ID   CFI_GENTR               Reviewed;         221 AA.
AC   Q565D8;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 53.
DE   RecName: Full=Chalcone--flavanone isomerase;
DE            Short=Chalcone isomerase;
DE            EC=5.5.1.6;
GN   Name=CHI;
OS   Gentiana triflora (Clustered gentian).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Gentianales; Gentianaceae; Gentianeae; Gentianinae;
OC   Gentiana.
OX   NCBI_TaxID=55190;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC   STRAIN=cv. Maciry; TISSUE=Petal;
RX   AGRICOLA=IND43694509; DOI=10.1016/j.plantsci.2005.01.009;
RA   Nakatsuka T., Nishihara M., Mishiba K., Yamamura S.;
RT   "Temporal expression of flavonoid biosynthesis-related genes regulates
RT   flower pigmentation in gentian plants.";
RL   Plant Sci. 168:1309-1318(2005).
CC   -!- FUNCTION: Catalyzes the intramolecular cyclization of bicyclic
CC       chalcones into tricyclic (S)-flavanones. Responsible for the
CC       isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone)
CC       into naringenin (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a chalcone = a flavanone.; EC=5.5.1.6;
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- TISSUE SPECIFICITY: Flowers. {ECO:0000269|Ref.1}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in flowers during the whole flower
CC       development. {ECO:0000269|Ref.1}.
CC   -!- MISCELLANEOUS: Part of the biosynthetic pathway for all classes of
CC       flavonoids, a large class of secondary plant metabolites, many of which
CC       are brightly colored.
CC   -!- SIMILARITY: Belongs to the chalcone isomerase family. {ECO:0000305}.
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DR   EMBL; D38168; BAD95484.1; -; mRNA.
DR   AlphaFoldDB; Q565D8; -.
DR   SMR; Q565D8; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0045430; F:chalcone isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.890.20; -; 1.
DR   Gene3D; 3.50.70.10; -; 1.
DR   InterPro; IPR044164; CFI.
DR   InterPro; IPR016087; Chalcone_isomerase.
DR   InterPro; IPR016088; Chalcone_isomerase_3-sand.
DR   InterPro; IPR016089; Chalcone_isomerase_bundle_sf.
DR   InterPro; IPR036298; Chalcone_isomerase_sf.
DR   PANTHER; PTHR28039; PTHR28039; 1.
DR   Pfam; PF02431; Chalcone; 1.
DR   SUPFAM; SSF54626; SSF54626; 1.
PE   2: Evidence at transcript level;
KW   Flavonoid biosynthesis; Isomerase.
FT   CHAIN           1..221
FT                   /note="Chalcone--flavanone isomerase"
FT                   /id="PRO_0000300838"
FT   BINDING         52
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         117
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         193
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   SITE            110
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   221 AA;  23544 MW;  C7C050212A90FE69 CRC64;
     MVSSSVSSVT EVKVESYVFP PSVKPPSSTK SFLLGGAGVR GLEINGNFVK FTAIGVYLEE
     SGVAVLSGKW KGKTAEELSD SVEFFTDIIT GPFEKFTQVT LILPVTGQQY SPKVAENCAA
     QWKAAGIYTD ADGIAIEKFL QVFQTESFTP GDSILFTHSP ESLTISFGKN GAIPEVSNAV
     IENKKLSEAV IESIIGEKGV SPAAKKSLAT RIAEILNHFD A