ID   CG121_CRYNJ             Reviewed;         229 AA.
AC   P0CM64; Q55WU5; Q5KJF3;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 42.
DE   RecName: Full=EKC/KEOPS complex subunit CGI121;
GN   Name=CGI121; OrderedLocusNames=CNC06740;
OS   Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC
OS   MYA-565) (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=214684;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JEC21 / ATCC MYA-565;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA   Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA   Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA   Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA   Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA   Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA   Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA   Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT   neoformans.";
RL   Science 307:1321-1324(2005).
CC   -!- FUNCTION: Component of the EKC/KEOPS complex that is required for the
CC       formation of a threonylcarbamoyl group on adenosine at position 37
CC       (t(6)A37) in tRNAs that read codons beginning with adenine. The complex
CC       is probably involved in the transfer of the threonylcarbamoyl moiety of
CC       threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. CGI121 acts as
CC       an allosteric effector that regulates the t(6)A activity of the
CC       complex. The EKC/KEOPS complex also promotes both telomere uncapping
CC       and telomere elongation. The complex is required for efficient
CC       recruitment of transcriptional coactivators. CGI121 is not required for
CC       tRNA modification (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the EKC/KEOPS complex composed of at least BUD32,
CC       CGI121, GON7, KAE1 and PCC1; the whole complex dimerizes.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, telomere
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CGI121/TPRKB family. {ECO:0000305}.
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DR   EMBL; AE017343; AAW42616.1; -; Genomic_DNA.
DR   RefSeq; XP_569923.1; XM_569923.1.
DR   AlphaFoldDB; P0CM64; -.
DR   SMR; P0CM64; -.
DR   STRING; 5207.AAW42616; -.
DR   PaxDb; P0CM64; -.
DR   EnsemblFungi; AAW42616; AAW42616; CNC06740.
DR   GeneID; 3256374; -.
DR   KEGG; cne:CNC06740; -.
DR   VEuPathDB; FungiDB:CNC06740; -.
DR   eggNOG; KOG4066; Eukaryota.
DR   HOGENOM; CLU_065847_1_2_1; -.
DR   InParanoid; P0CM64; -.
DR   OMA; RATTHIA; -.
DR   OrthoDB; 1360669at2759; -.
DR   Proteomes; UP000002149; Chromosome 3.
DR   GO; GO:0000781; C:chromosome, telomeric region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0000408; C:EKC/KEOPS complex; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0000722; P:telomere maintenance via recombination; IBA:GO_Central.
DR   GO; GO:0002949; P:tRNA threonylcarbamoyladenosine modification; IBA:GO_Central.
DR   Gene3D; 3.30.2380.10; -; 1.
DR   InterPro; IPR013926; CGI121/TPRKB.
DR   InterPro; IPR036504; CGI121/TPRKB_sf.
DR   PANTHER; PTHR15840; PTHR15840; 1.
DR   Pfam; PF08617; CGI-121; 1.
DR   SUPFAM; SSF143870; SSF143870; 1.
PE   3: Inferred from homology;
KW   Activator; Chromosome; Nucleus; Reference proteome; Telomere;
KW   Transcription; Transcription regulation; tRNA processing.
FT   CHAIN           1..229
FT                   /note="EKC/KEOPS complex subunit CGI121"
FT                   /id="PRO_0000279211"
FT   REGION          87..120
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        100..115
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   229 AA;  25301 MW;  473F870D9955CF46 CRC64;
     METYAYPAFP PEYSNIHIAL FKNVTNAPQI RKRLIEASQM TGPEGDKARE EVDFGFVEAN
     LLVSKEHLLI AILSTLLYAF PSTGPAPTDP PPLSDIADPD VSSLSLSSSS ETRQPKTRSH
     NLHSELLLLL SPNNNITDSI RRHGVSDITT NLAVVKFGKR GDRVEEVYEA MKNVVEGELI
     GWEGISEGTD WARVDKIYKL NELNALKTAD MVEKKRTAVI STVAIKNVI