19E2 CRC64;
     KSYFLGGAGE RGLTIEGNFI KFTAIGVYLE DIAVASLAAK WKGKSSEELL ETLDFYRDII
     SGPFEKLIRG SKIRELSGPE YSRKVMENCV AHLKSVGTYG DAEAEAMQKF AEAFKPVNFP
     PGASVFYRQS PDGILGLSFS PDTSIPEKEA ALIENKAVSS AVLETMIGEH AVSPDLKRCL
     AARLPALLNE GAFKIGN