ID   CG121_PYRAB             Reviewed;         171 AA.
AC   Q9UZ62;
DT   01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=KEOPS complex subunit Cgi121;
GN   Name=cgi121; OrderedLocusNames=PAB1522, PYRAB12920;
OS   Pyrococcus abyssi (strain GE5 / Orsay).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=272844;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GE5 / Orsay;
RX   PubMed=12622808; DOI=10.1046/j.1365-2958.2003.03381.x;
RA   Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O.,
RA   Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J.,
RA   Weissenbach J., Zivanovic Y., Forterre P.;
RT   "An integrated analysis of the genome of the hyperthermophilic archaeon
RT   Pyrococcus abyssi.";
RL   Mol. Microbiol. 47:1495-1512(2003).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=GE5 / Orsay;
RX   PubMed=22057919; DOI=10.1007/s00284-011-0035-x;
RA   Gao J., Wang J.;
RT   "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and
RT   Pyrococcus furiosus DSM 3638.";
RL   Curr. Microbiol. 64:118-129(2012).
RN   [3]
RP   FUNCTION IN T(6)A TRNA MODIFICATION, AND SUBUNIT.
RX   PubMed=23258706; DOI=10.1093/nar/gks1287;
RA   Perrochia L., Crozat E., Hecker A., Zhang W., Bareille J., Collinet B.,
RA   van Tilbeurgh H., Forterre P., Basta T.;
RT   "In vitro biosynthesis of a universal t6A tRNA modification in Archaea and
RT   Eukarya.";
RL   Nucleic Acids Res. 41:1953-1964(2013).
RN   [4]
RP   FUNCTION IN THE KEOPS COMPLEX, AND SUBUNIT.
RX   PubMed=23945934; DOI=10.1093/nar/gkt720;
RA   Perrochia L., Guetta D., Hecker A., Forterre P., Basta T.;
RT   "Functional assignment of KEOPS/EKC complex subunits in the biosynthesis of
RT   the universal t6A tRNA modification.";
RL   Nucleic Acids Res. 41:9484-9499(2013).
CC   -!- FUNCTION: Component of the KEOPS complex that is required for the
CC       formation of a threonylcarbamoyl group on adenosine at position 37
CC       (t(6)A37) in tRNAs that read codons beginning with adenine. The complex
CC       is probably involved in the transfer of the threonylcarbamoyl moiety of
CC       threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Cgi121 acts as
CC       an allosteric effector that regulates the t(6)A activity of the
CC       complex. Cgi121 is not required for tRNA modification.
CC       {ECO:0000269|PubMed:23258706, ECO:0000269|PubMed:23945934}.
CC   -!- SUBUNIT: Component of the KEOPS complex that consists of Kae1, Bud32,
CC       Cgi121 and Pcc1; the whole complex dimerizes.
CC       {ECO:0000269|PubMed:23258706, ECO:0000269|PubMed:23945934}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CGI121/TPRKB family. {ECO:0000305}.
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DR   EMBL; AJ248287; CAB50197.1; -; Genomic_DNA.
DR   EMBL; HE613800; CCE70731.1; -; Genomic_DNA.
DR   PIR; H75037; H75037.
DR   AlphaFoldDB; Q9UZ62; -.
DR   SMR; Q9UZ62; -.
DR   STRING; 272844.PAB1522; -.
DR   EnsemblBacteria; CAB50197; CAB50197; PAB1522.
DR   KEGG; pab:PAB1522; -.
DR   PATRIC; fig|272844.11.peg.1374; -.
DR   eggNOG; arCOG02197; Archaea.
DR   HOGENOM; CLU_1830686_0_0_2; -.
DR   OMA; GENFIVA; -.
DR   OrthoDB; 109859at2157; -.
DR   PhylomeDB; Q9UZ62; -.
DR   Proteomes; UP000000810; Chromosome.
DR   Proteomes; UP000009139; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0000408; C:EKC/KEOPS complex; IDA:UniProtKB.
DR   GO; GO:0050790; P:regulation of catalytic activity; IDA:UniProtKB.
DR   GO; GO:0002949; P:tRNA threonylcarbamoyladenosine modification; IDA:UniProtKB.
DR   Gene3D; 3.30.2380.10; -; 1.
DR   InterPro; IPR013926; CGI121/TPRKB.
DR   InterPro; IPR036504; CGI121/TPRKB_sf.
DR   Pfam; PF08617; CGI-121; 1.
DR   SUPFAM; SSF143870; SSF143870; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; tRNA processing.
FT   CHAIN           1..171
FT                   /note="KEOPS complex subunit Cgi121"
FT                   /id="PRO_0000430323"
SQ   SEQUENCE   171 AA;  19030 MW;  87117024527CA434 CRC64;
     MTPKFKNFKH DNSGQVETYL FEVISKYTML EIKTKFGKLC IAKVKLASES IERALNLCND
     NCQIVSAKCH EEVIFATILA IKAFSSGRNT AKTIKGEILL RLAGVKQIKD AIKIIGARKG
     ENLIIYFGVS DPCSSLKDFI SSLGLREEPL KPCDEDEVKR GFERAAIIEA L