CH60C_DROME
ID CH60C_DROME Reviewed; 576 AA.
AC Q9VMN5; A4V087; Q8IHD0; Q8MZB0;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2004, sequence version 2.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=60 kDa heat shock protein homolog 2, mitochondrial;
DE AltName: Full=60 kDa chaperonin;
DE AltName: Full=CPN60;
DE AltName: Full=Heat shock protein 60;
DE Short=HSP-60;
DE AltName: Full=Hsp60;
DE Flags: Precursor;
GN Name=Hsp60C; ORFNames=CG7235;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Testis;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=16385159; DOI=10.1007/bf02715797;
RA Sarkar S., Lakhotia S.C.;
RT "The Hsp60C gene in the 25F cytogenetic region in Drosophila melanogaster
RT is essential for tracheal development and fertility.";
RL J. Genet. 84:265-281(2005).
CC -!- FUNCTION: Prevents misfolding and promotes the refolding and proper
CC assembly of unfolded polypeptides generated under stress conditions (By
CC similarity). Essential for proper development of trachea, spermatogonia
CC and spermatocytes. {ECO:0000250, ECO:0000269|PubMed:16385159}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: First detectable expression is seen in the
CC posterior part of the dorsal tracheal trunk at stage 14-15, which marks
CC the beginning of terminal tracheation. In the larval gut, expression in
CC proventriculus is stronger than in midgut and hindgut. Malpighian
CC tubules shows low expression and late third instar larval imaginal
CC disks and brain showed moderate expression. In larval ovary and testis,
CC expression is strong in the posterior region.
CC {ECO:0000269|PubMed:16385159}.
CC -!- DEVELOPMENTAL STAGE: Expression first seen in late embryonic stages and
CC continues in larval and adult stages. {ECO:0000269|PubMed:16385159}.
CC -!- DISRUPTION PHENOTYPE: Flies are male and female sterile; reduction in
CC numbers of primary and secondary spermatocytes.
CC {ECO:0000269|PubMed:16385159}.
CC -!- SIMILARITY: Belongs to the chaperonin (HSP60) family. {ECO:0000305}.
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DR EMBL; AE014134; AAF52277.2; -; Genomic_DNA.
DR EMBL; AE014134; AAN10550.2; -; Genomic_DNA.
DR EMBL; AE014134; AAN10551.2; -; Genomic_DNA.
DR EMBL; AY113273; AAM29278.1; -; mRNA.
DR EMBL; BT001308; AAN71063.1; -; mRNA.
DR RefSeq; NP_608948.2; NM_135104.5.
DR RefSeq; NP_723104.2; NM_164654.2.
DR RefSeq; NP_723105.2; NM_164655.3.
DR AlphaFoldDB; Q9VMN5; -.
DR SMR; Q9VMN5; -.
DR BioGRID; 59964; 15.
DR DIP; DIP-20697N; -.
DR IntAct; Q9VMN5; 14.
DR STRING; 7227.FBpp0078742; -.
DR PaxDb; Q9VMN5; -.
DR PRIDE; Q9VMN5; -.
DR DNASU; 33796; -.
DR EnsemblMetazoa; FBtr0079110; FBpp0078742; FBgn0031728.
DR EnsemblMetazoa; FBtr0079111; FBpp0078743; FBgn0031728.
DR EnsemblMetazoa; FBtr0079112; FBpp0078744; FBgn0031728.
DR GeneID; 33796; -.
DR KEGG; dme:Dmel_CG7235; -.
DR UCSC; CG7235-RA; d. melanogaster.
DR CTD; 33796; -.
DR FlyBase; FBgn0031728; Hsp60C.
DR VEuPathDB; VectorBase:FBgn0031728; -.
DR eggNOG; KOG0356; Eukaryota.
DR GeneTree; ENSGT00390000005727; -.
DR HOGENOM; CLU_016503_3_0_1; -.
DR InParanoid; Q9VMN5; -.
DR OMA; PYILINQ; -.
DR OrthoDB; 415781at2759; -.
DR PhylomeDB; Q9VMN5; -.
DR SignaLink; Q9VMN5; -.
DR BioGRID-ORCS; 33796; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Hsp60C; fly.
DR GenomeRNAi; 33796; -.
DR PRO; PR:Q9VMN5; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0031728; Expressed in testis and 17 other tissues.
DR Genevisible; Q9VMN5; DM.
DR GO; GO:0005743; C:mitochondrial inner membrane; IBA:GO_Central.
DR GO; GO:0005759; C:mitochondrial matrix; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; ISS:FlyBase.
DR GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR GO; GO:0051087; F:chaperone binding; IBA:GO_Central.
DR GO; GO:0008637; P:apoptotic mitochondrial changes; IBA:GO_Central.
DR GO; GO:0007301; P:female germline ring canal formation; IMP:FlyBase.
DR GO; GO:0034514; P:mitochondrial unfolded protein response; IBA:GO_Central.
DR GO; GO:0048477; P:oogenesis; IMP:FlyBase.
DR GO; GO:0007424; P:open tracheal system development; IMP:FlyBase.
DR GO; GO:0030707; P:ovarian follicle cell development; IMP:FlyBase.
DR GO; GO:0006457; P:protein folding; IBA:GO_Central.
DR GO; GO:0045041; P:protein import into mitochondrial intermembrane space; IBA:GO_Central.
DR GO; GO:0042026; P:protein refolding; IEA:InterPro.
DR GO; GO:0007283; P:spermatogenesis; IMP:FlyBase.
DR CDD; cd03344; GroEL; 1.
DR Gene3D; 1.10.560.10; -; 1.
DR Gene3D; 3.30.260.10; -; 1.
DR Gene3D; 3.50.7.10; -; 1.
DR HAMAP; MF_00600; CH60; 1.
DR InterPro; IPR018370; Chaperonin_Cpn60_CS.
DR InterPro; IPR001844; Cpn60/GroEL.
DR InterPro; IPR002423; Cpn60/GroEL/TCP-1.
DR InterPro; IPR027409; GroEL-like_apical_dom_sf.
DR InterPro; IPR027413; GROEL-like_equatorial_sf.
DR InterPro; IPR027410; TCP-1-like_intermed_sf.
DR Pfam; PF00118; Cpn60_TCP1; 1.
DR PRINTS; PR00298; CHAPERONIN60.
DR SUPFAM; SSF48592; SSF48592; 1.
DR SUPFAM; SSF52029; SSF52029; 1.
DR SUPFAM; SSF54849; SSF54849; 1.
DR TIGRFAMs; TIGR02348; GroEL; 1.
DR PROSITE; PS00296; CHAPERONINS_CPN60; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Chaperone; Developmental protein; Differentiation;
KW Mitochondrion; Nucleotide-binding; Oogenesis; Reference proteome;
KW Spermatogenesis; Transit peptide.
FT TRANSIT 1..61
FT /note="Mitochondrion"
FT /evidence="ECO:0000250"
FT CHAIN 62..576
FT /note="60 kDa heat shock protein homolog 2, mitochondrial"
FT /id="PRO_0000005033"
FT CONFLICT 160
FT /note="R -> Q (in Ref. 3; AAM29278)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 576 AA; 61587 MW; 7FB52C049E9A5F79 CRC64;
MMRMFRYTNT LQRTAKISHV LWARNYAKDV RFGPEVRAMM LQGVDVLADA VAVTMGPKGR
NVIIEQSWGS PKITKDGVTV AKSIALKDKF QNIGAKLVQD VANNTNEEAG DGTTTATVLA
RAIAKEGFEK ISRGASPVEI RRGVMLAIET VKDNLRRLSR PVNTPEEICQ VATISANGDK
SVGNLISEAI KKVGRDGVIT VKDGKTLCDE LEVIEGMKFD RGYISPYFIN TSKGAKVEFQ
DALLLFCEKK IKSAPSIVPA LELANAQRKP LVIIAEDLEA EALSTLVVNR LKVGLQVCAV
KAPGFGDNRK ENLMDMAVAT GGIVFGDEAN MVRLEDIKMS DFGRVGEVVV SKDDTMLLKG
KGQKAEVEKR VEGLREAIKE STSSYEKEKM QERLARLSSG VALLRVGGSS DVEVSEKKDR
VIDALNATRA AVEEGIVPGG GTALLRCIQK LNDLKGANED QNMGIEIIRR ALRMPCLTIA
KNAGVDGAMV VAKVEILDGD YGYDALKGEY GNMIERGIID PTKVVRTAIS DAAGVASLLT
TAEAVVTELP LEEAAAAGAA AGLGALGGMG MGGMGM
