ID   CFI_PHAVU               Reviewed;         221 AA.
AC   P14298; Q41117;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 2.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Chalcone--flavanone isomerase;
DE            Short=Chalcone isomerase;
DE            EC=5.5.1.6;
GN   Name=CHI;
OS   Phaseolus vulgaris (Kidney bean) (French bean).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Phaseolus.
OX   NCBI_TaxID=3885;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Tendergreen;
RA   Mehdy M.C., Zabelshansky M., Sathasivan K., Sheng J., Hsieh H.;
RT   "Structure of an elicitor-inducible chalcone isomerase gene in Phaseolus
RT   vulgaris.";
RL   Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 13-221.
RC   STRAIN=cv. Canadian Wonder;
RX   PubMed=1888895; DOI=10.1007/bf00017927;
RA   Blyden E.R., Derner P., Lamb C.J., Dixon R.A.;
RT   "Sequence analysis of a chalcone isomerase cDNA of Phaseolus vulgaris L.";
RL   Plant Mol. Biol. 16:167-169(1991).
CC   -!- FUNCTION: Catalyzes the intramolecular cyclization of bicyclic
CC       chalcones into tricyclic (S)-flavanones. Responsible for the
CC       isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone)
CC       into naringenin (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a chalcone = a flavanone.; EC=5.5.1.6;
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- MISCELLANEOUS: Part of the biosynthetic pathway for all classes of
CC       flavonoids, a large class of secondary plant metabolites, many of which
CC       are brightly colored.
CC   -!- SIMILARITY: Belongs to the chalcone isomerase family. {ECO:0000305}.
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DR   EMBL; Z15046; CAA78763.1; -; Genomic_DNA.
DR   EMBL; X16470; CAA34490.1; -; mRNA.
DR   PIR; S24784; S24784.
DR   RefSeq; XP_007142690.1; XM_007142628.1.
DR   AlphaFoldDB; P14298; -.
DR   SMR; P14298; -.
DR   STRING; 3885.XP_007142690.1; -.
DR   EnsemblPlants; ESW14684; ESW14684; PHAVU_007G008600g.
DR   GeneID; 18624493; -.
DR   Gramene; ESW14684; ESW14684; PHAVU_007G008600g.
DR   KEGG; pvu:PHAVU_007G008600g; -.
DR   eggNOG; ENOG502QR5P; Eukaryota.
DR   OMA; NCVAHLK; -.
DR   OrthoDB; 1158490at2759; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0045430; F:chalcone isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.890.20; -; 1.
DR   Gene3D; 3.50.70.10; -; 1.
DR   InterPro; IPR044164; CFI.
DR   InterPro; IPR016087; Chalcone_isomerase.
DR   InterPro; IPR016088; Chalcone_isomerase_3-sand.
DR   InterPro; IPR016089; Chalcone_isomerase_bundle_sf.
DR   InterPro; IPR036298; Chalcone_isomerase_sf.
DR   PANTHER; PTHR28039; PTHR28039; 1.
DR   Pfam; PF02431; Chalcone; 1.
DR   SUPFAM; SSF54626; SSF54626; 1.
PE   2: Evidence at transcript level;
KW   Flavonoid biosynthesis; Isomerase.
FT   CHAIN           1..221
FT                   /note="Chalcone--flavanone isomerase"
FT                   /id="PRO_0000166439"
FT   BINDING         50
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         115
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         192
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   SITE            108
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        212..221
FT                   /note="PAVLNGGIIV -> LRY (in Ref. 2; CAA34490)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   221 AA;  23686 MW;  B847FD285F166FF4 CRC64;
     MATAPTITDV QVEFLHFPAV VTSPATAKTY FLGGAGERGL TIEGKFIKFT AIGVYLEDKA
     VASLATKWKG KPSEELINTL DFYRDIISGP FEKLIRGSKI LQLSGTEYSR KVMENCVAHL
     KSVGTYGDAE AKGIEEFAEA FKKVNFPPGA SVFYRQSPDG ILGLSFSEDA TIPGEEAVVI
     ENKAVSAAVL ETMIGEHAVS PDLKRSLASR LPAVLNGGII V