CFI_CANGE
ID CFI_CANGE Reviewed; 226 AA.
AC Q3Y4F4; Q3Y4F3;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2005, sequence version 1.
DT 03-AUG-2022, entry version 47.
DE RecName: Full=Chalcone--flavanone isomerase;
DE Short=Chalcone isomerase;
DE EC=5.5.1.6;
GN Name=CHI;
OS Canna generalis (Canna lily) (Canna glauca x Canna indica).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Zingiberales; Cannaceae; Canna.
OX NCBI_TaxID=341692;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RA Li J., Li M.R., Li H.Q.;
RT "Isolation and characterization of a Canna generalis gene encoding chalcone
RT flavonone isomerase.";
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the intramolecular cyclization of bicyclic
CC chalcones into tricyclic (S)-flavanones. Responsible for the
CC isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone)
CC into naringenin (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a chalcone = a flavanone.; EC=5.5.1.6;
CC -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC -!- MISCELLANEOUS: Part of the biosynthetic pathway for all classes of
CC flavonoids, a large class of secondary plant metabolites, many of which
CC are brightly colored.
CC -!- SIMILARITY: Belongs to the chalcone isomerase family. {ECO:0000305}.
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DR EMBL; DQ160231; AAZ80910.1; -; Genomic_DNA.
DR EMBL; DQ160232; AAZ80911.1; -; mRNA.
DR AlphaFoldDB; Q3Y4F4; -.
DR SMR; Q3Y4F4; -.
DR UniPathway; UPA00154; -.
DR GO; GO:0045430; F:chalcone isomerase activity; IEA:UniProtKB-EC.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.10.890.20; -; 1.
DR Gene3D; 3.50.70.10; -; 1.
DR InterPro; IPR044164; CFI.
DR InterPro; IPR016087; Chalcone_isomerase.
DR InterPro; IPR016088; Chalcone_isomerase_3-sand.
DR InterPro; IPR016089; Chalcone_isomerase_bundle_sf.
DR InterPro; IPR036298; Chalcone_isomerase_sf.
DR PANTHER; PTHR28039; PTHR28039; 1.
DR Pfam; PF02431; Chalcone; 1.
DR SUPFAM; SSF54626; SSF54626; 1.
PE 2: Evidence at transcript level;
KW Flavonoid biosynthesis; Isomerase.
FT CHAIN 1..226
FT /note="Chalcone--flavanone isomerase"
FT /id="PRO_0000314570"
FT BINDING 51
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 116
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 194
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT SITE 109
FT /note="Important for catalytic activity"
FT /evidence="ECO:0000250"
FT CONFLICT 66
FT /note="G -> A (in Ref. 1; AAZ80911)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 226 AA; 24228 MW; F954066B1C11D07B CRC64;
MAQAGVSLPK LVIGEVTFPP AAAPPGSSSS LFLAGAGERG LEIDRQFVVF TAIGVYLEDL
AVSTLGPKWK GKTADDLAGN SDFFRDIFTG PFEKFTRITM VKPLSGQQYS EKVEENCVAH
WKAAGTYTEA EAAAVEKFKE ACKNETFPPG TSILFTHQVS PASLTITFWR EGSMPETGNT
VIESKALSEA ILESIIGKHG VSPGAKRSVA QRLSEILEEV KLEGSK