CFM3A_ARATH
ID CFM3A_ARATH Reviewed; 881 AA.
AC F4J2U9; Q0WV92; Q9LS85;
DT 17-FEB-2016, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 73.
DE RecName: Full=CRM-domain containing factor CFM3A, chloroplastic/mitochondrial {ECO:0000305};
DE AltName: Full=Protein CRM FAMILY MEMBER 3A {ECO:0000303|PubMed:18799595};
DE Short=AtCFM3a {ECO:0000303|PubMed:18799595};
DE Flags: Precursor;
GN Name=CFM3A {ECO:0000303|PubMed:18799595};
GN OrderedLocusNames=At3g23070 {ECO:0000312|Araport:AT3G23070};
GN ORFNames=MXC7.10 {ECO:0000312|EMBL:BAB02098.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=18799595; DOI=10.1261/rna.1223708;
RA Asakura Y., Bayraktar O.A., Barkan A.;
RT "Two CRM protein subfamilies cooperate in the splicing of group IIB introns
RT in chloroplasts.";
RL RNA 14:2319-2332(2008).
CC -!- FUNCTION: Binds specific group II introns in chloroplasts and
CC facilitates their splicing. Acts on subgroup IIB introns. The
CC substrates of the subgroup IIB also require the CRM domain proteins
CC CAF1 or CAF2, with a simultaneous binding of CFM3A and CAF1 or CAF2.
CC Required for seed development. May influence the biogenesis of the
CC mitochondrial small ribosomal subunit. {ECO:0000269|PubMed:18799595}.
CC -!- SUBUNIT: Interacts with RNA. Part of large ribonucleo-protein particles
CC that contain CAF1 and/or CAF2, and RNC1.
CC {ECO:0000250|UniProtKB:A7XN92}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:18799595}. Mitochondrion
CC {ECO:0000269|PubMed:18799595}.
CC -!- DISRUPTION PHENOTYPE: Albino seeds that fail to germinate or yield very
CC slow-growing seedlings and stunted plants. The seeds of the double
CC mutant plants cfm3a-4 and cfm3b-2 fail to germinate.
CC {ECO:0000269|PubMed:18799595}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB02098.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAE98956.1; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
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DR EMBL; AB026655; BAB02098.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE76712.1; -; Genomic_DNA.
DR EMBL; AK226876; BAE98956.1; ALT_SEQ; mRNA.
DR RefSeq; NP_188947.2; NM_113207.4.
DR AlphaFoldDB; F4J2U9; -.
DR SMR; F4J2U9; -.
DR STRING; 3702.AT3G23070.1; -.
DR PaxDb; F4J2U9; -.
DR PRIDE; F4J2U9; -.
DR ProteomicsDB; 224460; -.
DR EnsemblPlants; AT3G23070.1; AT3G23070.1; AT3G23070.
DR GeneID; 821881; -.
DR Gramene; AT3G23070.1; AT3G23070.1; AT3G23070.
DR KEGG; ath:AT3G23070; -.
DR Araport; AT3G23070; -.
DR TAIR; locus:2094558; AT3G23070.
DR eggNOG; KOG1990; Eukaryota.
DR HOGENOM; CLU_006310_3_0_1; -.
DR InParanoid; F4J2U9; -.
DR OMA; GCNPLPV; -.
DR OrthoDB; 505933at2759; -.
DR PRO; PR:F4J2U9; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; F4J2U9; baseline and differential.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR GO; GO:0003729; F:mRNA binding; IEA:InterPro.
DR GO; GO:0000373; P:Group II intron splicing; IMP:TAIR.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0048316; P:seed development; IGI:TAIR.
DR Gene3D; 3.30.110.60; -; 3.
DR InterPro; IPR033262; CFM3.
DR InterPro; IPR045278; CRS1/CFM2/CFM3.
DR InterPro; IPR001890; RNA-binding_CRM.
DR InterPro; IPR035920; YhbY-like_sf.
DR PANTHER; PTHR31846; PTHR31846; 1.
DR PANTHER; PTHR31846:SF19; PTHR31846:SF19; 1.
DR Pfam; PF01985; CRS1_YhbY; 3.
DR SMART; SM01103; CRS1_YhbY; 3.
DR SUPFAM; SSF75471; SSF75471; 3.
DR PROSITE; PS51295; CRM; 3.
PE 2: Evidence at transcript level;
KW Chloroplast; Coiled coil; Mitochondrion; mRNA processing; mRNA splicing;
KW Plastid; Reference proteome; Repeat; Ribonucleoprotein; RNA-binding;
KW Transit peptide.
FT TRANSIT 1..50
FT /note="Chloroplast and mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 51..881
FT /note="CRM-domain containing factor CFM3A,
FT chloroplastic/mitochondrial"
FT /id="PRO_0000435532"
FT DOMAIN 219..315
FT /note="CRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00626"
FT DOMAIN 421..518
FT /note="CRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00626"
FT DOMAIN 636..736
FT /note="CRM 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00626"
FT REGION 799..856
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 588..617
FT /evidence="ECO:0000255"
FT COILED 752..786
FT /evidence="ECO:0000255"
FT COMPBIAS 802..833
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 881 AA; 100170 MW; 5CB03EC6B79B431C CRC64;
MAMKPSLHFC PTTVTKKFVY SFQSSFCFRF LRYSSSISLG SCKGVTFSSR NDQIASRRFS
FSRDCNNGVW LENWNRIQKR NQPKPPKVVV NYRKEGRFSG SEIVSGDDNR SRDGDGSTME
KIVEKLKKYG YMEEVQNKEI EQERRIEKGS VEDIFYVEEG KLPNTRGGFT EESLLGGENV
IGSNGDVGFP WEKMSAKEKK ELEAEWTAKK ENRYSLAEMT LPESELRRLR NLTFRTASKM
RIRGGGVTQV AVDAIKEKWK SAEIVRLKIE GASALNMRKM HEILEKKTGG LVIWRSGTSI
SLYRGVSYEL PSGKWNKQRR EETPPEAVIE NHDETTTMVD KSDEKVHLPQ LEQETTSVEK
KDQTSPVVEY EDELDELLDD LGPRFMDWPG DNPLPVDADL LPGAIPDYEP PFRVLPYGVR
SSLGPKEATA LRRLARSIPP HFALGRSRQL QGLATAMVRL WEKSMLAKIA IKRGVQSTTS
ERMAEDLKKL TGGIMLSRNK DFLVFYRGKN FLSREVADAL VEQERFVRTL QDEEEQARLR
GSSALIVPST EPANKLVSAG TLGETLDATG KWGKNLDDDD HSDEVKQEVE ILRHENLVRK
LERKLAFAER KLLKAERGLA KVEVCLKPAE QREDPESITD EERFMFRKLG LKMKAFLLLG
RRGVFDGTVE NMHLHWKYRE LVKIIVKAKT FDGVKKVALA LEAESGGILV SIDKVTKGYA
IIVYRGQDYK RPTMLRPKNL LTKRKALARS IELQRREGLL KHISTMQAKA KQLRAEIEQM
EKVTDKGDEE LYNKLDMAYA SSDEETDEED DDDAFPETYA EGEDGEEGEI LAGELSETED
KDLDSNESET GFGDDSVLFA EDLHTKSEDL PNKKVRLQHQ S
