ID   CFI_VERHY               Reviewed;         222 AA.
AC   Q2PF16;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 1.
DT   03-AUG-2022, entry version 52.
DE   RecName: Full=Chalcone--flavanone isomerase;
DE            Short=Chalcone isomerase;
DE            EC=5.5.1.6;
GN   Name=CHI;
OS   Verbena hybrida (Garden vervain) (Verbena hortensis).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Lamiales; Verbenaceae; Verbeneae; Glandularia.
OX   NCBI_TaxID=76714;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Temari Violet;
RA   Togami J., Tamura M., Ishiguro K., Hirose C., Okuhara H., Ueyama Y.,
RA   Nakamura N., Yonekura-Sakakibara K., Fukuchi-Mizutani M., Suzuki K.,
RA   Fukui Y., Kusumi T., Tanaka Y.;
RT   "Molecular characterization of the flavonoid biosynthesis of Verbena
RT   hybrida and the functional analysis of verbena and Clitoria ternatea F3'5'H
RT   genes in transgenic verbena.";
RL   Plant Biotechnol. 23:5-11(2006).
CC   -!- FUNCTION: Catalyzes the intramolecular cyclization of bicyclic
CC       chalcones into tricyclic (S)-flavanones. Responsible for the
CC       isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone)
CC       into naringenin (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a chalcone = a flavanone.; EC=5.5.1.6;
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- MISCELLANEOUS: Part of the biosynthetic pathway for all classes of
CC       flavonoids, a large class of secondary plant metabolites, many of which
CC       are brightly colored.
CC   -!- SIMILARITY: Belongs to the chalcone isomerase family. {ECO:0000305}.
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DR   EMBL; AB234907; BAE72880.1; -; mRNA.
DR   AlphaFoldDB; Q2PF16; -.
DR   SMR; Q2PF16; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0045430; F:chalcone isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.890.20; -; 1.
DR   Gene3D; 3.50.70.10; -; 1.
DR   InterPro; IPR044164; CFI.
DR   InterPro; IPR016087; Chalcone_isomerase.
DR   InterPro; IPR016088; Chalcone_isomerase_3-sand.
DR   InterPro; IPR016089; Chalcone_isomerase_bundle_sf.
DR   InterPro; IPR036298; Chalcone_isomerase_sf.
DR   PANTHER; PTHR28039; PTHR28039; 1.
DR   Pfam; PF02431; Chalcone; 1.
DR   SUPFAM; SSF54626; SSF54626; 1.
PE   2: Evidence at transcript level;
KW   Flavonoid biosynthesis; Isomerase.
FT   CHAIN           1..222
FT                   /note="Chalcone--flavanone isomerase"
FT                   /id="PRO_0000314575"
FT   BINDING         50
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         115
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         192
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   SITE            108
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   222 AA;  24020 MW;  A3ADBF59CAB8A46D CRC64;
     MIILPSITEV QVESVVFPPA VKPPGSDKTF FLGGAGVRGL EIEGKFIKFT AIGVYLENDA
     VTSLAAKWKG KTAEEVADSG DFFAEIVTGP FEKFTKVTTI LPLTGQQYSE KVVENCVAYW
     KAIGKFTDAE DEATKKFLQV FKNEMFHPGA SILFTQSPQG SLTISFSKDG SIPEKENAII
     ENKQLSEAVL ESIIGKKGVS PSAKQSLAAR LSDLLKQCES TT