CG121_CRYNB
ID CG121_CRYNB Reviewed; 229 AA.
AC P0CM65; Q55WU5; Q5KJF3;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 37.
DE RecName: Full=EKC/KEOPS complex subunit CGI121;
GN Name=CGI121; OrderedLocusNames=CNBC0450;
OS Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)
OS (Filobasidiella neoformans).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC Tremellales; Cryptococcaceae; Cryptococcus;
OC Cryptococcus neoformans species complex.
OX NCBI_TaxID=283643;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B-3501A;
RX PubMed=15653466; DOI=10.1126/science.1103773;
RA Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT neoformans.";
RL Science 307:1321-1324(2005).
CC -!- FUNCTION: Component of the EKC/KEOPS complex that is required for the
CC formation of a threonylcarbamoyl group on adenosine at position 37
CC (t(6)A37) in tRNAs that read codons beginning with adenine. The complex
CC is probably involved in the transfer of the threonylcarbamoyl moiety of
CC threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. CGI121 acts as
CC an allosteric effector that regulates the t(6)A activity of the
CC complex. The EKC/KEOPS complex also promotes both telomere uncapping
CC and telomere elongation. The complex is required for efficient
CC recruitment of transcriptional coactivators. CGI121 is not required for
CC tRNA modification (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the EKC/KEOPS complex composed of at least BUD32,
CC CGI121, GON7, KAE1 and PCC1; the whole complex dimerizes.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, telomere
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the CGI121/TPRKB family. {ECO:0000305}.
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DR EMBL; AAEY01000013; EAL21904.1; -; Genomic_DNA.
DR RefSeq; XP_776551.1; XM_771458.1.
DR AlphaFoldDB; P0CM65; -.
DR SMR; P0CM65; -.
DR EnsemblFungi; AAW42616; AAW42616; CNC06740.
DR EnsemblFungi; EAL21904; EAL21904; CNBC0450.
DR GeneID; 4934709; -.
DR KEGG; cnb:CNBC0450; -.
DR VEuPathDB; FungiDB:CNBC0450; -.
DR HOGENOM; CLU_065847_1_2_1; -.
DR Proteomes; UP000001435; Chromosome 3.
DR GO; GO:0000781; C:chromosome, telomeric region; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 3.30.2380.10; -; 1.
DR InterPro; IPR013926; CGI121/TPRKB.
DR InterPro; IPR036504; CGI121/TPRKB_sf.
DR PANTHER; PTHR15840; PTHR15840; 1.
DR Pfam; PF08617; CGI-121; 1.
DR SUPFAM; SSF143870; SSF143870; 1.
PE 3: Inferred from homology;
KW Activator; Chromosome; Nucleus; Telomere; Transcription;
KW Transcription regulation; tRNA processing.
FT CHAIN 1..229
FT /note="EKC/KEOPS complex subunit CGI121"
FT /id="PRO_0000410035"
FT REGION 87..120
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 100..115
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 229 AA; 25301 MW; 473F870D9955CF46 CRC64;
METYAYPAFP PEYSNIHIAL FKNVTNAPQI RKRLIEASQM TGPEGDKARE EVDFGFVEAN
LLVSKEHLLI AILSTLLYAF PSTGPAPTDP PPLSDIADPD VSSLSLSSSS ETRQPKTRSH
NLHSELLLLL SPNNNITDSI RRHGVSDITT NLAVVKFGKR GDRVEEVYEA MKNVVEGELI
GWEGISEGTD WARVDKIYKL NELNALKTAD MVEKKRTAVI STVAIKNVI