ID   CG121_KLULA             Reviewed;         157 AA.
AC   Q6CT72;
DT   06-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=EKC/KEOPS complex subunit CGI121;
GN   Name=CGI121; OrderedLocusNames=KLLA0C14894g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Component of the EKC/KEOPS complex that is required for the
CC       formation of a threonylcarbamoyl group on adenosine at position 37
CC       (t(6)A37) in tRNAs that read codons beginning with adenine. The complex
CC       is probably involved in the transfer of the threonylcarbamoyl moiety of
CC       threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. CGI121 acts as
CC       an allosteric effector that regulates the t(6)A activity of the
CC       complex. The EKC/KEOPS complex also promotes both telomere uncapping
CC       and telomere elongation. The complex is required for efficient
CC       recruitment of transcriptional coactivators. CGI121 is not required for
CC       tRNA modification (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the EKC/KEOPS complex composed of at least BUD32,
CC       CGI121, GON7, KAE1 and PCC1; the whole complex dimerizes.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, telomere
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CGI121/TPRKB family. {ECO:0000305}.
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DR   EMBL; CR382123; CAH01718.1; -; Genomic_DNA.
DR   RefSeq; XP_452867.1; XM_452867.1.
DR   AlphaFoldDB; Q6CT72; -.
DR   SMR; Q6CT72; -.
DR   STRING; 28985.XP_452867.1; -.
DR   PRIDE; Q6CT72; -.
DR   EnsemblFungi; CAH01718; CAH01718; KLLA0_C14894g.
DR   GeneID; 2892689; -.
DR   KEGG; kla:KLLA0_C14894g; -.
DR   eggNOG; KOG4066; Eukaryota.
DR   HOGENOM; CLU_065847_1_1_1; -.
DR   InParanoid; Q6CT72; -.
DR   OMA; HNVHSEI; -.
DR   Proteomes; UP000000598; Chromosome C.
DR   GO; GO:0000781; C:chromosome, telomeric region; IEA:UniProtKB-SubCell.
DR   GO; GO:0000408; C:EKC/KEOPS complex; IEA:EnsemblFungi.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:EnsemblFungi.
DR   GO; GO:0000722; P:telomere maintenance via recombination; IEA:EnsemblFungi.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.2380.10; -; 1.
DR   InterPro; IPR013926; CGI121/TPRKB.
DR   InterPro; IPR036504; CGI121/TPRKB_sf.
DR   PANTHER; PTHR15840; PTHR15840; 1.
DR   Pfam; PF08617; CGI-121; 1.
DR   SUPFAM; SSF143870; SSF143870; 1.
PE   3: Inferred from homology;
KW   Activator; Chromosome; Nucleus; Reference proteome; Telomere;
KW   Transcription; Transcription regulation; tRNA processing.
FT   CHAIN           1..157
FT                   /note="EKC/KEOPS complex subunit CGI121"
FT                   /id="PRO_0000279214"
SQ   SEQUENCE   157 AA;  17527 MW;  394C9D47209C3564 CRC64;
     MAGIVLKYKI PQFQEYTIHV QKYNNVTNSS ELRSKLAELP FAYIDSKTVI SVEQLSSAIY
     KAILETEYNR MRTRTIHSEC ILSLSPSSNI GDALNRFGIK EGTTDIIVIK IIEDSNEEPY
     DGNASVDGDL VPVSDDEFST SADKEIIKTV CIPCDKL