CGEP_ARATH
ID CGEP_ARATH Reviewed; 960 AA.
AC Q8VZF3; O22913; Q8L635;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 03-MAY-2011, sequence version 2.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Probable glutamyl endopeptidase, chloroplastic;
DE EC=3.4.21.-;
DE Flags: Precursor;
GN Name=GEP; OrderedLocusNames=At2g47390; ORFNames=T8I13.23;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION.
RX DOI=10.1111/j.1399-3054.2005.00441.x;
RA Forsberg J., Stroem J., Kieselbach T., Larsson H., Alexciev K.,
RA Engstroem A., Aekerlund H.-E.;
RT "Protease activities in the chloroplast capable of cleaving an LHCII N-
RT terminal peptide.";
RL Physiol. Plantarum 123:21-29(2005).
CC -!- FUNCTION: Serine-type protease active in vitro against the LHCII N-
CC terminal. Cleaves its substrate on the carboxy-side of Glu residues (By
CC similarity). {ECO:0000250, ECO:0000269|Ref.4}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8VZF3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8VZF3-2; Sequence=VSP_039719;
CC -!- SIMILARITY: Belongs to the peptidase S9D family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB63841.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC002337; AAB63841.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC10835.1; -; Genomic_DNA.
DR EMBL; AY064997; AAL57645.1; -; mRNA.
DR EMBL; AY099560; AAM20412.1; -; mRNA.
DR EMBL; BT002650; AAO11566.1; -; mRNA.
DR PIR; F84914; F84914.
DR RefSeq; NP_850473.1; NM_180142.2. [Q8VZF3-2]
DR AlphaFoldDB; Q8VZF3; -.
DR BioGRID; 4687; 4.
DR STRING; 3702.AT2G47390.1; -.
DR ESTHER; arath-CGEP; Glutamyl_Peptidase_S9.
DR MEROPS; S09.021; -.
DR iPTMnet; Q8VZF3; -.
DR PaxDb; Q8VZF3; -.
DR PeptideAtlas; Q8VZF3; -.
DR PRIDE; Q8VZF3; -.
DR ProteomicsDB; 224477; -. [Q8VZF3-1]
DR EnsemblPlants; AT2G47390.1; AT2G47390.1; AT2G47390. [Q8VZF3-2]
DR GeneID; 819352; -.
DR Gramene; AT2G47390.1; AT2G47390.1; AT2G47390. [Q8VZF3-2]
DR KEGG; ath:AT2G47390; -.
DR Araport; AT2G47390; -.
DR TAIR; locus:2065200; AT2G47390.
DR eggNOG; KOG2100; Eukaryota.
DR HOGENOM; CLU_017120_0_0_1; -.
DR InParanoid; Q8VZF3; -.
DR OMA; FPIQSER; -.
DR OrthoDB; 265965at2759; -.
DR PRO; PR:Q8VZF3; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8VZF3; baseline and differential.
DR Genevisible; Q8VZF3; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009570; C:chloroplast stroma; IDA:TAIR.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0043621; F:protein self-association; IPI:TAIR.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IDA:TAIR.
DR GO; GO:0006508; P:proteolysis; IDA:TAIR.
DR Gene3D; 2.120.10.30; -; 1.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR011042; 6-blade_b-propeller_TolB-like.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR001375; Peptidase_S9.
DR Pfam; PF00326; Peptidase_S9; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chloroplast; Hydrolase; Plastid; Protease;
KW Reference proteome; Serine protease; Transit peptide.
FT TRANSIT 1..62
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 63..960
FT /note="Probable glutamyl endopeptidase, chloroplastic"
FT /id="PRO_0000397884"
FT REGION 78..98
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 915..960
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 82..98
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 780
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 854
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 888
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT VAR_SEQ 428
FT /note="L -> LY (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_039719"
FT CONFLICT 685
FT /note="C -> Y (in Ref. 3; AAL57645/AAM20412/AAO11566)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 960 AA; 106103 MW; C35EBC0B3B1DDF77 CRC64;
MMRFHKACHR FSLSPLCHLS PPSPSPASSL LLLPKLSGFS TLSTRRCVRV RRFSENPLTT
VMASRSASRL RSLASACSGG AEDGGGTSNG SLSASATATE DDELAIGTGY RLPPPEIRDI
VDAPPVPALS FSPHRDKILF LKRRALPPLA DLARPEEKLA GVRIDGYCNT RSRMSFYTGL
GIHQLLPDGT LSPEKEITGI PDGGKINFVT WSNDGKHLAF SIRVDENGNS SKPVVWVADV
ETGVARPLFN SQDIFLNAIF ESFVWIDNST LLVSTIPSSR GEPPKKPLVP SGPKTLSNET
KTVVQVRTFQ DLLKDEYDAD LFDYYASSQL VLASLDGTVK EVGVPAVYTS LDPSTDHKYL
LVSSLHRPYS FIVPCGRFPK KVEVWTTDGR FVRQLCDLPL AEDIPIASNS VRKGMRSINW
RADKPSTLWA ETQDGGDAKM EVSPRDIVYM QSAEPLAGEE PEVLHKLDLR YGGISWCDDT
LALVYESWYK TRRTRTWVIS PGSNDVSPRI LFDRSSEDVY SDPGSTMLRR TDAGTYVIAK
IKKENDEGTY VLLNGSGATP QGNVPFLDLF DINTGNKERI WESDKEKYFE TVVALMSDQK
EGDLKMEELK ILTSKESKTE NTQYSLQLWP DRKVQQITNF PHPYPQLASL QKEMIRYQRK
DGVQLTATLY LPPGYDPSKD GPLPCLFWSY PGEFKSKDAA GQVRGSPNEF AGIGSTSALL
WLARRFAILS GPTIPIIGEG DEEANDRYVE QLVASAEAAV EEVVRRGVAD RSKIAVGGHS
YGAFMTANLL AHAPHLFACG IARSGAYNRT LTPFGFQNED RTLWEATNVY VEMSPFMSAN
KIKKPILLIH GEEDNNPGTL TMQSDRFFNA LKGHGALCRL VVLPHESHGY SARESIMHVL
WETDRWLQKY CVPNTSDADT SPDQSKEGSD SADKVSTGTG GGNPEFGEHE VHSKLRRSLL
