ID   CFI_HORVU               Reviewed;         231 AA.
AC   Q8S3X0;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Chalcone--flavanone isomerase;
DE            Short=Chalcone isomerase;
DE            EC=5.5.1.6;
GN   Name=CHI;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND MUTAGENESIS OF GLY-32.
RC   STRAIN=cv. Morex;
RX   PubMed=12527208; DOI=10.1016/s0378-1119(02)01105-8;
RA   Druka A., Kudrna D., Rostoks N., Brueggeman R., von Wettstein D.,
RA   Kleinhofs A.;
RT   "Chalcone isomerase gene from rice (Oryza sativa) and barley (Hordeum
RT   vulgare): physical, genetic and mutation mapping.";
RL   Gene 302:171-178(2003).
CC   -!- FUNCTION: Catalyzes the intramolecular cyclization of bicyclic
CC       chalcones into tricyclic (S)-flavanones. Responsible for the
CC       isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone)
CC       into naringenin. {ECO:0000269|PubMed:12527208}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a chalcone = a flavanone.; EC=5.5.1.6;
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- MISCELLANEOUS: Part of the biosynthetic pathway for all classes of
CC       flavonoids, a large class of secondary plant metabolites, many of which
CC       are brightly colored.
CC   -!- SIMILARITY: Belongs to the chalcone isomerase family. {ECO:0000305}.
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DR   EMBL; AF474923; AAM13449.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8S3X0; -.
DR   SMR; Q8S3X0; -.
DR   EnsemblPlants; HORVU.MOREX.r2.5HG0437190.1; HORVU.MOREX.r2.5HG0437190.1; HORVU.MOREX.r2.5HG0437190.
DR   Gramene; HORVU.MOREX.r2.5HG0437190.1; HORVU.MOREX.r2.5HG0437190.1; HORVU.MOREX.r2.5HG0437190.
DR   OMA; WKGKTAQ; -.
DR   UniPathway; UPA00154; -.
DR   ExpressionAtlas; Q8S3X0; baseline and differential.
DR   GO; GO:0045430; F:chalcone isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.890.20; -; 1.
DR   Gene3D; 3.50.70.10; -; 1.
DR   InterPro; IPR044164; CFI.
DR   InterPro; IPR016087; Chalcone_isomerase.
DR   InterPro; IPR016088; Chalcone_isomerase_3-sand.
DR   InterPro; IPR016089; Chalcone_isomerase_bundle_sf.
DR   InterPro; IPR036298; Chalcone_isomerase_sf.
DR   PANTHER; PTHR28039; PTHR28039; 1.
DR   Pfam; PF02431; Chalcone; 1.
DR   SUPFAM; SSF54626; SSF54626; 1.
PE   1: Evidence at protein level;
KW   Flavonoid biosynthesis; Isomerase.
FT   CHAIN           1..231
FT                   /note="Chalcone--flavanone isomerase"
FT                   /id="PRO_0000300839"
FT   BINDING         46
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         112
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         189
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   SITE            105
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000250"
FT   MUTAGEN         32
FT                   /note="G->D: In ant30-245; loss of activity."
FT                   /evidence="ECO:0000269|PubMed:12527208"
SQ   SEQUENCE   231 AA;  23704 MW;  C1163ACAF769EA7B CRC64;
     MAVSELEVDG VVFPPLARPP GSAHAHFLAG AGVRGMEIGG HFIKFTAIGV YLQADAAVSA
     LAAKWAGKPA ADLASDAAFF RDVVTGEFEK FTRVTMILPL TGAQYSDKVT ENCVAYWKAA
     GVYTDAEAAA VDKFKEAFGP HSFAPGASIL FTHSPAGVLT VAFSKDSSVP ESGGVAIENA
     RLCEAVLESI IGEHGVSPAA KLSLANRVAE LLKGAAHAGG EPAAEPVPVS V