ID   CHAR_MOMCH              Reviewed;          25 AA.
AC   P84072;
DT   16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 32.
DE   RecName: Full=Ribosome-inactivating protein charantin;
DE            EC=3.2.2.22;
DE   AltName: Full=rRNA N-glycosidase;
DE   Flags: Fragment;
OS   Momordica charantia (Bitter gourd) (Balsam pear).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Momordiceae; Momordica.
OX   NCBI_TaxID=3673;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, AND FUNCTION.
RC   TISSUE=Seed {ECO:0000269|PubMed:11966976};
RX   PubMed=11966976; DOI=10.1034/j.1399-3011.2002.00978.x;
RA   Parkash A., Ng T.B., Tso W.W.;
RT   "Purification and characterization of charantin, a napin-like ribosome-
RT   inactivating peptide from bitter gourd (Momordica charantia) seeds.";
RL   J. Pept. Res. 59:197-202(2002).
CC   -!- FUNCTION: Inhibits cell-free translation in a rabbit reticulocyte
CC       lysate system. {ECO:0000269|PubMed:11966976}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of the N-glycosidic bond at one specific
CC         adenosine on the 28S rRNA.; EC=3.2.2.22;
CC         Evidence={ECO:0000269|PubMed:11966976};
CC   -!- SUBUNIT: Monomer. {ECO:0000305}.
CC   -!- MISCELLANEOUS: IC(50) of translation-inhibiting activity is of 400 nM.
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DR   AlphaFoldDB; P84072; -.
DR   Proteomes; UP000504603; Unplaced.
DR   GO; GO:0030598; F:rRNA N-glycosylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Glycosidase; Hydrolase;
KW   Protein synthesis inhibitor; Reference proteome; Toxin.
FT   CHAIN           1..>25
FT                   /note="Ribosome-inactivating protein charantin"
FT                   /id="PRO_0000221424"
FT   NON_TER         25
FT                   /evidence="ECO:0000303|PubMed:11966976"
SQ   SEQUENCE   25 AA;  2905 MW;  BFEBA068800E0696 CRC64;
     LRNVEQQCRA DALVERAQEL IHGQQ