ID   22P2_RAT                Reviewed;         176 AA.
AC   P22283; Q497B4;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=Cystatin-related protein 2;
DE   AltName: Full=Prostatic 22 kDa glycoprotein P22K15;
DE   Flags: Precursor;
GN   Name=Crp2; Synonyms=P22k15;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Wistar; TISSUE=Prostate;
RX   PubMed=2280780; DOI=10.1210/mend-4-4-657;
RA   Winderickx J., Hemschoote K., de Clercq N., van Dijck P., Peeters B.,
RA   Rombauts W., Verhoeven G., Heyns W.;
RT   "Tissue-specific expression and androgen regulation of different genes
RT   encoding rat prostatic 22-kilodalton glycoproteins homologous to human and
RT   rat cystatin.";
RL   Mol. Endocrinol. 4:657-667(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=Wistar;
RX   PubMed=7679983; DOI=10.1210/endo.132.3.7679983;
RA   Vercaeren I., Winderickx J., Devos A., Peeters B., Heyns W.;
RT   "An effect of androgens on the length of the poly(A)-tail and alternative
RT   splicing cause size heterogeneity of the messenger ribonucleic acids
RT   encoding cystatin-related protein.";
RL   Endocrinology 131:2496-2502(1992).
RN   [3]
RP   ERRATUM OF PUBMED:7679983.
RA   Vercaeren I., Winderickx J., Devos A., Peeters B., Heyns W.;
RL   Endocrinology 132:2496-2502(1993).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8462870; DOI=10.1016/0378-1119(93)90323-u;
RA   Devos A., de Clercq N., Vercaeren I., Heyns W., Rombauts W., Peeters B.;
RT   "Structure of rat genes encoding androgen-regulated cystatin-related
RT   proteins (CRPs): a new member of the cystatin superfamily.";
RL   Gene 125:159-167(1993).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Prostate;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- TISSUE SPECIFICITY: Prostate.
CC   -!- INDUCTION: By androgens.
CC   -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000305}.
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DR   EMBL; M58169; AAA63499.1; -; mRNA.
DR   EMBL; Z13994; CAA78385.1; -; Genomic_DNA.
DR   EMBL; BC100632; AAI00633.1; -; mRNA.
DR   PIR; JT0616; JT0616.
DR   RefSeq; NP_954887.1; NM_199266.2.
DR   AlphaFoldDB; P22283; -.
DR   STRING; 10116.ENSRNOP00000043921; -.
DR   GlyGen; P22283; 1 site.
DR   PaxDb; P22283; -.
DR   Ensembl; ENSRNOT00000050369; ENSRNOP00000043921; ENSRNOG00000030184.
DR   GeneID; 296229; -.
DR   KEGG; rno:296229; -.
DR   UCSC; RGD:727814; rat.
DR   CTD; 296229; -.
DR   RGD; 727814; P22k15.
DR   InParanoid; P22283; -.
DR   OrthoDB; 1565344at2759; -.
DR   PhylomeDB; P22283; -.
DR   TreeFam; TF340321; -.
DR   PRO; PR:P22283; -.
DR   Proteomes; UP000002494; Chromosome 3.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0031982; C:vesicle; IBA:GO_Central.
DR   GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IBA:GO_Central.
DR   GO; GO:2000117; P:negative regulation of cysteine-type endopeptidase activity; IBA:GO_Central.
DR   CDD; cd00042; CY; 1.
DR   InterPro; IPR000010; Cystatin_dom.
DR   InterPro; IPR046350; Cystatin_sf.
DR   SUPFAM; SSF54403; SSF54403; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Glycoprotein; Protease inhibitor; Reference proteome;
KW   Signal; Thiol protease inhibitor.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   PROPEP          27..30
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000006673"
FT   CHAIN           31..176
FT                   /note="Cystatin-related protein 2"
FT                   /id="PRO_0000006674"
FT   CARBOHYD        71
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000305"
FT   DISULFID        129..139
FT                   /evidence="ECO:0000250"
FT   DISULFID        153..173
FT                   /evidence="ECO:0000250"
FT   CONFLICT        149..176
FT                   /note="KREICYFQLYPDYIEQNIRSVRFNCYTK -> IERNMLLSTVS (in Ref.
FT                   4; CAA78385)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   176 AA;  21013 MW;  1B8F4FAEBA06B461 CRC64;
     MYKTLCGTQL LLAIFVLFLN FSHATAKGTR GPMEIFKKNF MEKNKLNDVY DIFKFLYNKF
     SHDTYLSNIK NQSFTMNTWG FGEIEVVKTK CRKIDSDFYK CSFQWEFCNI KRTPGVTIYY
     ITLPGSVRCR KLLSKLVNCP FEEQTEQLKR EICYFQLYPD YIEQNIRSVR FNCYTK