ID   CHEW_BACSU              Reviewed;         156 AA.
AC   P39802;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Chemotaxis protein CheW;
DE   AltName: Full=Coupling protein CheW;
GN   Name=cheW; OrderedLocusNames=BSU16440;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN CHEMOTAXIS, AND DISRUPTION
RP   PHENOTYPE.
RC   STRAIN=168 / OI1085;
RX   PubMed=1601874; DOI=10.1016/s0021-9258(19)49805-0;
RA   Hanlon D.W., Marquez-Magana L.M., Carpenter P.B., Chamberlin M.J.,
RA   Ordal G.W.;
RT   "Sequence and characterization of Bacillus subtilis CheW.";
RL   J. Biol. Chem. 267:12055-12060(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=168 / OI1085;
RX   PubMed=8169224; DOI=10.1128/jb.176.9.2736-2739.1994;
RA   Rosario M.M.L., Fredrick K.L., Ordal G.W., Helmann J.D.;
RT   "Chemotaxis in Bacillus subtilis requires either of two functionally
RT   redundant CheW homologs.";
RL   J. Bacteriol. 176:2736-2739(1994).
CC   -!- FUNCTION: Involved in the transmission of sensory signals from the
CC       chemoreceptors to the flagellar motors. CheV and CheW are involved in
CC       the coupling of the methyl-accepting chemoreceptors to the central two-
CC       component kinase CheA; they are both necessary for efficient
CC       chemotaxis. {ECO:0000269|PubMed:1601874, ECO:0000269|PubMed:8169224}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- DISRUPTION PHENOTYPE: Chemotaxis is substantially impaired, with a 30-
CC       fold reduction in the efficiency of chemotaxis with aspartate and 11-
CC       fold reduction with 2-deoxyglucose. Inactivation of cheW appears to
CC       have a subtle effect on chemotactic behavior in the absence of
CC       chemoeffectors: the bacteria display a smooth swimming bias, with some
CC       tumbling. A double mutant lacking both CheV and CheW has a strong
CC       tumble bias, does not respond to addition of attractant, shows
CC       essentially no accumulation in capillary assays, and has greatly
CC       reduced methyl turnover on the methyl-accepting chemotaxis proteins
CC       (MCPs). {ECO:0000269|PubMed:1601874, ECO:0000269|PubMed:8169224}.
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DR   EMBL; S38086; AAB22364.2; -; Genomic_DNA.
DR   EMBL; AL009126; CAB13517.1; -; Genomic_DNA.
DR   PIR; C69599; C69599.
DR   RefSeq; NP_389526.1; NC_000964.3.
DR   RefSeq; WP_003231938.1; NZ_JNCM01000035.1.
DR   AlphaFoldDB; P39802; -.
DR   SMR; P39802; -.
DR   STRING; 224308.BSU16440; -.
DR   jPOST; P39802; -.
DR   PaxDb; P39802; -.
DR   PRIDE; P39802; -.
DR   EnsemblBacteria; CAB13517; CAB13517; BSU_16440.
DR   GeneID; 939957; -.
DR   KEGG; bsu:BSU16440; -.
DR   PATRIC; fig|224308.179.peg.1785; -.
DR   eggNOG; COG0835; Bacteria.
DR   InParanoid; P39802; -.
DR   OMA; CVNIMSV; -.
DR   PhylomeDB; P39802; -.
DR   BioCyc; BSUB:BSU16440-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0006935; P:chemotaxis; IGI:CACAO.
DR   GO; GO:0042333; P:chemotaxis to oxidizable substrate; IMP:CACAO.
DR   GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR   InterPro; IPR039315; CheW.
DR   InterPro; IPR036061; CheW-like_dom_sf.
DR   InterPro; IPR002545; CheW-lke_dom.
DR   PANTHER; PTHR22617; PTHR22617; 1.
DR   Pfam; PF01584; CheW; 1.
DR   SMART; SM00260; CheW; 1.
DR   SUPFAM; SSF50341; SSF50341; 1.
DR   PROSITE; PS50851; CHEW; 1.
PE   1: Evidence at protein level;
KW   Chemotaxis; Cytoplasm; Reference proteome.
FT   CHAIN           1..156
FT                   /note="Chemotaxis protein CheW"
FT                   /id="PRO_0000198339"
FT   DOMAIN          8..148
FT                   /note="CheW-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00052"
SQ   SEQUENCE   156 AA;  17505 MW;  D7A253C6A70B64D7 CRC64;
     MTAEIKTGEK MIVFMVNGKE YAISVTQVKS IEKWQKPTRV PGVEPYICGV INLRGVVTPV
     IDLRKRLNLP EYEITDETRI IIIAYRDIEV GWIVDEANDV ITVHESEIES APEGVQKDTD
     VSIEQIVKQE NRLLNIIDAN AVLDKESSQS AVPDQA