ID   CHEY_HALS3              Reviewed;         120 AA.
AC   B0R4K1; Q48299; Q7LY97;
DT   14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT   08-APR-2008, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Chemotaxis protein CheY;
DE   AltName: Full=Response regulator CheY;
GN   Name=cheY; OrderedLocusNames=OE_2417R;
OS   Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC   Halobacteriaceae; Halobacterium.
OX   NCBI_TaxID=478009;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-6, FUNCTION,
RP   PHOSPHORYLATION AT ASP-53 BY CHEA, AND MUTAGENESIS OF ASP-53.
RC   STRAIN=R1 / S9 / D2;
RX   PubMed=7556066; DOI=10.1002/j.1460-2075.1995.tb00099.x;
RA   Rudolph J., Tolliday N., Schmitt C., Schuster S.C., Oesterhelt D.;
RT   "Phosphorylation in halobacterial signal transduction.";
RL   EMBO J. 14:4249-4257(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29341 / DSM 671 / R1;
RX   PubMed=18313895; DOI=10.1016/j.ygeno.2008.01.001;
RA   Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K.,
RA   Ruepp A., Soppa J., Tittor J., Oesterhelt D.;
RT   "Evolution in the laboratory: the genome of Halobacterium salinarum strain
RT   R1 compared to that of strain NRC-1.";
RL   Genomics 91:335-346(2008).
RN   [3]
RP   DISRUPTION PHENOTYPE.
RX   PubMed=8636990; DOI=10.1006/jmbi.1996.0267;
RA   Rudolph J., Oesterhelt D.;
RT   "Deletion analysis of the che operon in the archaeon Halobacterium
RT   salinarium.";
RL   J. Mol. Biol. 258:548-554(1996).
RN   [4]
RP   INTERACTION WITH CHEA.
RC   STRAIN=ATCC 29341 / DSM 671 / R1;
RX   PubMed=23171228; DOI=10.1186/1471-2180-12-272;
RA   Schlesner M., Miller A., Besir H., Aivaliotis M., Streif J., Scheffer B.,
RA   Siedler F., Oesterhelt D.;
RT   "The protein interaction network of a taxis signal transduction system in a
RT   halophilic archaeon.";
RL   BMC Microbiol. 12:272-272(2012).
CC   -!- FUNCTION: Involved in the transmission of sensory signals from the
CC       chemoreceptors and photoreceptors to the flagellar motors.
CC       {ECO:0000269|PubMed:7556066}.
CC   -!- SUBUNIT: Interacts with CheA. {ECO:0000269|PubMed:23171228}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- PTM: Phosphorylated by CheA. {ECO:0000269|PubMed:7556066}.
CC   -!- DISRUPTION PHENOTYPE: Deletion leads to the loss of both chemotactic
CC       and phototactic responses. {ECO:0000269|PubMed:8636990}.
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DR   EMBL; X86407; CAA60161.1; -; Genomic_DNA.
DR   EMBL; AM774415; CAP13666.1; -; Genomic_DNA.
DR   PIR; G84253; G84253.
DR   PIR; S58645; S58645.
DR   RefSeq; WP_010902692.1; NC_010364.1.
DR   AlphaFoldDB; B0R4K1; -.
DR   SMR; B0R4K1; -.
DR   EnsemblBacteria; CAP13666; CAP13666; OE_2417R.
DR   GeneID; 5953632; -.
DR   GeneID; 62886522; -.
DR   KEGG; hsl:OE_2417R; -.
DR   HOGENOM; CLU_000445_69_15_2; -.
DR   OMA; AAGAHEY; -.
DR   PhylomeDB; B0R4K1; -.
DR   Proteomes; UP000001321; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0006935; P:chemotaxis; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   Pfam; PF00072; Response_reg; 1.
DR   SMART; SM00448; REC; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   1: Evidence at protein level;
KW   Chemotaxis; Cytoplasm; Direct protein sequencing; Phosphoprotein;
KW   Two-component regulatory system.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:7556066"
FT   CHAIN           2..120
FT                   /note="Chemotaxis protein CheY"
FT                   /id="PRO_0000429067"
FT   DOMAIN          4..118
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   MOD_RES         53
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000305|PubMed:7556066"
FT   MUTAGEN         53
FT                   /note="D->A: Does not accept phosphate group from CheA."
FT                   /evidence="ECO:0000269|PubMed:7556066"
SQ   SEQUENCE   120 AA;  13436 MW;  76CB81AD6B17546B CRC64;
     MAKQVLLVDD SEFMRNLLRE ILEEEFEIAD EAENGVEAVE MYKEYDPDLV MMDIVMPIRD
     GIEATSEIKE FDAGAHIIMC TSIGQEEKMK KAVKAGADGY ITKPFQKPSV MDAISDVLTA