ID   CFI2_VITVI              Reviewed;         234 AA.
AC   A5ANT9;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Chalcone--flavanone isomerase 2;
DE            Short=Chalcone isomerase 2;
DE            EC=5.5.1.6;
GN   Name=CHI2; ORFNames=VITISV_015868, VITISV_038602;
OS   Vitis vinifera (Grape).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; Vitales; Vitaceae; Viteae; Vitis.
OX   NCBI_TaxID=29760;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Pinot noir;
RX   DOI=10.1371/journal.pone.0001326;
RA   Velasco R., Zharkikh A., Troggio M., Cartwright D.A., Cestaro A., Pruss D.,
RA   Pindo M., FitzGerald L.M., Vezzulli S., Reid J., Malacarne G., Iliev D.,
RA   Coppola G., Wardell B., Micheletti D., Macalma T., Facci M., Mitchell J.T.,
RA   Perazzolli M., Eldredge G., Gatto P., Oyzerski R., Moretto M., Gutin N.,
RA   Stefanini M., Chen Y., Segala C., Davenport C., Dematte L., Mraz A.,
RA   Battilana J., Stormo K., Costa F., Tao Q., Si-Ammour A., Harkins T.,
RA   Lackey A., Perbost C., Taillon B., Stella A., Solovyev V., Fawcett J.A.,
RA   Sterck L., Vandepoele K., Grando S.M., Toppo S., Moser C., Lanchbury J.,
RA   Bogden R., Skolnick M., Sgaramella V., Bhatnagar S.K., Fontana P.,
RA   Gutin A., Van de Peer Y., Salamini F., Viola R.;
RT   "A high quality draft consensus sequence of the genome of a heterozygous
RT   grapevine variety.";
RL   PLoS ONE 2:E1326-E1326(2007).
CC   -!- FUNCTION: Catalyzes the intramolecular cyclization of bicyclic
CC       chalcones into tricyclic (S)-flavanones. Responsible for the
CC       isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone)
CC       into naringenin (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a chalcone = a flavanone.; EC=5.5.1.6;
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- MISCELLANEOUS: Part of the biosynthetic pathway for all classes of
CC       flavonoids, a large class of secondary plant metabolites, many of which
CC       are brightly colored.
CC   -!- SIMILARITY: Belongs to the chalcone isomerase family. {ECO:0000305}.
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DR   EMBL; AM430986; CAN60439.1; -; Genomic_DNA.
DR   EMBL; AM458653; CAN74526.1; -; Genomic_DNA.
DR   AlphaFoldDB; A5ANT9; -.
DR   SMR; A5ANT9; -.
DR   STRING; 29760.VIT_13s0067g03820.t01; -.
DR   PRIDE; A5ANT9; -.
DR   EnsemblPlants; Vitvi13g00225_t001; Vitvi13g00225_P001; Vitvi13g00225.
DR   Gramene; Vitvi13g00225_t001; Vitvi13g00225_P001; Vitvi13g00225.
DR   eggNOG; ENOG502QR5P; Eukaryota.
DR   HOGENOM; CLU_074682_0_0_1; -.
DR   OMA; WKGKTAQ; -.
DR   UniPathway; UPA00154; -.
DR   ExpressionAtlas; A5ANT9; baseline and differential.
DR   GO; GO:0045430; F:chalcone isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.890.20; -; 1.
DR   Gene3D; 3.50.70.10; -; 1.
DR   InterPro; IPR044164; CFI.
DR   InterPro; IPR016087; Chalcone_isomerase.
DR   InterPro; IPR016088; Chalcone_isomerase_3-sand.
DR   InterPro; IPR016089; Chalcone_isomerase_bundle_sf.
DR   InterPro; IPR036298; Chalcone_isomerase_sf.
DR   PANTHER; PTHR28039; PTHR28039; 1.
DR   Pfam; PF02431; Chalcone; 1.
DR   SUPFAM; SSF54626; SSF54626; 1.
PE   3: Inferred from homology;
KW   Flavonoid biosynthesis; Isomerase.
FT   CHAIN           1..234
FT                   /note="Chalcone--flavanone isomerase 2"
FT                   /id="PRO_0000300855"
FT   BINDING         50
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         115
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         192
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   SITE            108
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   234 AA;  25082 MW;  3AD4C60D7729316A CRC64;
     MSPVPSVTAV QVENVLFPPS VKPPGSTNDL FLGGAGVRGL EIQGKFVKFT AIGVYLESSA
     VPTLAVKWKG KTVEELADSV DFFRDVVTGP FEKFTKVTTI LPLTGRQYSD KVSENCVAFW
     KSVGIYTDAE AKAIEKFNEV LKDETFPPGN SILFTHSPLG ALTMSFSKDG SLPEVGNAVI
     ENKLLTEAVL ESIIGKHGVS PEAKKSLAAR LSELFCKEAG DEKIEAEKVA PVAC