ACDH2_AZOSB
ID ACDH2_AZOSB Reviewed; 306 AA.
AC A1K6Y5;
DT 03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=Acetaldehyde dehydrogenase 2/3 {ECO:0000255|HAMAP-Rule:MF_01657};
DE EC=1.2.1.10 {ECO:0000255|HAMAP-Rule:MF_01657};
DE AltName: Full=Acetaldehyde dehydrogenase [acetylating] 2/3 {ECO:0000255|HAMAP-Rule:MF_01657};
GN Name=mhpF; OrderedLocusNames=azo1973;
GN and
GN Name=nahO; OrderedLocusNames=azo2432;
OS Azoarcus sp. (strain BH72).
OC Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Zoogloeaceae;
OC Azoarcus.
OX NCBI_TaxID=418699;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BH72;
RX PubMed=17057704; DOI=10.1038/nbt1243;
RA Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J.,
RA Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C.,
RA Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J.,
RA Weidner S., Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A.;
RT "Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp.
RT strain BH72.";
RL Nat. Biotechnol. 24:1385-1391(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetaldehyde + CoA + NAD(+) = acetyl-CoA + H(+) + NADH;
CC Xref=Rhea:RHEA:23288, ChEBI:CHEBI:15343, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945; EC=1.2.1.10; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01657};
CC -!- SIMILARITY: Belongs to the acetaldehyde dehydrogenase family.
CC {ECO:0000255|HAMAP-Rule:MF_01657}.
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DR EMBL; AM406670; CAL94590.1; -; Genomic_DNA.
DR EMBL; AM406670; CAL95049.1; -; Genomic_DNA.
DR RefSeq; WP_011765706.1; NC_008702.1.
DR AlphaFoldDB; A1K6Y5; -.
DR SMR; A1K6Y5; -.
DR STRING; 62928.azo1973; -.
DR EnsemblBacteria; CAL94590; CAL94590; azo1973.
DR EnsemblBacteria; CAL95049; CAL95049; azo2432.
DR KEGG; azo:azo1973; -.
DR KEGG; azo:azo2432; -.
DR eggNOG; COG4569; Bacteria.
DR HOGENOM; CLU_062208_0_0_4; -.
DR OMA; TSAYVHK; -.
DR OrthoDB; 1432332at2; -.
DR Proteomes; UP000002588; Chromosome.
DR GO; GO:0008774; F:acetaldehyde dehydrogenase (acetylating) activity; IEA:UniProtKB-UniRule.
DR GO; GO:0051287; F:NAD binding; IEA:UniProtKB-UniRule.
DR GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01657; Ac_ald_DH_ac; 1.
DR InterPro; IPR003361; Acetaldehyde_dehydrogenase.
DR InterPro; IPR015426; Acetylaldehyde_DH_C.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR000534; Semialdehyde_DH_NAD-bd.
DR Pfam; PF09290; AcetDehyd-dimer; 1.
DR Pfam; PF01118; Semialdhyde_dh; 1.
DR PIRSF; PIRSF015689; Actaldh_dh_actl; 1.
DR SMART; SM00859; Semialdhyde_dh; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
DR TIGRFAMs; TIGR03215; ac_ald_DH_ac; 1.
PE 3: Inferred from homology;
KW Aromatic hydrocarbons catabolism; NAD; Oxidoreductase; Reference proteome.
FT CHAIN 1..306
FT /note="Acetaldehyde dehydrogenase 2/3"
FT /id="PRO_0000387618"
FT ACT_SITE 130
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01657"
FT BINDING 161..169
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01657"
FT BINDING 272
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01657"
SQ SEQUENCE 306 AA; 32572 MW; CC8ED08B6AB51E4F CRC64;
MNKIKCALIG PGNIGTDLLY KLKRSPVLEP VWMVGIDAAS EGLARARELG LKTTADGVDG
LLPHVKADGV QIAFDATSAY VHAENSRKLN ELGVLMIDLT PAAIGPFCVP PVNLKDHVGR
GEMNVNMVTC GGQATIPMVA AVSRVQPVKY GEIVATVSSK SAGPGTRKNI DEFTRTTAGA
VEKVGGAKVG KAIIILNPAE PPLIMRDTVH CLTETEPDQQ KITESIHAMI REVQKYVPGY
KLVNGPVFDG NRVSIYLEVE GLGDYLPKYA GNLDIMTAAG ARTAEMFAEE ILAGRLTLES
NRAVLA
