ID   CH60B_ARATH             Reviewed;         585 AA.
AC   Q8L7B5; O49314;
DT   24-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 135.
DE   RecName: Full=Chaperonin CPN60-like 1, mitochondrial;
DE   AltName: Full=HSP60-like 1;
DE   Flags: Precursor;
GN   OrderedLocusNames=At2g33210; ORFNames=F25I18.5;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP   ANALYSIS].
RC   STRAIN=cv. Landsberg erecta;
RX   PubMed=14671022; DOI=10.1105/tpc.016055;
RA   Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J.,
RA   Millar A.H.;
RT   "Experimental analysis of the Arabidopsis mitochondrial proteome highlights
RT   signaling and regulatory components, provides assessment of targeting
RT   prediction programs, and indicates plant-specific mitochondrial proteins.";
RL   Plant Cell 16:241-256(2004).
RN   [6]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=cv. Landsberg erecta;
RX   PubMed=17272265; DOI=10.1074/mcp.m600408-mcp200;
RA   Maor R., Jones A., Nuehse T.S., Studholme D.J., Peck S.C., Shirasu K.;
RT   "Multidimensional protein identification technology (MudPIT) analysis of
RT   ubiquitinated proteins in plants.";
RL   Mol. Cell. Proteomics 6:601-610(2007).
CC   -!- FUNCTION: Implicated in mitochondrial protein import and macromolecular
CC       assembly. May facilitate the correct folding of imported proteins. May
CC       also prevent misfolding and promote the refolding and proper assembly
CC       of unfolded polypeptides generated under stress conditions in the
CC       mitochondrial matrix (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:14671022}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=1;
CC         Comment=A number of isoforms are produced. According to EST
CC         sequences.;
CC       Name=1;
CC         IsoId=Q8L7B5-1; Sequence=Displayed;
CC   -!- SIMILARITY: Belongs to the chaperonin (HSP60) family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAC04902.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC002334; AAC04902.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002685; AEC08799.1; -; Genomic_DNA.
DR   EMBL; AY136360; AAM97026.1; -; mRNA.
DR   EMBL; BT000103; AAN15422.1; -; mRNA.
DR   EMBL; AK175415; BAD43178.1; -; mRNA.
DR   PIR; F84742; F84742.
DR   RefSeq; NP_850203.1; NM_179872.3. [Q8L7B5-1]
DR   AlphaFoldDB; Q8L7B5; -.
DR   SMR; Q8L7B5; -.
DR   BioGRID; 3230; 67.
DR   IntAct; Q8L7B5; 2.
DR   STRING; 3702.AT2G33210.1; -.
DR   MetOSite; Q8L7B5; -.
DR   PaxDb; Q8L7B5; -.
DR   PRIDE; Q8L7B5; -.
DR   EnsemblPlants; AT2G33210.1; AT2G33210.1; AT2G33210. [Q8L7B5-1]
DR   GeneID; 817883; -.
DR   Gramene; AT2G33210.1; AT2G33210.1; AT2G33210. [Q8L7B5-1]
DR   KEGG; ath:AT2G33210; -.
DR   Araport; AT2G33210; -.
DR   TAIR; locus:2046590; AT2G33210.
DR   eggNOG; KOG0356; Eukaryota.
DR   HOGENOM; CLU_016503_3_0_1; -.
DR   InParanoid; Q8L7B5; -.
DR   OrthoDB; 415781at2759; -.
DR   PhylomeDB; Q8L7B5; -.
DR   PRO; PR:Q8L7B5; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; Q8L7B5; baseline and differential.
DR   Genevisible; Q8L7B5; AT.
DR   GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR   GO; GO:0009570; C:chloroplast stroma; HDA:TAIR.
DR   GO; GO:0005829; C:cytosol; HDA:TAIR.
DR   GO; GO:0005783; C:endoplasmic reticulum; HDA:TAIR.
DR   GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR   GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR   GO; GO:0005524; F:ATP binding; HDA:TAIR.
DR   GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR   GO; GO:0005507; F:copper ion binding; HDA:TAIR.
DR   GO; GO:0003723; F:RNA binding; IPI:TAIR.
DR   GO; GO:0006457; P:protein folding; IBA:GO_Central.
DR   GO; GO:0042026; P:protein refolding; IEA:InterPro.
DR   CDD; cd03344; GroEL; 1.
DR   Gene3D; 1.10.560.10; -; 1.
DR   Gene3D; 3.30.260.10; -; 1.
DR   Gene3D; 3.50.7.10; -; 1.
DR   HAMAP; MF_00600; CH60; 1.
DR   InterPro; IPR018370; Chaperonin_Cpn60_CS.
DR   InterPro; IPR001844; Cpn60/GroEL.
DR   InterPro; IPR002423; Cpn60/GroEL/TCP-1.
DR   InterPro; IPR027409; GroEL-like_apical_dom_sf.
DR   InterPro; IPR027413; GROEL-like_equatorial_sf.
DR   InterPro; IPR027410; TCP-1-like_intermed_sf.
DR   Pfam; PF00118; Cpn60_TCP1; 1.
DR   PRINTS; PR00298; CHAPERONIN60.
DR   SUPFAM; SSF48592; SSF48592; 1.
DR   SUPFAM; SSF52029; SSF52029; 1.
DR   SUPFAM; SSF54849; SSF54849; 1.
DR   TIGRFAMs; TIGR02348; GroEL; 1.
DR   PROSITE; PS00296; CHAPERONINS_CPN60; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; ATP-binding; Chaperone; Mitochondrion;
KW   Nucleotide-binding; Reference proteome; Stress response; Transit peptide.
FT   TRANSIT         1..32
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           33..585
FT                   /note="Chaperonin CPN60-like 1, mitochondrial"
FT                   /id="PRO_0000045792"
SQ   SEQUENCE   585 AA;  61978 MW;  A512B6975E674B4A CRC64;
     MYRLVSNVAS KARIARKCTS QIGSRLNSTR NYAAKDIRFG VEARALMLRG VEDLADAVKV
     TMGPKGRNVI IEQSWGAPKV TKDGVTVAKS IEFKDRIKNV GASLVKQVAN ATNDVAGDGT
     TCATVLTRAI FTEGCKSVAA GMNAMDLRRG IKLAVDTVVT NLQSRARMIS TSEEIAQVGT
     ISANGDREIG ELIAKAMETV GKEGVITIQD GKTLFNELEV VEGMKIDRGY ISPYFITNPK
     TQKCELEDPL ILIHEKKISN INAMVKVLEL ALKKQRPLLI VAEDVESDAL ATLILNKLRA
     NIKVCAVKAP GFGENRKANL HDLAALTGAQ VITEELGMNL DNIDLSMFGN CKKVTVSKDD
     TVVLDGAGDK QAIGERCEQI RSMVEASTSD YDKEKLQERL AKLSGGVAVL KIGGASETEV
     SEKKDRVTDA LNATKAAVEE GIVPGGGVAL LYASKELEKL STANFDQKIG VQIIQNALKT
     PVYTIASNAG VEGAVVVGKL LEQDNPDLGY DAAKGEYVDM IKAGIIDPLK VIRTALVDAA
     SVSSLLTTTE AVVTEIPTKE VASPGMGGGG MGGMGGMGGM GGMGF