ID   CHH_PROBO               Reviewed;          72 AA.
AC   P55845;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Crustacean hyperglycemic hormone;
DE            Short=CHH;
OS   Procambarus bouvieri (Mexican crayfish).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Multicrustacea;
OC   Malacostraca; Eumalacostraca; Eucarida; Decapoda; Pleocyemata; Astacidea;
OC   Astacoidea; Cambaridae; Procambarus.
OX   NCBI_TaxID=6729;
RN   [1]
RP   PROTEIN SEQUENCE (CHH-I).
RC   TISSUE=Sinus gland;
RX   PubMed=8441709; DOI=10.1016/0196-9781(93)90004-z;
RA   Huberman A., Aguilar M.B., Brew K., Shabanowitz J., Hunt D.F.;
RT   "Primary structure of the major isomorph of the crustacean hyperglycemic
RT   hormone (CHH-I) from the sinus gland of the Mexican crayfish Procambarus
RT   bouvieri (Ortmann): interspecies comparison.";
RL   Peptides 14:7-16(1993).
RN   [2]
RP   PROTEIN SEQUENCE (CHH-II), MASS SPECTROMETRY, PYROGLUTAMATE FORMATION AT
RP   GLN-1, D-AMINO ACID AT PHE-3, AMIDATION AT VAL-72, AND DISULFIDE BONDS.
RC   TISSUE=Sinus gland;
RX   PubMed=8745046; DOI=10.1016/0196-9781(95)02024-1;
RA   Aguilar M.B., Soyez D., Falchetto R., Arnott D., Shabanowitz J., Hunt D.F.,
RA   Huberman A.;
RT   "Amino acid sequence of the minor isomorph of the crustacean hyperglycemic
RT   hormone (CHH-II) of the Mexican crayfish Procambarus bouvieri (Ortmann):
RT   presence of a D-amino acid.";
RL   Peptides 16:1375-1383(1995).
CC   -!- FUNCTION: Hormone found in the sinus gland of isopods and decapods
CC       which controls the blood sugar level. Has a secretagogue action over
CC       the amylase released from the midgut gland. May act as a stress hormone
CC       and may be involved in the control of molting and reproduction.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Produced by the medulla terminalis X-organ in the
CC       eyestalks and transported to the sinus gland where they are stored and
CC       released.
CC   -!- PTM: Stereoinversion of L-Phe (in CHH-I) to D-Phe (in CHH-II) the two
CC       forms are present in a ratio 3:1 (CHH-I/CHH-II).
CC       {ECO:0000269|PubMed:8745046}.
CC   -!- MASS SPECTROMETRY: Mass=8390; Mass_error=3; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:8745046};
CC   -!- SIMILARITY: Belongs to the arthropod CHH/MIH/GIH/VIH hormone family.
CC       {ECO:0000305}.
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DR   AlphaFoldDB; P55845; -.
DR   SMR; P55845; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005184; F:neuropeptide hormone activity; IEA:InterPro.
DR   GO; GO:0006006; P:glucose metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.2010.10; -; 1.
DR   InterPro; IPR018251; Crust_neurhormone_CS.
DR   InterPro; IPR031098; Crust_neurohorm.
DR   InterPro; IPR035957; Crust_neurohorm_sf.
DR   InterPro; IPR001166; Hyperglycemic.
DR   InterPro; IPR000346; Hyperglycemic1.
DR   PANTHER; PTHR35981; PTHR35981; 1.
DR   Pfam; PF01147; Crust_neurohorm; 1.
DR   PRINTS; PR00548; HYPRGLYCEMC1.
DR   PRINTS; PR00550; HYPRGLYCEMIC.
DR   SUPFAM; SSF81778; SSF81778; 1.
DR   PROSITE; PS01250; CHH_MIH_GIH; 1.
PE   1: Evidence at protein level;
KW   Amidation; Carbohydrate metabolism; D-amino acid;
KW   Direct protein sequencing; Disulfide bond; Glucose metabolism; Hormone;
KW   Neuropeptide; Pyrrolidone carboxylic acid; Secreted.
FT   CHAIN           1..72
FT                   /note="Crustacean hyperglycemic hormone"
FT                   /id="PRO_0000209861"
FT   MOD_RES         1
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:8745046"
FT   MOD_RES         3
FT                   /note="D-phenylalanine; in form CHH-II"
FT                   /evidence="ECO:0000269|PubMed:8745046"
FT   MOD_RES         72
FT                   /note="Valine amide"
FT                   /evidence="ECO:0000269|PubMed:8745046"
FT   DISULFID        7..43
FT                   /evidence="ECO:0000269|PubMed:8745046"
FT   DISULFID        23..39
FT                   /evidence="ECO:0000269|PubMed:8745046"
FT   DISULFID        26..52
FT                   /evidence="ECO:0000269|PubMed:8745046"
SQ   SEQUENCE   72 AA;  8411 MW;  D7EFA84530AD4DE3 CRC64;
     QVFDQACKGI YDRAIFKKLD RVCEDCYNLY RKPYVATTCR QNCYANSVFR QCLDDLLLID
     VVDEYISGVQ TV