ID   CG121_NEUCR             Reviewed;         207 AA.
AC   Q7SHG9;
DT   06-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=EKC/KEOPS complex subunit cgi121;
GN   Name=cgi121; ORFNames=NCU02935;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Component of the EKC/KEOPS complex that is required for the
CC       formation of a threonylcarbamoyl group on adenosine at position 37
CC       (t(6)A37) in tRNAs that read codons beginning with adenine. The complex
CC       is probably involved in the transfer of the threonylcarbamoyl moiety of
CC       threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Cgi121 acts as
CC       an allosteric effector that regulates the t(6)A activity of the
CC       complex. The EKC/KEOPS complex also promotes both telomere uncapping
CC       and telomere elongation. The complex is required for efficient
CC       recruitment of transcriptional coactivators. Cgi121 is not required for
CC       tRNA modification (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the EKC/KEOPS complex composed of at least bud32,
CC       cgi121, gon7, kae1 and pcc1; the whole complex dimerizes.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, telomere
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CGI121/TPRKB family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CM002236; EAA36381.1; -; Genomic_DNA.
DR   RefSeq; XP_965617.1; XM_960524.2.
DR   AlphaFoldDB; Q7SHG9; -.
DR   SMR; Q7SHG9; -.
DR   STRING; 5141.EFNCRP00000002289; -.
DR   EnsemblFungi; EAA36381; EAA36381; NCU02935.
DR   GeneID; 3881742; -.
DR   KEGG; ncr:NCU02935; -.
DR   VEuPathDB; FungiDB:NCU02935; -.
DR   HOGENOM; CLU_065847_1_0_1; -.
DR   InParanoid; Q7SHG9; -.
DR   OMA; HNVHSEI; -.
DR   Proteomes; UP000001805; Chromosome 1, Linkage Group I.
DR   GO; GO:0000781; C:chromosome, telomeric region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0000408; C:EKC/KEOPS complex; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0000722; P:telomere maintenance via recombination; IBA:GO_Central.
DR   GO; GO:0002949; P:tRNA threonylcarbamoyladenosine modification; IBA:GO_Central.
DR   Gene3D; 3.30.2380.10; -; 1.
DR   InterPro; IPR013926; CGI121/TPRKB.
DR   InterPro; IPR036504; CGI121/TPRKB_sf.
DR   PANTHER; PTHR15840; PTHR15840; 1.
DR   Pfam; PF08617; CGI-121; 1.
DR   SUPFAM; SSF143870; SSF143870; 1.
PE   3: Inferred from homology;
KW   Activator; Chromosome; Nucleus; Reference proteome; Telomere;
KW   Transcription; Transcription regulation; tRNA processing.
FT   CHAIN           1..207
FT                   /note="EKC/KEOPS complex subunit cgi121"
FT                   /id="PRO_0000279215"
SQ   SEQUENCE   207 AA;  23238 MW;  028BFC7FBC7AC4DC CRC64;
     MALETLQIEH VPSDHHVYAA LFRDVQNVDF LQAQLIGKNP EFEYAFIDAS VIISRTHLFA
     AIFRALNTLT EGTLQTPNVH SDIVIGLSPT NNISESYRRY GLTPSKTRDV LVIKIVYPKL
     SSENSTITSE QQPTPESIWQ HLTTHIQGTP VPLTNEELAK STDWAKVRKY YKLNGVPGVE
     RIDKADEEGR RKEMEGLVIS GIALRGL