ID   CFI_EUSER               Reviewed;         217 AA.
AC   Q6BEH3;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 1.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=Chalcone--flavanone isomerase;
DE            Short=Chalcone isomerase;
DE            EC=5.5.1.6;
GN   Name=CHI;
OS   Eustoma exaltatum subsp. russellianum (Bluebells) (Eustoma grandiflorum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Gentianales; Gentianaceae; Chironieae; Chironiinae;
OC   Eustoma.
OX   NCBI_TaxID=52518;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC   STRAIN=cv. Asuka no Sora; TISSUE=Petal;
RX   DOI=10.1111/j.1399-3054.2004.00407.x;
RA   Noda N., Kanno Y., Kato N., Kazuma K., Suzuki M.;
RT   "Regulation of gene expression involved in flavonol and anthocyanin
RT   biosynthesis during petal development in lisianthus (Eustoma
RT   grandiflorum).";
RL   Physiol. Plantarum 122:305-313(2004).
CC   -!- FUNCTION: Catalyzes the intramolecular cyclization of bicyclic
CC       chalcones into tricyclic (S)-flavanones. Responsible for the
CC       isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone)
CC       into naringenin (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a chalcone = a flavanone.; EC=5.5.1.6;
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- TISSUE SPECIFICITY: Petals. {ECO:0000269|Ref.1}.
CC   -!- MISCELLANEOUS: Part of the biosynthetic pathway for all classes of
CC       flavonoids, a large class of secondary plant metabolites, many of which
CC       are brightly colored.
CC   -!- SIMILARITY: Belongs to the chalcone isomerase family. {ECO:0000305}.
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DR   EMBL; AB078955; BAD34458.1; -; mRNA.
DR   AlphaFoldDB; Q6BEH3; -.
DR   SMR; Q6BEH3; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0045430; F:chalcone isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.890.20; -; 1.
DR   Gene3D; 3.50.70.10; -; 1.
DR   InterPro; IPR044164; CFI.
DR   InterPro; IPR016087; Chalcone_isomerase.
DR   InterPro; IPR016088; Chalcone_isomerase_3-sand.
DR   InterPro; IPR016089; Chalcone_isomerase_bundle_sf.
DR   InterPro; IPR036298; Chalcone_isomerase_sf.
DR   PANTHER; PTHR28039; PTHR28039; 1.
DR   Pfam; PF02431; Chalcone; 1.
DR   SUPFAM; SSF54626; SSF54626; 1.
PE   2: Evidence at transcript level;
KW   Flavonoid biosynthesis; Isomerase.
FT   CHAIN           1..217
FT                   /note="Chalcone--flavanone isomerase"
FT                   /id="PRO_0000300835"
FT   BINDING         50
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         115
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         191
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   SITE            108
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   217 AA;  23181 MW;  C3C16E764B746D50 CRC64;
     MAPPVSVSEV QVESYLFPPT ITPPASTKSF LLGGAGVRGL EIGGNFVKFT AIGIYLDETA
     IPWLAGKWKG KTAEELADSV DFFADIITGP FEKFTQVTLI LPLTGQQYSG KVSENCTAQW
     KAAGVYTPAD GKAIEKFLQV FEPQSFSPGD SILFMHSPES LTISFGKAGD IPETSNAVIE
     NKKLSEAVIE SVIGEKGVSP AAKKSLATRI AHVLNHF