CHARK_ORYSJ
ID CHARK_ORYSJ Reviewed; 948 AA.
AC Q2QRN7; A0A0P0Y9Q4; Q0INH2; Q6PX60;
DT 16-SEP-2015, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Probable kinase CHARK {ECO:0000305};
DE EC=2.7.11.- {ECO:0000255|PROSITE-ProRule:PRU00159};
DE AltName: Full=CHASE domain receptor-like serine/threonine kinase {ECO:0000303|PubMed:16919402};
DE AltName: Full=OsCRL4 {ECO:0000305};
DE AltName: Full=OsHKL1 {ECO:0000303|PubMed:17408920};
GN Name=CHARK {ECO:0000303|PubMed:16919402};
GN OrderedLocusNames=Os12g0454800 {ECO:0000312|EMBL:BAF29743.1},
GN LOC_Os12g26940 {ECO:0000312|EMBL:ABA98351.1};
GN ORFNames=OsJ_36000 {ECO:0000312|EMBL:EAZ20394.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RC STRAIN=cv. Nipponbare;
RX PubMed=16919402; DOI=10.1016/j.gene.2006.06.020;
RA Ito Y., Kurata N.;
RT "Identification and characterization of cytokinin-signalling gene families
RT in rice.";
RL Gene 382:57-65(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Wu P., Han Q.M., Qi X.P.;
RT "Oryza sativa putative cytokinin receptor mRNA.";
RL Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16188032; DOI=10.1186/1741-7007-3-20;
RG The rice chromosomes 11 and 12 sequencing consortia;
RT "The sequence of rice chromosomes 11 and 12, rich in disease resistance
RT genes and recent gene duplications.";
RL BMC Biol. 3:20-20(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [8]
RP TISSUE SPECIFICITY.
RX PubMed=17408920; DOI=10.1016/j.ygeno.2007.02.001;
RA Du L., Jiao F., Chu J., Jin G., Chen M., Wu P.;
RT "The two-component signal system in rice (Oryza sativa L.): a genome-wide
RT study of cytokinin signal perception and transduction.";
RL Genomics 89:697-707(2007).
CC -!- FUNCTION: Putative receptor kinase that may be involved in cytokinin
CC signaling. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in roots (PubMed:16919402,
CC PubMed:17408920). Expressed in leaf blades, leaf sheaths, shoot apex,
CC flowers and panicles (PubMed:16919402). {ECO:0000269|PubMed:16919402,
CC ECO:0000269|PubMed:17408920}.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF29743.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB246779; BAE97710.1; -; Genomic_DNA.
DR EMBL; AY572461; AAS78641.1; -; mRNA.
DR EMBL; DP000011; ABA98351.1; -; Genomic_DNA.
DR EMBL; AP008218; BAF29743.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP014968; BAT17027.1; -; Genomic_DNA.
DR EMBL; CM000149; EAZ20394.1; -; Genomic_DNA.
DR RefSeq; XP_015620760.1; XM_015765274.1.
DR AlphaFoldDB; Q2QRN7; -.
DR SMR; Q2QRN7; -.
DR STRING; 4530.OS12T0454800-01; -.
DR PaxDb; Q2QRN7; -.
DR PRIDE; Q2QRN7; -.
DR EnsemblPlants; Os12t0454800-01; Os12t0454800-01; Os12g0454800.
DR GeneID; 4352167; -.
DR Gramene; Os12t0454800-01; Os12t0454800-01; Os12g0454800.
DR KEGG; osa:4352167; -.
DR eggNOG; KOG0519; Eukaryota.
DR HOGENOM; CLU_012295_0_0_1; -.
DR OMA; DTGHIRS; -.
DR OrthoDB; 684563at2759; -.
DR Proteomes; UP000000763; Chromosome 12.
DR Proteomes; UP000007752; Chromosome 12.
DR Proteomes; UP000059680; Chromosome 12.
DR Genevisible; Q2QRN7; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004675; F:transmembrane receptor protein serine/threonine kinase activity; IBA:GO_Central.
DR GO; GO:0009736; P:cytokinin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0042742; P:defense response to bacterium; IBA:GO_Central.
DR GO; GO:0002229; P:defense response to oomycetes; IBA:GO_Central.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR Gene3D; 3.30.450.350; -; 1.
DR InterPro; IPR006189; CHASE_dom.
DR InterPro; IPR042240; CHASE_sf.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR Pfam; PF03924; CHASE; 1.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM01079; CHASE; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS50839; CHASE; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Cell membrane; Cytokinin signaling pathway; Kinase; Membrane;
KW Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase;
KW Transferase; Transmembrane; Transmembrane helix;
KW Two-component regulatory system.
FT CHAIN 1..948
FT /note="Probable kinase CHARK"
FT /id="PRO_0000433812"
FT TOPO_DOM 1..290
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 291..311
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 312..437
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 438..458
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 459..601
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 602..622
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 623..948
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT DOMAIN 70..280
FT /note="CHASE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00049"
FT DOMAIN 660..948
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 570..596
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 802
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 666..674
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 689
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
SQ SEQUENCE 948 AA; 107228 MW; 0CFB9B4970700C36 CRC64;
MALLLWVFSC SLFSLWILSF MRSQATEARH GTLAVMCDER ARILQDQVKV SMNHLQALAI
LVSTFHHSKS PSAIDQTTFA RYVERTAFER PLTSGLAYAA RVTHSERELF ERQQAWSIRA
MNFSSKRPRA EEYAPVIFAQ DAYKHVVSID MLSGAEDRGN LLRARESGKV VLTAPFQLLN
KRIGVVLTYA VYKSELPLNA TVHDRIQSSI GYLGGVFDIE GHVDKLLEKL AGKEPMTVNI
YDTTGESMIR MYGSSNESAS GMCHVSTLDF GDPLRKHEMH CRFTQGPPWP WLAVASSYGT
LVISLLVGYI FHFTDKWIAK VEDGYKATDM QMPAKDEFAA TERISDMERD LKEDALFFDT
TKSPSLLEIS RLLNHRDPAQ NVHQEEQELN LPLEAQDKLK ETERKLGRMS KFITKVMKLT
SASIRCLPSR FHCFNKKVWS ISLLGFLLFI LVIGAFDQPY NQPLGMGGEG DNNMMLKNFG
FSRGRLLIDT LHGTWTKRGV QSSDTIRVDL RKMTRNNDSS GQQLKHWSSH KSSEIPAVLY
VPMNGKQVIV HCNLIVDEKA LVNWISNGDT DQSSKYQKET AGIQNRTDKP HLPANKSHKT
TVSPWIVLLP VIMLVLLGSI IWRRCNDHRR RVQQKELELL GIMGPSRFQL QDLVAATGNF
ADENKLGQGG FGPVYKGYLR DQDLHVAIKV LSRRQSCQEQ SAQGLREFKA EVKVMTQLRH
RNIVKLVGWS DSKKQLLLVY ELMAQGSLDK HLYDPEKILT WQQRYQIKFA NLFNSTDKIV
LDLGSALLYL HHDCEKCIVH GDIKPANVML DVSHNAKLGD FGLARLVEHG GEPQTTQVVA
GTPGYIDPEF INNRWPRTEL DVYSFGIVLL EIACGKRPAS RQLPNGASSL LAWVRDLYDQ
GKILDAADQR LNGEFNQQQM ERVIVMGLCC SHQDPIQRPS IVQAMDVL
