ID   CGS2_ARATH              Reviewed;         412 AA.
AC   Q9C876; Q9C816;
DT   07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 124.
DE   RecName: Full=Probable cystathionine gamma-synthase 2 {ECO:0000305};
DE            EC=2.5.1.48 {ECO:0000250|UniProtKB:P55217};
GN   Name=CGS2 {ECO:0000305};
GN   OrderedLocusNames=At1g33320 {ECO:0000312|Araport:AT1G33320};
GN   ORFNames=F10C21.1 {ECO:0000312|EMBL:AAG51206.1},
GN   T16O9.8 {ECO:0000312|EMBL:AAG51279.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
CC   -!- FUNCTION: Catalyzes the first committed step of methionine (Met)
CC       biosynthesis. Catalyzes the formation of L-cystathionine from
CC       homoserine esters and L-cysteine, via a gamma-replacement reaction.
CC       {ECO:0000250|UniProtKB:P55217}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-cysteine + O-succinyl-L-homoserine = H(+) + L,L-
CC         cystathionine + succinate; Xref=Rhea:RHEA:20397, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30031, ChEBI:CHEBI:35235, ChEBI:CHEBI:57661,
CC         ChEBI:CHEBI:58161; EC=2.5.1.48;
CC         Evidence={ECO:0000250|UniProtKB:P55217};
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000250|UniProtKB:P55217};
CC       Note=Binds 1 pyridoxal 5'-phosphate per subunit.
CC       {ECO:0000250|UniProtKB:P55217};
CC   -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de novo
CC       pathway; L-cystathionine from O-succinyl-L-homoserine: step 1/1.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the trans-sulfuration enzymes family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG51206.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AC027035; AAG51279.1; -; Genomic_DNA.
DR   EMBL; AC051630; AAG51206.1; ALT_INIT; Genomic_DNA.
DR   EMBL; CP002684; AEE31583.1; -; Genomic_DNA.
DR   PIR; H86456; H86456.
DR   RefSeq; NP_001321971.1; NM_001333034.1.
DR   RefSeq; NP_174600.1; NM_103059.2.
DR   AlphaFoldDB; Q9C876; -.
DR   SMR; Q9C876; -.
DR   STRING; 3702.AT1G33320.1; -.
DR   PaxDb; Q9C876; -.
DR   PRIDE; Q9C876; -.
DR   ProMEX; Q9C876; -.
DR   EnsemblPlants; AT1G33320.1; AT1G33320.1; AT1G33320.
DR   GeneID; 840226; -.
DR   Gramene; AT1G33320.1; AT1G33320.1; AT1G33320.
DR   KEGG; ath:AT1G33320; -.
DR   Araport; AT1G33320; -.
DR   TAIR; locus:2006887; AT1G33320.
DR   eggNOG; KOG0053; Eukaryota.
DR   HOGENOM; CLU_018986_1_0_1; -.
DR   InParanoid; Q9C876; -.
DR   OrthoDB; 572061at2759; -.
DR   PhylomeDB; Q9C876; -.
DR   BioCyc; ARA:AT1G33320-MON; -.
DR   UniPathway; UPA00051; UER00077.
DR   PRO; PR:Q9C876; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q9C876; baseline and differential.
DR   Genevisible; Q9C876; AT.
DR   GO; GO:0003962; F:cystathionine gamma-synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0102028; F:cystathionine gamma-synthase activity (acts on O-phosphohomoserine); IEA:UniProtKB-EC.
DR   GO; GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
DR   GO; GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0019346; P:transsulfuration; IEA:InterPro.
DR   CDD; cd00614; CGS_like; 1.
DR   Gene3D; 3.40.640.10; -; 1.
DR   Gene3D; 3.90.1150.10; -; 1.
DR   InterPro; IPR044639; CGS1/2.
DR   InterPro; IPR000277; Cys/Met-Metab_PyrdxlP-dep_enz.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR   PANTHER; PTHR43379; PTHR43379; 1.
DR   Pfam; PF01053; Cys_Met_Meta_PP; 1.
DR   PIRSF; PIRSF001434; CGS; 1.
DR   SUPFAM; SSF53383; SSF53383; 1.
DR   PROSITE; PS00868; CYS_MET_METAB_PP; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Methionine biosynthesis; Pyridoxal phosphate;
KW   Reference proteome; Transferase.
FT   CHAIN           1..412
FT                   /note="Probable cystathionine gamma-synthase 2"
FT                   /id="PRO_0000431692"
FT   MOD_RES         229
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000250|UniProtKB:P53780"
SQ   SEQUENCE   412 AA;  46004 MW;  6C9B1A47530CB41C CRC64;
     MQILKENASN QRFVTRESEV NRICCGFNGF QLGERLRRDM KTSSITTLVV NTTTYFFKDT
     TELIDFKEKR IDLYEYARYG NPTTMALEEK ISVLEGAEST LVMASGMYAS NVMLLALVPT
     NGHIVATKDC YKETRIFMEN FLTKLGITVT FIDSDDIAGL QTLVNNHEVS LFFTESPTNP
     FLRCVDIKLV SKICHRRGTL VCIDATIATP INQKTLALGA DLVHHSATKY IGGHNDFLAG
     SISGSMELVS KIRNLHKLLG GTLNPNAAYL LIRGMKTMHL RVRQQNSTGM KMAQVLEAHP
     KVSRVYYLGL PSHPEHLIAK RQMTGIGGLI SFEIDGDLKT TIKFIDALKI PYLAASFGGC
     ESLVDQLATG IWDIPREERL KDGFQDNLVR FSFGIEDFED IKADVLQALE TI