ID   CFI_ORYSJ               Reviewed;         233 AA.
AC   Q84T92; B7E6A0; Q8S3X1;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=Chalcone--flavanone isomerase;
DE            Short=Chalcone isomerase;
DE            EC=5.5.1.6;
GN   Name=CHI; Synonyms=CFI; OrderedLocusNames=Os03g0819600, LOC_Os03g60509;
GN   ORFNames=OJ1754_E06.14;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12527208; DOI=10.1016/s0378-1119(02)01105-8;
RA   Druka A., Kudrna D., Rostoks N., Brueggeman R., von Wettstein D.,
RA   Kleinhofs A.;
RT   "Chalcone isomerase gene from rice (Oryza sativa) and barley (Hordeum
RT   vulgare): physical, genetic and mutation mapping.";
RL   Gene 302:171-178(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16109971; DOI=10.1101/gr.3869505;
RG   The rice chromosome 3 sequencing consortium;
RA   Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA   Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA   Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA   Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA   Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA   Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA   Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA   Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA   Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA   Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA   O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA   Jin W., Lee H.R., Jiang J., Jackson S.;
RT   "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT   and diverged grass species.";
RL   Genome Res. 15:1284-1291(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- FUNCTION: Catalyzes the intramolecular cyclization of bicyclic
CC       chalcones into tricyclic (S)-flavanones. Responsible for the
CC       isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone)
CC       into naringenin (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a chalcone = a flavanone.; EC=5.5.1.6;
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- MISCELLANEOUS: Part of the biosynthetic pathway for all classes of
CC       flavonoids, a large class of secondary plant metabolites, many of which
CC       are brightly colored.
CC   -!- SIMILARITY: Belongs to the chalcone isomerase family. {ECO:0000305}.
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DR   EMBL; AF474922; AAM13448.1; -; Genomic_DNA.
DR   EMBL; AC104433; AAO65886.1; -; Genomic_DNA.
DR   EMBL; DP000009; ABF99575.1; -; Genomic_DNA.
DR   EMBL; AP008209; BAF13628.1; -; Genomic_DNA.
DR   EMBL; AP014959; BAS87077.1; -; Genomic_DNA.
DR   EMBL; AK061390; BAG87897.1; -; mRNA.
DR   EMBL; AK104791; BAG96953.1; -; mRNA.
DR   RefSeq; XP_015628287.1; XM_015772801.1.
DR   AlphaFoldDB; Q84T92; -.
DR   SMR; Q84T92; -.
DR   STRING; 4530.OS03T0819600-01; -.
DR   PaxDb; Q84T92; -.
DR   PRIDE; Q84T92; -.
DR   EnsemblPlants; Os03t0819600-01; Os03t0819600-01; Os03g0819600.
DR   EnsemblPlants; Os03t0819600-02; Os03t0819600-02; Os03g0819600.
DR   GeneID; 4334588; -.
DR   Gramene; Os03t0819600-01; Os03t0819600-01; Os03g0819600.
DR   Gramene; Os03t0819600-02; Os03t0819600-02; Os03g0819600.
DR   KEGG; osa:4334588; -.
DR   eggNOG; ENOG502QR5P; Eukaryota.
DR   HOGENOM; CLU_074682_0_0_1; -.
DR   InParanoid; Q84T92; -.
DR   OMA; WKGKTAQ; -.
DR   OrthoDB; 1158490at2759; -.
DR   PlantReactome; R-OSA-1119513; Pinobanksin biosynthesis.
DR   PlantReactome; R-OSA-1119531; Flavonoid biosynthesis.
DR   UniPathway; UPA00154; -.
DR   Proteomes; UP000000763; Chromosome 3.
DR   Proteomes; UP000059680; Chromosome 3.
DR   Genevisible; Q84T92; OS.
DR   GO; GO:0045430; F:chalcone isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.890.20; -; 1.
DR   Gene3D; 3.50.70.10; -; 1.
DR   InterPro; IPR044164; CFI.
DR   InterPro; IPR016087; Chalcone_isomerase.
DR   InterPro; IPR016088; Chalcone_isomerase_3-sand.
DR   InterPro; IPR016089; Chalcone_isomerase_bundle_sf.
DR   InterPro; IPR036298; Chalcone_isomerase_sf.
DR   PANTHER; PTHR28039; PTHR28039; 1.
DR   Pfam; PF02431; Chalcone; 1.
DR   SUPFAM; SSF54626; SSF54626; 1.
PE   2: Evidence at transcript level;
KW   Flavonoid biosynthesis; Isomerase; Reference proteome.
FT   CHAIN           1..233
FT                   /note="Chalcone--flavanone isomerase"
FT                   /id="PRO_0000300846"
FT   BINDING         47
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         113
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         192
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   SITE            106
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        135
FT                   /note="F -> L (in Ref. 1; AAM13448)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   233 AA;  23893 MW;  E12A5FA2002F95C8 CRC64;
     MAAVSEVEVD GVVFPPVARP PGSGHAHFLA GAGVRGVEIA GNFIKFTAIG VYLEEGAAVP
     ALAKKWAGKS ADELAADAAF FRDVVTGDFE KFTRVTMILP LTGEQYSDKV TENCVAAWKA
     AGVYTDAEGA AADKFKEAFK PHSFPPGASI LFTHSPPGVL TVAFSKDSSV PEGAVAAAAI
     ENRALCEAVL DSIIGEHGVS PAAKRSIAAR VSQLLKAEST GDVAAAEPAP VSA