ID   CFI_GOSHI               Reviewed;         227 AA.
AC   A2IBF8;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   20-FEB-2007, sequence version 1.
DT   03-AUG-2022, entry version 56.
DE   RecName: Full=Chalcone--flavanone isomerase;
DE            Short=Chalcone isomerase;
DE            EC=5.5.1.6;
GN   Name=CHI;
OS   Gossypium hirsutum (Upland cotton) (Gossypium mexicanum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX   NCBI_TaxID=3635;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC   STRAIN=cv. T586; TISSUE=Fiber;
RX   PubMed=17482578; DOI=10.1016/j.bbrc.2007.04.084;
RA   Xiao Y.-H., Zhang Z.-S., Yin M.-H., Luo M., Li X.-B., Hou L., Pei Y.;
RT   "Cotton flavonoid structural genes related to the pigmentation in brown
RT   fibers.";
RL   Biochem. Biophys. Res. Commun. 358:73-78(2007).
CC   -!- FUNCTION: Catalyzes the intramolecular cyclization of bicyclic
CC       chalcones into tricyclic (S)-flavanones. Responsible for the
CC       isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone)
CC       into naringenin (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a chalcone = a flavanone.; EC=5.5.1.6;
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- TISSUE SPECIFICITY: Fibers. {ECO:0000269|PubMed:17482578}.
CC   -!- DEVELOPMENTAL STAGE: In young developing fibers.
CC       {ECO:0000269|PubMed:17482578}.
CC   -!- MISCELLANEOUS: Part of the biosynthetic pathway for all classes of
CC       flavonoids, a large class of secondary plant metabolites, many of which
CC       are brightly colored.
CC   -!- SIMILARITY: Belongs to the chalcone isomerase family. {ECO:0000305}.
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DR   EMBL; EF187439; ABM64798.1; -; mRNA.
DR   RefSeq; NP_001314370.1; NM_001327441.1.
DR   AlphaFoldDB; A2IBF8; -.
DR   SMR; A2IBF8; -.
DR   STRING; 3635.A2IBF8; -.
DR   GeneID; 107940815; -.
DR   KEGG; ghi:107940815; -.
DR   BRENDA; 5.5.1.6; 2499.
DR   UniPathway; UPA00154; -.
DR   Proteomes; UP000189702; Genome assembly.
DR   GO; GO:0005783; C:endoplasmic reticulum; ISA:AgBase.
DR   GO; GO:0042406; C:extrinsic component of endoplasmic reticulum membrane; ISA:AgBase.
DR   GO; GO:0005634; C:nucleus; ISA:AgBase.
DR   GO; GO:0009705; C:plant-type vacuole membrane; ISA:AgBase.
DR   GO; GO:0045430; F:chalcone isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; ISA:AgBase.
DR   GO; GO:0043473; P:pigmentation; IMP:AgBase.
DR   GO; GO:0009733; P:response to auxin; ISA:AgBase.
DR   GO; GO:0080167; P:response to karrikin; ISA:AgBase.
DR   GO; GO:0009411; P:response to UV; ISA:AgBase.
DR   GO; GO:0010224; P:response to UV-B; ISA:AgBase.
DR   Gene3D; 1.10.890.20; -; 1.
DR   Gene3D; 3.50.70.10; -; 1.
DR   InterPro; IPR044164; CFI.
DR   InterPro; IPR016087; Chalcone_isomerase.
DR   InterPro; IPR016088; Chalcone_isomerase_3-sand.
DR   InterPro; IPR016089; Chalcone_isomerase_bundle_sf.
DR   InterPro; IPR036298; Chalcone_isomerase_sf.
DR   PANTHER; PTHR28039; PTHR28039; 1.
DR   Pfam; PF02431; Chalcone; 1.
DR   SUPFAM; SSF54626; SSF54626; 1.
PE   2: Evidence at transcript level;
KW   Flavonoid biosynthesis; Isomerase; Reference proteome.
FT   CHAIN           1..227
FT                   /note="Chalcone--flavanone isomerase"
FT                   /id="PRO_0000300841"
FT   BINDING         50
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         115
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         192
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   SITE            108
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   227 AA;  24404 MW;  83A6B77E1FE97C0B CRC64;
     MSTSLSVTEL QVENFTFPPT VKPPGSTKTL FLGGAGERGL EIQGKFIKFT AIGVYLEDSA
     VNCLGVKWKG KSAVELTESV EFFRDVVTGD FEKFIRVTMI LPLTGQQYSE KVSENCVAIW
     KSLGIYTDAE AKAIEKFIEV FKDENFPPGS SILFTISGQG SLTIGFSKDS SVPEGGKVVI
     ENKLLANSVL ESVIGKNGVS PAAKESLASR LSPLFNDCGA DSEKPQS