ID   CG121_ASHGO             Reviewed;         178 AA.
AC   Q75D21;
DT   06-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2013, sequence version 2.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=EKC/KEOPS complex subunit CGI121;
GN   Name=CGI121; OrderedLocusNames=ABR201W;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION, AND SEQUENCE REVISION TO C-TERMINUS.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Component of the EKC/KEOPS complex that is required for the
CC       formation of a threonylcarbamoyl group on adenosine at position 37
CC       (t(6)A37) in tRNAs that read codons beginning with adenine. The complex
CC       is probably involved in the transfer of the threonylcarbamoyl moiety of
CC       threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. CGI121 acts as
CC       an allosteric effector that regulates the t(6)A activity of the
CC       complex. The EKC/KEOPS complex also promotes both telomere uncapping
CC       and telomere elongation. The complex is required for efficient
CC       recruitment of transcriptional coactivators. CGI121 is not required for
CC       tRNA modification (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the EKC/KEOPS complex composed of at least BUD32,
CC       CGI121, GON7, KAE1 and PCC1; the whole complex dimerizes.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, telomere
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CGI121/TPRKB family. {ECO:0000305}.
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DR   EMBL; AE016815; AAS50974.2; -; Genomic_DNA.
DR   RefSeq; NP_983150.2; NM_208503.2.
DR   AlphaFoldDB; Q75D21; -.
DR   SMR; Q75D21; -.
DR   STRING; 33169.AAS50974; -.
DR   EnsemblFungi; AAS50974; AAS50974; AGOS_ABR201W.
DR   GeneID; 4619260; -.
DR   KEGG; ago:AGOS_ABR201W; -.
DR   eggNOG; KOG4066; Eukaryota.
DR   HOGENOM; CLU_065847_1_1_1; -.
DR   InParanoid; Q75D21; -.
DR   OMA; HNVHSEI; -.
DR   Proteomes; UP000000591; Chromosome II.
DR   GO; GO:0000781; C:chromosome, telomeric region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0000408; C:EKC/KEOPS complex; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:EnsemblFungi.
DR   GO; GO:0000722; P:telomere maintenance via recombination; IBA:GO_Central.
DR   GO; GO:0002949; P:tRNA threonylcarbamoyladenosine modification; IBA:GO_Central.
DR   Gene3D; 3.30.2380.10; -; 1.
DR   InterPro; IPR013926; CGI121/TPRKB.
DR   InterPro; IPR036504; CGI121/TPRKB_sf.
DR   PANTHER; PTHR15840; PTHR15840; 1.
DR   Pfam; PF08617; CGI-121; 1.
DR   SUPFAM; SSF143870; SSF143870; 1.
PE   3: Inferred from homology;
KW   Activator; Chromosome; Nucleus; Reference proteome; Telomere;
KW   Transcription; Transcription regulation; tRNA processing.
FT   CHAIN           1..178
FT                   /note="EKC/KEOPS complex subunit CGI121"
FT                   /id="PRO_0000279204"
SQ   SEQUENCE   178 AA;  20069 MW;  03066122986D2657 CRC64;
     MLTTTIPQFP DTKLYISLFR NVSNAPEIVD SLAELPYSII DARTLLSREQ LLSAVYRAML
     EKHCNKLRTR NLHTEVLLCL SPTSNVMSQI GDAMKRFGIK KDSTSVVLVK AVTGDETFDP
     KGFSEVIRGE ELEFSDTALR ESADIDTIRE NYKLKCFECE RLEELSAALV HAIQLRGL