CHAA_BACSU
ID CHAA_BACSU Reviewed; 351 AA.
AC O34840; Q79EY2;
DT 16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Ca(2+)/H(+) antiporter ChaA;
GN Name=chaA; Synonyms=yfkE; OrderedLocusNames=BSU07920;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / AC327;
RX PubMed=8969503; DOI=10.1099/13500872-142-11-3057;
RA Yamamoto H., Uchiyama S., Sekiguchi J.;
RT "Cloning and sequencing of a 40.6 kb segment in the 73 degrees-76 degrees
RT region of the Bacillus subtilis chromosome containing genes for trehalose
RT metabolism and acetoin utilization.";
RL Microbiology 142:3057-3065(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION, AND GENE NAME.
RC STRAIN=168;
RX PubMed=19543710; DOI=10.1007/s00203-009-0494-7;
RA Fujisawa M., Wada Y., Tsuchiya T., Ito M.;
RT "Characterization of Bacillus subtilis YfkE (ChaA): a calcium-specific
RT Ca2+/H+ antiporter of the CaCA family.";
RL Arch. Microbiol. 191:649-657(2009).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS), ACTIVITY REGULATION, SUBUNIT,
RP SUBCELLULAR LOCATION, AND MUTAGENESIS OF ASN-64; GLY-68; ASN-69; GLU-72;
RP ASN-99; LYS-116; ASN-252; GLU-255; HIS-256; SER-258; SER-278 AND GLN-281.
RX PubMed=23798403; DOI=10.1073/pnas.1302515110;
RA Wu M., Tong S., Waltersperger S., Diederichs K., Wang M., Zheng L.;
RT "Crystal structure of Ca2+/H+ antiporter protein YfkE reveals the
RT mechanisms of Ca2+ efflux and its pH regulation.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:11367-11372(2013).
CC -!- FUNCTION: Ca(+)/H(+) antiporter that extrudes calcium in exchange for
CC external protons. Does not transport sodium or potassium.
CC {ECO:0000269|PubMed:19543710}.
CC -!- ACTIVITY REGULATION: Calcium efflux is tightly regulated by
CC intracellular pH. {ECO:0000269|PubMed:23798403}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=12.5 uM for Ca(2+) (at pH 8.5) {ECO:0000269|PubMed:19543710};
CC KM=37.5 uM for Ca(2+) (at pH 8.0) {ECO:0000269|PubMed:19543710};
CC KM=113 uM for Ca(2+) (at pH 7.5) {ECO:0000269|PubMed:19543710};
CC -!- SUBUNIT: Homotrimer. {ECO:0000269|PubMed:23798403}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:23798403};
CC Multi-pass membrane protein {ECO:0000269|PubMed:23798403}.
CC -!- INDUCTION: Transcriptionally regulated by the forespore-specific sigma
CC factor, SigG, and the general stress response regulator, SigB.
CC {ECO:0000269|PubMed:19543710}.
CC -!- SIMILARITY: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19)
CC family. Cation/proton exchanger (CAX) subfamily. {ECO:0000305}.
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DR EMBL; D83967; BAA23395.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12621.1; -; Genomic_DNA.
DR PIR; A69808; A69808.
DR RefSeq; NP_388673.1; NC_000964.3.
DR RefSeq; WP_003242902.1; NZ_JNCM01000032.1.
DR PDB; 4KJR; X-ray; 3.00 A; A/B=1-351.
DR PDB; 4KJS; X-ray; 3.05 A; A/B=1-351.
DR PDBsum; 4KJR; -.
DR PDBsum; 4KJS; -.
DR AlphaFoldDB; O34840; -.
DR SMR; O34840; -.
DR STRING; 224308.BSU07920; -.
DR TCDB; 2.A.19.2.11; the ca(2+):cation antiporter (caca) family.
DR TCDB; 2.A.19.2.7; the ca(2+):cation antiporter (caca) family.
DR PaxDb; O34840; -.
DR EnsemblBacteria; CAB12621; CAB12621; BSU_07920.
DR GeneID; 939693; -.
DR KEGG; bsu:BSU07920; -.
DR PATRIC; fig|224308.179.peg.858; -.
DR eggNOG; COG0387; Bacteria.
DR InParanoid; O34840; -.
DR OMA; AAVMITC; -.
DR PhylomeDB; O34840; -.
DR BioCyc; BSUB:BSU07920-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:UniProtKB.
DR GO; GO:0015369; F:calcium:proton antiporter activity; IDA:UniProtKB.
DR GO; GO:0070588; P:calcium ion transmembrane transport; IDA:UniProtKB.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; IBA:GO_Central.
DR Gene3D; 1.20.1420.30; -; 1.
DR InterPro; IPR004713; CaH_exchang.
DR InterPro; IPR004798; CAX-like.
DR InterPro; IPR004837; NaCa_Exmemb.
DR InterPro; IPR044880; NCX_ion-bd_dom_sf.
DR PANTHER; PTHR31503; PTHR31503; 1.
DR Pfam; PF01699; Na_Ca_ex; 2.
DR TIGRFAMs; TIGR00378; cax; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antiport; Calcium; Calcium transport; Cell membrane;
KW Ion transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..351
FT /note="Ca(2+)/H(+) antiporter ChaA"
FT /id="PRO_0000376844"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 25..45
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 86..106
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 130..150
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 156..176
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 205..225
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 241..261
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..302
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 303..323
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 331..351
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MUTAGEN 64
FT /note="N->A: Almost no change in activity."
FT /evidence="ECO:0000269|PubMed:23798403"
FT MUTAGEN 68
FT /note="G->A: Decrease in activity."
FT /evidence="ECO:0000269|PubMed:23798403"
FT MUTAGEN 69
FT /note="N->A: Almost loss of activity."
FT /evidence="ECO:0000269|PubMed:23798403"
FT MUTAGEN 72
FT /note="E->A: Lack of activity."
FT /evidence="ECO:0000269|PubMed:23798403"
FT MUTAGEN 99
FT /note="N->A: Decrease in activity."
FT /evidence="ECO:0000269|PubMed:23798403"
FT MUTAGEN 116
FT /note="K->A: Almost no change in activity."
FT /evidence="ECO:0000269|PubMed:23798403"
FT MUTAGEN 252
FT /note="N->A: Decrease in activity."
FT /evidence="ECO:0000269|PubMed:23798403"
FT MUTAGEN 255
FT /note="E->A: Lack of activity."
FT /evidence="ECO:0000269|PubMed:23798403"
FT MUTAGEN 256
FT /note="H->A: Almost loss of activity."
FT /evidence="ECO:0000269|PubMed:23798403"
FT MUTAGEN 258
FT /note="S->A: Decrease in activity."
FT /evidence="ECO:0000269|PubMed:23798403"
FT MUTAGEN 278
FT /note="S->A: Almost no change in activity."
FT /evidence="ECO:0000269|PubMed:23798403"
FT MUTAGEN 281
FT /note="Q->A: Almost loss of activity."
FT /evidence="ECO:0000269|PubMed:23798403"
FT HELIX 3..22
FT /evidence="ECO:0007829|PDB:4KJR"
FT HELIX 26..53
FT /evidence="ECO:0007829|PDB:4KJR"
FT HELIX 60..66
FT /evidence="ECO:0007829|PDB:4KJR"
FT HELIX 67..69
FT /evidence="ECO:0007829|PDB:4KJR"
FT HELIX 70..81
FT /evidence="ECO:0007829|PDB:4KJR"
FT HELIX 85..99
FT /evidence="ECO:0007829|PDB:4KJR"
FT HELIX 102..110
FT /evidence="ECO:0007829|PDB:4KJR"
FT TURN 111..113
FT /evidence="ECO:0007829|PDB:4KJS"
FT HELIX 121..139
FT /evidence="ECO:0007829|PDB:4KJR"
FT HELIX 141..146
FT /evidence="ECO:0007829|PDB:4KJR"
FT TURN 147..149
FT /evidence="ECO:0007829|PDB:4KJR"
FT HELIX 152..176
FT /evidence="ECO:0007829|PDB:4KJR"
FT HELIX 204..212
FT /evidence="ECO:0007829|PDB:4KJR"
FT TURN 213..215
FT /evidence="ECO:0007829|PDB:4KJR"
FT HELIX 216..234
FT /evidence="ECO:0007829|PDB:4KJR"
FT HELIX 239..244
FT /evidence="ECO:0007829|PDB:4KJR"
FT HELIX 246..256
FT /evidence="ECO:0007829|PDB:4KJR"
FT HELIX 258..261
FT /evidence="ECO:0007829|PDB:4KJR"
FT STRAND 263..266
FT /evidence="ECO:0007829|PDB:4KJR"
FT HELIX 269..284
FT /evidence="ECO:0007829|PDB:4KJR"
FT HELIX 286..296
FT /evidence="ECO:0007829|PDB:4KJR"
FT STRAND 297..299
FT /evidence="ECO:0007829|PDB:4KJS"
FT HELIX 307..324
FT /evidence="ECO:0007829|PDB:4KJR"
FT HELIX 333..350
FT /evidence="ECO:0007829|PDB:4KJR"
SQ SEQUENCE 351 AA; 37522 MW; D81E4C5FE6081767 CRC64;
MNRIFFILVA AGVPLSVIGS LMHWPSAVLF AVYCVTIIAL ASYMGRATES LSIIAGPRIG
GLLNATFGNA VELIISLFAL KEGLTGIVLA SLTGSVLGNL LLVAGLSFFV GGLKYKRQEF
NIHDARHNSG LLIFAIIVAF VIPEVFSVGM GNASKLNLSI GISIIMILLY VAALYFKLVT
HRGVYQPNNA AQTEEEEEPE WSGKVATIVL FAATIVVAYI SENLVHTFHS VAEQFGWSEL
FIGVIIVAIV GNAAEHASAI IMAFKNKMDI AVEIAVGSTL QIAMFVAPVL VICSIFFPTS
MPLVFTLPEL VAMVSAVLLM IAISNDGDSN WFEGATLLAA YVIMAIGFFL L
