CFR1_SCHPO
ID CFR1_SCHPO Reviewed; 620 AA.
AC Q92357;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Cell fusion protein cfr1;
DE AltName: Full=CHS5-related protein 1;
GN Name=cfr1; ORFNames=SPAC6G9.12;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=16598689; DOI=10.1002/yea.1361;
RA Cartagena-Lirola H., Duran A., Valdivieso M.-H.;
RT "The Schizosaccharomyces pombe cfr1(+) gene participates in mating through
RT a new pathway that is independent of fus1(+).";
RL Yeast 23:375-388(2006).
CC -!- FUNCTION: Required for cell fusion, independently of fus1. Appears to
CC have a role in transporting proteins that are involved in mating. May
CC act as a scaffold to retain cell fusion proteins in the cisternae of
CC the Golgi. Degraded at the onset of mating and this leads to release of
CC cell fusion proteins. {ECO:0000269|PubMed:16598689}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus {ECO:0000269|PubMed:16598689}.
CC -!- SIMILARITY: Belongs to the CHS5 family. {ECO:0000305}.
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DR EMBL; CU329670; CAB03614.1; -; Genomic_DNA.
DR PIR; T39074; T39074.
DR RefSeq; NP_594121.1; NM_001019545.2.
DR AlphaFoldDB; Q92357; -.
DR SMR; Q92357; -.
DR BioGRID; 278463; 17.
DR STRING; 4896.SPAC6G9.12.1; -.
DR iPTMnet; Q92357; -.
DR MaxQB; Q92357; -.
DR PaxDb; Q92357; -.
DR PRIDE; Q92357; -.
DR EnsemblFungi; SPAC6G9.12.1; SPAC6G9.12.1:pep; SPAC6G9.12.
DR GeneID; 2541978; -.
DR KEGG; spo:SPAC6G9.12; -.
DR PomBase; SPAC6G9.12; cfr1.
DR VEuPathDB; FungiDB:SPAC6G9.12; -.
DR eggNOG; ENOG502QRF7; Eukaryota.
DR HOGENOM; CLU_019904_3_0_1; -.
DR InParanoid; Q92357; -.
DR OMA; TPYEFQL; -.
DR PhylomeDB; Q92357; -.
DR PRO; PR:Q92357; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005769; C:early endosome; IDA:PomBase.
DR GO; GO:0034044; C:exomer complex; IDA:PomBase.
DR GO; GO:0005794; C:Golgi apparatus; IDA:PomBase.
DR GO; GO:0005802; C:trans-Golgi network; IDA:PomBase.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000747; P:conjugation with cellular fusion; IBA:GO_Central.
DR GO; GO:0006895; P:Golgi to endosome transport; IMP:PomBase.
DR GO; GO:0006893; P:Golgi to plasma membrane transport; ISO:PomBase.
DR GO; GO:0006896; P:Golgi to vacuole transport; IPI:PomBase.
DR CDD; cd13945; Chs5_N; 1.
DR CDD; cd00063; FN3; 1.
DR Gene3D; 2.60.40.10; -; 1.
DR Gene3D; 3.40.50.10190; -; 1.
DR InterPro; IPR001357; BRCT_dom.
DR InterPro; IPR036420; BRCT_dom_sf.
DR InterPro; IPR031673; Chs5_N.
DR InterPro; IPR031669; Fn3_2.
DR InterPro; IPR003961; FN3_dom.
DR InterPro; IPR036116; FN3_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR Pfam; PF16892; CHS5_N; 1.
DR Pfam; PF16893; fn3_2; 1.
DR Pfam; PF12738; PTCB-BRCT; 1.
DR SMART; SM00292; BRCT; 1.
DR SMART; SM00060; FN3; 1.
DR SUPFAM; SSF49265; SSF49265; 1.
DR SUPFAM; SSF52113; SSF52113; 1.
DR PROSITE; PS50172; BRCT; 1.
DR PROSITE; PS50853; FN3; 1.
PE 3: Inferred from homology;
KW Golgi apparatus; Reference proteome.
FT CHAIN 1..620
FT /note="Cell fusion protein cfr1"
FT /id="PRO_0000089660"
FT DOMAIN 79..169
FT /note="Fibronectin type-III"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT DOMAIN 167..256
FT /note="BRCT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00033"
FT REGION 287..566
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 588..620
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 287..328
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 336..371
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 372..392
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 393..431
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 447..470
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 492..516
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 522..540
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 620 AA; 67230 MW; 7782DD773F5577DA CRC64;
MDDTNQFMVS VAKIDAGMAI LLTPSFHIIE FPSVLLPNDA TAGSIIDISV HHNKEEEIAR
ETAFDDVQKE IFETYGQKLP SPPVLKLKNA TQTSIVLEWD PLQLSTARLK SLCLYRNNVR
VLNISNPMTT HNAKLSGLSL DTEYDFSLVL DTTAGTFPSK HITIKTLRMI DLTGIQVCVG
NMVPNEMEAL QKCIERIHAR PIQTSVRIDT THFICSSTGG PEYEKAKAAN IPILGLDYLL
KCESEGRLVN VSGFYIENRA SYNANASINS VEAAQNAAPN LNATTEQPKN TAEVAQGAAS
AKAPQQTTQQ GTQNSANAEP SSSASVPAEA PETEAEQSID VSSDIGLRSD SSKPNEAPTS
SENIKADQPE NSTKQENPEE DMQIKDAEEH SNLESTPAAQ QTSEVEANNH QEKPSSLPAV
EQINVNEENN TPETEGLEDE KEENNTAAES LINQEETTSG EAVTKSTVES SANEEEAEPN
EIIEENAVKS LLNQEGPATN EEVEKNNANS ENANGLTDEK IIEAPLDTKE NSDDDKPSPA
AAEDIGTNGA IEEIPQVSEV LEPEKAHTTN LQLNALDKEE DLNITTVKQS SEPTADDNLI
PNKEAEIIQS SDEFESVNID
