CHE1_CAEEL
ID CHE1_CAEEL Reviewed; 279 AA.
AC Q966L8;
DT 16-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT 16-FEB-2004, sequence version 3.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Transcription factor che-1;
DE AltName: Full=Abnormal chemotaxis protein 1;
GN Name=che-1; ORFNames=C55B7.12;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM B), FUNCTION, TISSUE
RP SPECIFICITY, AND MUTAGENESIS OF PRO-255; CYS-261; GLY-269 AND HIS-274.
RC STRAIN=Bristol N2;
RX PubMed=12588839; DOI=10.1242/dev.00341;
RA Uchida O., Nakano H., Koga M., Ohshima Y.;
RT "The C. elegans che-1 gene encodes a zinc finger transcription factor
RT required for specification of the ASE chemosensory neurons.";
RL Development 130:1215-1224(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A), FUNCTION, AND MUTANTS OT63
RP AND OT70.
RX PubMed=12952888; DOI=10.1101/gad.1117903;
RA Chang S., Johnston R.J. Jr., Hobert O.;
RT "A transcriptional regulatory cascade that controls left/right asymmetry in
RT chemosensory neurons of C. elegans.";
RL Genes Dev. 17:2123-2137(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: Transcription factor essential for the identity of ASE
CC chemosensory neurons. Acts by activating expression of genes that are
CC specific for ASE chemosensory neurons, such as seven-transmembrane
CC receptors or guanylate cyclase. Also required to control the left/right
CC asymmetry in ASE chemosensory neurons by regulating expression of genes
CC such as cog-1 and ceh-36. {ECO:0000269|PubMed:12588839,
CC ECO:0000269|PubMed:12952888}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a;
CC IsoId=Q966L8-1; Sequence=Displayed;
CC Name=b;
CC IsoId=Q966L8-2; Sequence=VSP_009389;
CC -!- TISSUE SPECIFICITY: Expressed in ASE chemosensory neurons and in some
CC other neurons. {ECO:0000269|PubMed:12588839}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CCD68097.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; FO080958; CCD68097.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; NP_001076597.1; NM_001083128.1.
DR AlphaFoldDB; Q966L8; -.
DR SMR; Q966L8; -.
DR STRING; 6239.C55B7.12b; -.
DR PaxDb; Q966L8; -.
DR EnsemblMetazoa; C55B7.12a.1; C55B7.12a.1; WBGene00000483.
DR GeneID; 183847; -.
DR KEGG; cel:CELE_C55B7.12; -.
DR UCSC; C55B7.12b; c. elegans. [Q966L8-1]
DR CTD; 183847; -.
DR WormBase; C55B7.12a; CE34280; WBGene00000483; che-1.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT01050000244908; -.
DR InParanoid; Q966L8; -.
DR OMA; MSYHYQM; -.
DR PRO; PR:Q966L8; -.
DR Proteomes; UP000001940; Chromosome I.
DR ExpressionAtlas; Q966L8; baseline.
DR GO; GO:0005634; C:nucleus; ISS:WormBase.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:WormBase.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:WormBase.
DR GO; GO:0007635; P:chemosensory behavior; IMP:UniProtKB.
DR GO; GO:0006935; P:chemotaxis; IMP:UniProtKB.
DR GO; GO:0030182; P:neuron differentiation; IMP:WormBase.
DR GO; GO:0050921; P:positive regulation of chemotaxis; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0050920; P:regulation of chemotaxis; IMP:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0010446; P:response to alkaline pH; IMP:UniProtKB.
DR GO; GO:0010035; P:response to inorganic substance; IMP:UniProtKB.
DR GO; GO:0010226; P:response to lithium ion; IMP:UniProtKB.
DR GO; GO:0032026; P:response to magnesium ion; IMP:UniProtKB.
DR GO; GO:1904640; P:response to methionine; IMP:UniProtKB.
DR GO; GO:1902074; P:response to salt; IMP:UniProtKB.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 4.
DR SMART; SM00355; ZnF_C2H2; 4.
DR SUPFAM; SSF57667; SSF57667; 3.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 4.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 4.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Chemotaxis; DNA-binding; Metal-binding;
KW Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..279
FT /note="Transcription factor che-1"
FT /id="PRO_0000046887"
FT ZN_FING 172..194
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 200..222
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 228..250
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 256..278
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT VAR_SEQ 70..75
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_009389"
FT MUTAGEN 255
FT /note="P->L: In ot70; leads to a loss of expression of lim-
FT 6 and cgy-7 in left ASE chemosensory neurons."
FT /evidence="ECO:0000269|PubMed:12588839"
FT MUTAGEN 261
FT /note="C->Y: In ot63; leads to a loss of expression of lim-
FT 6 and cgy-7 in left ASE chemosensory neurons."
FT /evidence="ECO:0000269|PubMed:12588839"
FT MUTAGEN 269
FT /note="G->R: In p692 and p696; induces defects in
FT chemotaxis to NaCl."
FT /evidence="ECO:0000269|PubMed:12588839"
FT MUTAGEN 274
FT /note="H->P: In e1034; induces defects in chemotaxis to
FT NaCl."
FT /evidence="ECO:0000269|PubMed:12588839"
SQ SEQUENCE 279 AA; 31595 MW; 62BDBBA565DE69B6 CRC64;
MSYHYQMNNN NTDFFDSANY IPQMQMHHGY QDFYASDSTS SMPSSGTTQQ ALQQTENGII
QGQNGSDHGV AFHFRAYGGW RQHDWNSTSS WSIGNQLEME NGTLTMSHGA TNDGNIRNNQ
QMMTSNHITT DSFRQIPPSG LCYMDPTPSL RLPKKEPLPV QRPMARSTPK PFRCQTCGKA
FSQAANLTAH KRIHTGEKPF MCPVCNRPFS QSSSLVTHRR THTGERPYPC AQCEKAFTDS
STLTKHLRTH TGHKPYVCSI CMMKFTQSGN LHRHMKTHK
