ID   CFI_ARALP               Reviewed;         244 AA.
AC   Q9LKC3;
DT   26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Chalcone--flavanone isomerase;
DE            Short=Chalcone isomerase;
DE            EC=5.5.1.6;
GN   Name=CHI;
OS   Arabidopsis lyrata subsp. petraea (Northern rock-cress) (Cardaminopsis
OS   petraea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=59691;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Mjaellom;
RX   PubMed=10835405; DOI=10.1093/genetics/155.2.863;
RA   Kuittinen H., Aguade M.;
RT   "Nucleotide variation at the chalcone isomerase locus in Arabidopsis
RT   thaliana.";
RL   Genetics 155:863-872(2000).
CC   -!- FUNCTION: Catalyzes the intramolecular cyclization of bicyclic
CC       chalcones into tricyclic (S)-flavanones. Responsible for the
CC       isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone)
CC       into naringenin (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a chalcone = a flavanone.; EC=5.5.1.6;
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- MISCELLANEOUS: Part of the biosynthetic pathway for all classes of
CC       flavonoids, a large class of secondary plant metabolites, many of which
CC       are brightly colored.
CC   -!- SIMILARITY: Belongs to the chalcone isomerase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AJ287322; CAB94968.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9LKC3; -.
DR   SMR; Q9LKC3; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0045430; F:chalcone isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.890.20; -; 1.
DR   Gene3D; 3.50.70.10; -; 1.
DR   InterPro; IPR044164; CFI.
DR   InterPro; IPR016087; Chalcone_isomerase.
DR   InterPro; IPR016088; Chalcone_isomerase_3-sand.
DR   InterPro; IPR016089; Chalcone_isomerase_bundle_sf.
DR   InterPro; IPR036298; Chalcone_isomerase_sf.
DR   PANTHER; PTHR28039; PTHR28039; 1.
DR   Pfam; PF02431; Chalcone; 1.
DR   SUPFAM; SSF54626; SSF54626; 1.
PE   3: Inferred from homology;
KW   Flavonoid biosynthesis; Isomerase.
FT   CHAIN           1..244
FT                   /note="Chalcone--flavanone isomerase"
FT                   /id="PRO_0000166427"
FT   BINDING         57
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         122
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         199
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   SITE            115
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   244 AA;  26445 MW;  3AEBC15232B4B4E5 CRC64;
     MSFSPVSPSQ FPSVTKLQVD SVTFDPSVKS PASSNPLFLG GAGVRGLDIQ GKFVIFTVIG
     VYLESNAVPS LSVKWKGKTT EELSESVPFF REIVTGAFEK FIKVTMKLPL TGQQYSEKVT
     ENCVAIWKSL GIYTDCEAKA VEKFLEIFKE ETFPPGSSIL FALSPTGSLT VAFSRDDSIP
     ETGIAVIENK LLAEAVLESI IGKNGVSPGT RLSIAERLAK LMTKNKVEED ASNHSIDEKL
     AKEN