ID   CHI1_STROI              Reviewed;          74 AA.
AC   P29115;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   11-DEC-2019, entry version 74.
DE   RecName: Full=Chitinases 70, 30, and 20.5 kDa;
DE            EC=3.2.1.14;
DE   Flags: Fragments;
OS   Streptomyces olivaceoviridis (Streptomyces corchorusii).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1921;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   STRAIN=ATCC 11238 / DSM 41433 / NCIB 8592 / QM B814;
RX   PubMed=1592803; DOI=10.1128/jb.174.11.3450-3454.1992;
RA   Romaguera A., Menge U., Breves R., Diekmann H.;
RT   "Chitinases of Streptomyces olivaceoviridis and significance of processing
RT   for multiplicity.";
RL   J. Bacteriol. 174:3450-3454(1992).
CC   -!- FUNCTION: Able to cleave chitin oligomers from N=3 to 6.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide
CC         (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14;
CC   -!- SUBUNIT: Homodimer, but homotrimers and homotetramers could be observed
CC       for the 20.5 and 30 kDa chitinases.
CC   -!- PTM: The 70 kDa chitinase is probably the precursor protein of the 30
CC       and 20.5 kDa chitinases.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 18 family. Chitinase
CC       class II subfamily. {ECO:0000305}.
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DR   PIR; A44908; A44908.
DR   PIR; B44908; B44908.
DR   PIR; D44908; D44908.
DR   GO; GO:0004568; F:chitinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006032; P:chitin catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Carbohydrate metabolism; Chitin degradation; Direct protein sequencing;
KW   Glycosidase; Hydrolase; Polysaccharide degradation.
FT   CHAIN           1..>74
FT                   /note="Chitinases 70, 30, and 20.5 kDa"
FT                   /id="PRO_0000077045"
FT   REGION          1..27
FT                   /note="N-terminus of 70 kDa chitinase"
FT   REGION          28..52
FT                   /note="N-terminus of 30 kDa chitinase"
FT   REGION          53..74
FT                   /note="N-terminus of 20.5 kDa chitinase"
FT   NON_CONS        27..28
FT                   /evidence="ECO:0000305"
FT   NON_CONS        52..53
FT                   /evidence="ECO:0000305"
FT   NON_TER         74
SQ   SEQUENCE   74 AA;  8102 MW;  33084754CC5E7552 CRC64;
     XTSATATYAK TQDWGSCFEG KWTIKNTAAC SSYPSWVAGR SYAAGDIVYY TDXGYTDLPV
     SRQKMCQNGM VTNC