CH60B_DROME
ID CH60B_DROME Reviewed; 648 AA.
AC Q9VPS5; Q53YH7; Q961V0;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=60 kDa heat shock protein homolog 1, mitochondrial;
DE AltName: Full=60 kDa chaperonin;
DE AltName: Full=CPN60;
DE AltName: Full=Heat shock protein 60;
DE Short=HSP-60;
DE AltName: Full=Hsp60;
DE Flags: Precursor;
GN Name=Hsp60B; ORFNames=CG2830;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Prevents misfolding and promotes the refolding and proper
CC assembly of unfolded polypeptides generated under stress conditions.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the chaperonin (HSP60) family. {ECO:0000305}.
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DR EMBL; AE014134; AAF51467.1; -; Genomic_DNA.
DR EMBL; AY047544; AAK77276.1; -; mRNA.
DR EMBL; BT001426; AAN71181.1; -; mRNA.
DR RefSeq; NP_524925.1; NM_080186.4.
DR AlphaFoldDB; Q9VPS5; -.
DR SMR; Q9VPS5; -.
DR BioGRID; 71509; 39.
DR DIP; DIP-17907N; -.
DR IntAct; Q9VPS5; 4.
DR STRING; 7227.FBpp0077665; -.
DR PaxDb; Q9VPS5; -.
DR PRIDE; Q9VPS5; -.
DR DNASU; 48572; -.
DR EnsemblMetazoa; FBtr0078000; FBpp0077665; FBgn0011244.
DR GeneID; 48572; -.
DR KEGG; dme:Dmel_CG2830; -.
DR CTD; 48572; -.
DR FlyBase; FBgn0011244; Hsp60B.
DR VEuPathDB; VectorBase:FBgn0011244; -.
DR eggNOG; KOG0356; Eukaryota.
DR GeneTree; ENSGT00390000005727; -.
DR HOGENOM; CLU_016503_3_0_1; -.
DR InParanoid; Q9VPS5; -.
DR OMA; LKDQHMN; -.
DR OrthoDB; 415781at2759; -.
DR PhylomeDB; Q9VPS5; -.
DR SignaLink; Q9VPS5; -.
DR BioGRID-ORCS; 48572; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Hsp60B; fly.
DR GenomeRNAi; 48572; -.
DR PRO; PR:Q9VPS5; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0011244; Expressed in testis and 15 other tissues.
DR Genevisible; Q9VPS5; DM.
DR GO; GO:0005743; C:mitochondrial inner membrane; IBA:GO_Central.
DR GO; GO:0005759; C:mitochondrial matrix; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; ISS:FlyBase.
DR GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR GO; GO:0051087; F:chaperone binding; IBA:GO_Central.
DR GO; GO:0008637; P:apoptotic mitochondrial changes; IBA:GO_Central.
DR GO; GO:0034514; P:mitochondrial unfolded protein response; IBA:GO_Central.
DR GO; GO:0006457; P:protein folding; IBA:GO_Central.
DR GO; GO:0045041; P:protein import into mitochondrial intermembrane space; IBA:GO_Central.
DR GO; GO:0042026; P:protein refolding; IEA:InterPro.
DR CDD; cd03344; GroEL; 1.
DR Gene3D; 1.10.560.10; -; 1.
DR Gene3D; 3.30.260.10; -; 1.
DR Gene3D; 3.50.7.10; -; 1.
DR InterPro; IPR018370; Chaperonin_Cpn60_CS.
DR InterPro; IPR001844; Cpn60/GroEL.
DR InterPro; IPR002423; Cpn60/GroEL/TCP-1.
DR InterPro; IPR027409; GroEL-like_apical_dom_sf.
DR InterPro; IPR027413; GROEL-like_equatorial_sf.
DR InterPro; IPR027410; TCP-1-like_intermed_sf.
DR Pfam; PF00118; Cpn60_TCP1; 1.
DR PRINTS; PR00298; CHAPERONIN60.
DR SUPFAM; SSF48592; SSF48592; 1.
DR SUPFAM; SSF52029; SSF52029; 1.
DR SUPFAM; SSF54849; SSF54849; 2.
DR TIGRFAMs; TIGR02348; GroEL; 1.
DR PROSITE; PS00296; CHAPERONINS_CPN60; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Chaperone; Mitochondrion; Nucleotide-binding;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..55
FT /note="Mitochondrion"
FT /evidence="ECO:0000250"
FT CHAIN 56..648
FT /note="60 kDa heat shock protein homolog 1, mitochondrial"
FT /id="PRO_0000005032"
FT CONFLICT 199
FT /note="K -> N (in Ref. 3; AAK77276)"
FT /evidence="ECO:0000305"
FT CONFLICT 581..589
FT /note="Missing (in Ref. 3; AAK77276)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 648 AA; 68637 MW; E45D44A166C78455 CRC64;
MFRSCVPKAI TSSRCFARMY SKDVRFGSGV RAMMIRGVDI LADAVAVTMG PKGRSVIVER
PWTSPKITKD GFTVARSIAL KDQHMNLGAK LVQDVADNTN ESAGDGTTTA TVLARAIAKE
GFNQITMGAN PVEIRRGVML AVDVVKDKLK EMSKAVETRE EIQQVATLSA NGDTEIGRLI
GEATDKVGPR GTITVKDGKR LKDELNIIQG LRFDNGYVSP FFVNSSKGSK VEFANALVMI
SLKKITGLSQ IVKGLEQSLR QRRPLIIIAE DISGEALNAL VLNKLRLGLQ VCAVKSPSYG
HHRKELIGDI SAATGATIFG DDINYSKMEE AKLEDLGQVG EAVISKDSTM LLQGKPKTGL
LEMRIQQIQD ELAEKQIKPE QRDRLRQRLS ALTKGVAVLH IGGGSEVEVN EKKDRVVDAL
NATRAAIEEG IVPGGGTAFL RCIPYLQELK TESADLQKGV DIVCNALRMP CQTIAQNAGV
DGPMVVAKVL NGSEDYGYDA MGDEYCRLVE KGIIDPTKVL RTAITDAAGV ASLLSTTEVV
ITDSRNDDLL SKLSGAGGGM DDGLDMNMGG LEELAALSGL GGMGGMGGMG GMGGMGGMGG
GFGGMGAGGG MSASASNDGP TAEEMNEMVK AIPGMEQVEV RDIDSGMM
