ID   CHAC2_XENLA             Reviewed;         184 AA.
AC   Q5PPV4;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   04-JAN-2005, sequence version 1.
DT   03-AUG-2022, entry version 59.
DE   RecName: Full=Putative glutathione-specific gamma-glutamylcyclotransferase 2 {ECO:0000250|UniProtKB:Q8WUX2};
DE            Short=Gamma-GCG 2 {ECO:0000250|UniProtKB:Q8WUX2};
DE            EC=4.3.2.7 {ECO:0000250|UniProtKB:Q8WUX2};
DE   AltName: Full=Cation transport regulator-like protein 2 {ECO:0000250|UniProtKB:Q9BUX1};
GN   Name=chac2 {ECO:0000250|UniProtKB:Q8WUX2};
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the cleavage of glutathione into 5-oxo-L-proline
CC       and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on
CC       other gamma-glutamyl peptides. {ECO:0000250|UniProtKB:Q8WUX2}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=glutathione = 5-oxo-L-proline + L-cysteinylglycine;
CC         Xref=Rhea:RHEA:47724, ChEBI:CHEBI:57925, ChEBI:CHEBI:58402,
CC         ChEBI:CHEBI:61694; EC=4.3.2.7;
CC         Evidence={ECO:0000250|UniProtKB:Q8WUX2};
CC   -!- SIMILARITY: Belongs to the gamma-glutamylcyclotransferase family. ChaC
CC       subfamily. {ECO:0000305}.
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DR   EMBL; BC087482; AAH87482.1; -; mRNA.
DR   RefSeq; NP_001088800.1; NM_001095331.1.
DR   AlphaFoldDB; Q5PPV4; -.
DR   SMR; Q5PPV4; -.
DR   DNASU; 496068; -.
DR   GeneID; 496068; -.
DR   KEGG; xla:496068; -.
DR   CTD; 496068; -.
DR   Xenbase; XB-GENE-5817366; chac2.S.
DR   OMA; LLWYTDF; -.
DR   OrthoDB; 1238273at2759; -.
DR   Proteomes; UP000186698; Chromosome 5S.
DR   Bgee; 496068; Expressed in oocyte and 19 other tissues.
DR   GO; GO:0003839; F:gamma-glutamylcyclotransferase activity; IEA:InterPro.
DR   GO; GO:0061928; F:glutathione specific gamma-glutamylcyclotransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006751; P:glutathione catabolic process; IEA:InterPro.
DR   CDD; cd06661; GGCT_like; 1.
DR   InterPro; IPR006840; ChaC.
DR   InterPro; IPR013024; GGCT-like.
DR   InterPro; IPR036568; GGCT-like_sf.
DR   PANTHER; PTHR12192; PTHR12192; 1.
DR   Pfam; PF04752; ChaC; 1.
DR   SUPFAM; SSF110857; SSF110857; 1.
PE   2: Evidence at transcript level;
KW   Lyase; Reference proteome.
FT   CHAIN           1..184
FT                   /note="Putative glutathione-specific gamma-
FT                   glutamylcyclotransferase 2"
FT                   /id="PRO_0000314917"
FT   ACT_SITE        83
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:O75223"
FT   BINDING         3..8
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:O75223"
SQ   SEQUENCE   184 AA;  21002 MW;  B691F82CF66DF39D CRC64;
     MWVFGYGSLI WKVDFPYVEK LVGYIMCYSR RFWQGSTDHR GVPGKPGRVV TLVEDPEGCV
     WGVAYRLPEG KEEEVKAYLD FREKGGYRTS TVVFYPKDPS IQPFNVLLYI GTCDNPNYLG
     PAPLEDIAEQ ILNAVGPSGR NTEYLFELAN SLRNLVPEDA DEHLFSLEKL VRQLSEAHRN
     LNHL