ID   CGEP_ORYSJ              Reviewed;         938 AA.
AC   Q10MJ1; A0A0P0VWI9; B9F7Y1;
DT   05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT   22-AUG-2006, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Probable glutamyl endopeptidase, chloroplastic;
DE            EC=3.4.21.-;
DE   Flags: Precursor;
GN   Name=GEP; OrderedLocusNames=Os03g0307100, LOC_Os03g19410;
GN   ORFNames=OsJ_10563;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16109971; DOI=10.1101/gr.3869505;
RG   The rice chromosome 3 sequencing consortium;
RA   Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA   Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA   Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA   Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA   Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA   Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA   Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA   Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA   Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA   Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA   O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA   Jin W., Lee H.R., Jiang J., Jackson S.;
RT   "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT   and diverged grass species.";
RL   Genome Res. 15:1284-1291(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- FUNCTION: Serine-type protease active in vitro against the LHCII N-
CC       terminal. Cleaves its substrate on the carboxy-side of Glu residues (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase S9D family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AK102205; Type=Frameshift; Evidence={ECO:0000305};
CC       Sequence=EEE58916.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; DP000009; ABF95536.1; -; Genomic_DNA.
DR   EMBL; AP008209; BAF11807.1; -; Genomic_DNA.
DR   EMBL; AP014959; BAS83820.1; -; Genomic_DNA.
DR   EMBL; CM000140; EEE58916.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AK102205; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; XP_015630627.1; XM_015775141.1.
DR   AlphaFoldDB; Q10MJ1; -.
DR   BioGRID; 801643; 1.
DR   STRING; 4530.OS03T0307100-01; -.
DR   ESTHER; orysj-cgep; Glutamyl_Peptidase_S9.
DR   MEROPS; S09.021; -.
DR   PaxDb; Q10MJ1; -.
DR   PRIDE; Q10MJ1; -.
DR   EnsemblPlants; Os03t0307100-01; Os03t0307100-01; Os03g0307100.
DR   GeneID; 4332606; -.
DR   Gramene; Os03t0307100-01; Os03t0307100-01; Os03g0307100.
DR   KEGG; osa:4332606; -.
DR   eggNOG; KOG2100; Eukaryota.
DR   HOGENOM; CLU_017120_0_0_1; -.
DR   InParanoid; Q10MJ1; -.
DR   OMA; FPIQSER; -.
DR   OrthoDB; 265965at2759; -.
DR   Proteomes; UP000000763; Chromosome 3.
DR   Proteomes; UP000007752; Chromosome 3.
DR   Proteomes; UP000059680; Chromosome 3.
DR   Genevisible; Q10MJ1; OS.
DR   GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IBA:GO_Central.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR001375; Peptidase_S9.
DR   Pfam; PF00326; Peptidase_S9; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; Hydrolase; Plastid; Protease; Reference proteome;
KW   Serine protease; Transit peptide.
FT   TRANSIT         1..54
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           55..938
FT                   /note="Probable glutamyl endopeptidase, chloroplastic"
FT                   /id="PRO_0000397885"
FT   REGION          58..77
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          897..938
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        897..915
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        762
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        836
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        870
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   938 AA;  103895 MW;  14BFAECF52AAA021 CRC64;
     MSSLTILLQR ACLRFALLPV PPLRAPLRPP RRPLGLPRRS AMSSSAASRL SHIVAAAGGA
     AGESSEPPAA AAAASGLAQE DDDLSSAMMG YRLPPKEIQD IVDAPPLPVL SFSPSKDKIL
     FLKRRALPPL SDLAKPEEKL AGVRIDGYSN TRSRMSFYTG IGIHKLMDDG TLGPEKVVHG
     YPEGARINFV TWSQDGRHLS FSVRVDEEDN TSGKLRLWIA DVESGEARPL FKSPEIYLNA
     IFDSFVWVNN STLLVCTIPL SRGAPPQKPS VPSGPKIQSN ETSNVVQVRT FQDLLKDEYD
     ADLFDYYATS QLVLASFDGT VKPIGPPAVY TSIDPSPDDK YLMISSIHRP YSYIVPCGRF
     PKKVELWTVD GEFIRELCDL PLAEDIPIAT SSVRKGKRSI YWRPDKPAML YWVETQDGGD
     AKVEVSPRDI VYMENAEPIN GEQPEILHKL DLRYAGTSWC DESLALVYES WYKTRKTRTW
     VISPDKKDVS PRILFDRSSE DVYSDPGSPM LRRTAMGTYV IAKVKKQDEN TYILLNGMGA
     TPEGNVPFLD LFDINTGSKE RIWQSDKEKY YETVVALMSD KTDGELPLEK LKILTSKESK
     TENTQYYLQI WPEKKQVQIT DFPHPYPQLA SLYKEMIRYQ RKDGVQLTAT LYLPPGYDPS
     QDGPLPCLVW SYPGEFKSKD AAGQVRGSPN EFPGIGATSP LLWLARGFAI LSGPTIPIIG
     EGDEEANDRY VEQLVTSAEA AAEEVVRRGV AHPDKIAVGG HSYGAFMTAN LLAHAPHLFC
     CGIARSGAYN RTLTPFGFQN EDRTLWEATN TYVEMSPFMS ANKIKKPILL IHGEQDNNSG
     TLTMQSDRFF NALKGHGALS RLVILPFESH GYSARESIMH VLWETDRWLQ KYCLSGSSKT
     DSDSVADTEN KTVSASGGGA PCEGPEAEGF SSMQRSLL