CG12_YEAST
ID CG12_YEAST Reviewed; 545 AA.
AC P20438; D6W3B5; E9P938;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 2.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=G1/S-specific cyclin CLN2;
GN Name=CLN2; OrderedLocusNames=YPL256C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2569741; DOI=10.1073/pnas.86.16.6255;
RA Hadwiger J.A., Wittenberg C., Richardson H.E., de Barros Lopes M.,
RA Reed S.I.;
RT "A family of cyclin homologs that control the G1 phase in yeast.";
RL Proc. Natl. Acad. Sci. U.S.A. 86:6255-6259(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2197605; DOI=10.1093/nar/18.13.4025;
RA Hadwiger J.A., Reed S.I.;
RT "Nucleotide sequence of the Saccharomyces cerevisiae CLN1 and CLN2 genes.";
RL Nucleic Acids Res. 18:4025-4025(1990).
RN [3]
RP SEQUENCE REVISION.
RA Wittenberg C., Chapman-Shimshoni D.;
RL Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Essential for the control of the cell cycle at the G1/S
CC (start) transition. Interacts with the CDC28 protein kinase to form
CC MPF.
CC -!- INTERACTION:
CC P20438; P00546: CDC28; NbExp=10; IntAct=EBI-4483, EBI-4253;
CC -!- DEVELOPMENTAL STAGE: CLN1 and CLN2 mRNAs fluctuate periodically in the
CC cell cycle, peaking in G1 phase.
CC -!- MISCELLANEOUS: A dominant mutation in CLN2 gene (CLN2-1), advances the
CC G1- to S-phase transition in cycling cells and impairs the ability of
CC cells to arrest in G1 phase in response to external signals.
CC -!- MISCELLANEOUS: Present with 1270 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the cyclin family. {ECO:0000305}.
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DR EMBL; M33265; AAA65725.1; -; Genomic_DNA.
DR EMBL; Z73612; CAA97982.1; -; Genomic_DNA.
DR EMBL; AY693229; AAT93248.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11181.1; -; Genomic_DNA.
DR PIR; B33289; COBYC2.
DR RefSeq; NP_015067.1; NM_001184070.1.
DR AlphaFoldDB; P20438; -.
DR BioGRID; 35907; 357.
DR ComplexPortal; CPX-342; CLN2-CDC28 kinase complex.
DR DIP; DIP-2270N; -.
DR IntAct; P20438; 14.
DR MINT; P20438; -.
DR STRING; 4932.YPL256C; -.
DR iPTMnet; P20438; -.
DR PaxDb; P20438; -.
DR PRIDE; P20438; -.
DR EnsemblFungi; YPL256C_mRNA; YPL256C; YPL256C.
DR GeneID; 855819; -.
DR KEGG; sce:YPL256C; -.
DR SGD; S000006177; CLN2.
DR VEuPathDB; FungiDB:YPL256C; -.
DR eggNOG; KOG0653; Eukaryota.
DR GeneTree; ENSGT00940000176526; -.
DR HOGENOM; CLU_536434_0_0_1; -.
DR InParanoid; P20438; -.
DR OMA; ENCLMQY; -.
DR BioCyc; YEAST:G3O-34141-MON; -.
DR Reactome; R-SCE-3214858; RMTs methylate histone arginines.
DR Reactome; R-SCE-5687128; MAPK6/MAPK4 signaling.
DR Reactome; R-SCE-5689880; Ub-specific processing proteases.
DR Reactome; R-SCE-6804757; Regulation of TP53 Degradation.
DR Reactome; R-SCE-68949; Orc1 removal from chromatin.
DR Reactome; R-SCE-69017; CDK-mediated phosphorylation and removal of Cdc6.
DR Reactome; R-SCE-69202; Cyclin E associated events during G1/S transition.
DR Reactome; R-SCE-69231; Cyclin D associated events in G1.
DR Reactome; R-SCE-69656; Cyclin A:Cdk2-associated events at S phase entry.
DR Reactome; R-SCE-75815; Ubiquitin-dependent degradation of Cyclin D.
DR Reactome; R-SCE-9754119; Drug-mediated inhibition of CDK4/CDK6 activity.
DR Reactome; R-SCE-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR PRO; PR:P20438; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; P20438; protein.
DR GO; GO:0000307; C:cyclin-dependent protein kinase holoenzyme complex; IDA:SGD.
DR GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0016538; F:cyclin-dependent protein serine/threonine kinase regulator activity; IDA:SGD.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0044772; P:mitotic cell cycle phase transition; IBA:GO_Central.
DR GO; GO:0000321; P:re-entry into mitotic cell cycle after pheromone arrest; IGI:SGD.
DR GO; GO:1902806; P:regulation of cell cycle G1/S phase transition; IC:ComplexPortal.
DR GO; GO:0000079; P:regulation of cyclin-dependent protein serine/threonine kinase activity; IDA:SGD.
DR GO; GO:0007089; P:traversing start control point of mitotic cell cycle; IMP:SGD.
DR CDD; cd00043; CYCLIN; 1.
DR InterPro; IPR039361; Cyclin.
DR InterPro; IPR013763; Cyclin-like.
DR InterPro; IPR036915; Cyclin-like_sf.
DR InterPro; IPR014399; Cyclin_CLN.
DR InterPro; IPR006671; Cyclin_N.
DR PANTHER; PTHR10177; PTHR10177; 1.
DR Pfam; PF00134; Cyclin_N; 1.
DR PIRSF; PIRSF001770; Cyclin_CLN; 1.
DR SMART; SM00385; CYCLIN; 1.
DR SUPFAM; SSF47954; SSF47954; 1.
DR PROSITE; PS00292; CYCLINS; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Cyclin; Reference proteome.
FT CHAIN 1..545
FT /note="G1/S-specific cyclin CLN2"
FT /id="PRO_0000080412"
FT REGION 230..252
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 383..414
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 440
FT /note="Q -> R (in Ref. 6; AAT93248)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 545 AA; 61697 MW; D6426B94E040E960 CRC64;
MASAEPRPRM GLVINAKPDY YPIELSNAEL LSHFEMLQEY HQEISTNVIA QSCKFKPNPK
LIDQQPEMNP VETRSNIITF LFELSVVTRV TNGIFFHSVR LYDRYCSKRI VLRDQAKLVV
ATCLWLAAKT WGGCNHIINN VVIPTGGRFY GPNPRARIPR LSELVHYCGD GQVFDESMFL
QMERHILDTL NWNIYEPMIN DYVLNVDENC LMQYELYENQ VTYDKQCSEK RQSQLSQDSD
ATVDERPYQN EEEEEEDLKL KIKLINLKKF LIDVSAWQYD LLRYELFEVS HGIFSIINQF
TNQDHGPFLM TPMTSESKNG EILSTLMNGI VSIPNSLMEV YKTVNGVLPF INQVKEYHLD
LQRKLQIASN LNISRKLTIS TPSCSFENSN STSIPSPASS SQSHTPMRNM SSLSDNSVFS
RNMEQSSPIT PSMYQFGQQQ SNSICGSTVS VNSLVNTNNK QRIYEQITGP NSNNATNDYI
DLLNLNESNK ENQNPATAHY LNGGPPKTSF INHGMFPSPT GTINSGKSSS ASSLISFGMG
NTQVI
