CHAT1_CAEEL
ID CHAT1_CAEEL Reviewed; 348 AA.
AC H2L0H3; H2L0H4; Q2L6W0; U4PMW1;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2012, sequence version 1.
DT 03-AUG-2022, entry version 65.
DE RecName: Full=CDC50 family protein chat-1 {ECO:0000303|PubMed:21170358};
GN Name=chat-1 {ECO:0000312|WormBase:R08C7.2a};
GN ORFNames=R08C7.2 {ECO:0000312|WormBase:R08C7.2a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=21170358; DOI=10.1371/journal.pgen.1001235;
RA Chen B., Jiang Y., Zeng S., Yan J., Li X., Zhang Y., Zou W., Wang X.;
RT "Endocytic sorting and recycling require membrane phosphatidylserine
RT asymmetry maintained by TAT-1/CHAT-1.";
RL PLoS Genet. 6:E1001235-E1001235(2010).
CC -!- FUNCTION: Probable chaperone protein for the phospholipid-transporting
CC ATPase tat-1. Regulates cell membrane structure and function. Plays a
CC role in maintaining the membrane phosphatidylserine asymmetry and the
CC formation of the tubular membrane structure. Involved in membrane
CC trafficking and is specifically involved in the recycling and
CC degradation of endocytic cargo and this is likely with the
CC phospholipid-transporting ATPase tat-1. {ECO:0000269|PubMed:21170358}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:21170358};
CC Multi-pass membrane protein {ECO:0000255}. Early endosome membrane
CC {ECO:0000269|PubMed:21170358}; Multi-pass membrane protein. Recycling
CC endosome membrane {ECO:0000269|PubMed:21170358}; Multi-pass membrane
CC protein. Endoplasmic reticulum {ECO:0000269|PubMed:21170358}.
CC Note=Colocalizes with phospholipid-transporting ATPase tat-1 at apical
CC and basolateral cell membranes, on early endosomes and on recycling
CC endosomes. Also localizes to intracellular tubular and vesicular
CC structures. {ECO:0000269|PubMed:21170358}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=a {ECO:0000312|WormBase:R08C7.2a};
CC IsoId=H2L0H3-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:R08C7.2b};
CC IsoId=H2L0H3-2; Sequence=VSP_058022, VSP_058023;
CC Name=c {ECO:0000312|WormBase:R08C7.2c};
CC IsoId=H2L0H3-3; Sequence=VSP_058021;
CC Name=d {ECO:0000312|WormBase:R08C7.2d};
CC IsoId=H2L0H3-4; Sequence=VSP_058020;
CC -!- DEVELOPMENTAL STAGE: Expressed from early embryogenesis to adulthood in
CC various cell types. {ECO:0000269|PubMed:21170358}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in the appearance
CC of abnormal vacuoles in the intestines and defective recycling of
CC endocytic cargo with the accumulation of cargo within abnormal early
CC endosomes. {ECO:0000269|PubMed:21170358}.
CC -!- SIMILARITY: Belongs to the CDC50/LEM3 family. {ECO:0000305}.
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DR EMBL; BX284604; CCD73083.1; -; Genomic_DNA.
DR EMBL; BX284604; CCD73084.1; -; Genomic_DNA.
DR EMBL; BX284604; CCD73085.1; -; Genomic_DNA.
DR EMBL; BX284604; CDH93430.1; -; Genomic_DNA.
DR RefSeq; NP_001023332.1; NM_001028161.3. [H2L0H3-1]
DR RefSeq; NP_001040979.1; NM_001047514.2. [H2L0H3-3]
DR RefSeq; NP_001294623.1; NM_001307694.1.
DR AlphaFoldDB; H2L0H3; -.
DR SMR; H2L0H3; -.
DR STRING; 6239.R08C7.2a; -.
DR TCDB; 8.A.27.1.8; the cdc50 p-type atpase lipid flippase subunit (cdc50) family.
DR EPD; H2L0H3; -.
DR PaxDb; H2L0H3; -.
DR PeptideAtlas; H2L0H3; -.
DR EnsemblMetazoa; R08C7.2a.1; R08C7.2a.1; WBGene00019946. [H2L0H3-1]
DR EnsemblMetazoa; R08C7.2c.1; R08C7.2c.1; WBGene00019946. [H2L0H3-3]
DR EnsemblMetazoa; R08C7.2d.1; R08C7.2d.1; WBGene00019946. [H2L0H3-4]
DR GeneID; 177213; -.
DR KEGG; cel:CELE_R08C7.2; -.
DR UCSC; R08C7.2a; c. elegans.
DR CTD; 177213; -.
DR WormBase; R08C7.2a; CE07425; WBGene00019946; chat-1. [H2L0H3-1]
DR WormBase; R08C7.2b; CE33681; WBGene00019946; chat-1. [H2L0H3-2]
DR WormBase; R08C7.2c; CE39610; WBGene00019946; chat-1. [H2L0H3-3]
DR WormBase; R08C7.2d; CE48798; WBGene00019946; chat-1. [H2L0H3-4]
DR eggNOG; KOG2952; Eukaryota.
DR GeneTree; ENSGT00390000004660; -.
DR HOGENOM; CLU_025025_1_0_1; -.
DR InParanoid; H2L0H3; -.
DR OMA; TWNNDQP; -.
DR OrthoDB; 889671at2759; -.
DR PhylomeDB; H2L0H3; -.
DR Reactome; R-CEL-6798695; Neutrophil degranulation.
DR PRO; PR:H2L0H3; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00019946; Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues.
DR GO; GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0055038; C:recycling endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0033036; P:macromolecule localization; IEA:UniProt.
DR InterPro; IPR005045; CDC50/LEM3_fam.
DR PANTHER; PTHR10926; PTHR10926; 1.
DR Pfam; PF03381; CDC50; 1.
DR PIRSF; PIRSF015840; DUF284_TM_euk; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Endoplasmic reticulum; Endosome;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..348
FT /note="CDC50 family protein chat-1"
FT /evidence="ECO:0000305"
FT /id="PRO_0000435142"
FT TRANSMEM 53..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 309..329
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..241
FT /note="Missing (in isoform d)"
FT /evidence="ECO:0000305"
FT /id="VSP_058020"
FT VAR_SEQ 1..181
FT /note="Missing (in isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_058021"
FT VAR_SEQ 216..219
FT /note="NTTK -> VLTS (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_058022"
FT VAR_SEQ 220..348
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_058023"
SQ SEQUENCE 348 AA; 38572 MW; A1BB018706407492 CRC64;
MPPRDAVPTS TQVSGIGADG VQTEKVLKNR PKASALRQQK LPAWQPILTA TTVIPTVFVI
GAIFLPIGVF LFIASDAVSE FTVEYTNCLS PCQLQINLPN AFDGDVFLYY NLENYYQNHR
RYVKSRNDQQ YLGDLTNVKD CAPFDIDPAT KKPIAPCGAI ANSIFNDTFT LAHRADTGIV
TMVPVTTQGV IWNVDKDRKF KNPPLNDGNL CDAFNNTTKP PNWSKNPCEV GGFENVDFIV
WMRTAALPYF KKLWRIVDRT TNPLFSNGLP QGTYILTVEN NYPVQSFGGK KEFVISTTSW
AGGKNSFLGI AYLVVGSLAI VLGVVFIVIH MKFGHSMNEL SNVSEIHH
