ID   CHI2_GOSHI              Reviewed;         302 AA.
AC   Q39785;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 2.
DT   25-MAY-2022, entry version 104.
DE   RecName: Full=Endochitinase 2;
DE            EC=3.2.1.14;
DE   Flags: Precursor; Fragment;
OS   Gossypium hirsutum (Upland cotton) (Gossypium mexicanum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX   NCBI_TaxID=3635;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=cv. Stoneville 887; TISSUE=Leaf;
RA   Levorson J.P., Chlan C.A.;
RT   "Cloning of an ethylene-responsive chitinase from cotton.";
RL   (er) Plant Gene Register PGR97-034(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE OF 112-205.
RC   STRAIN=cv. Stoneville 887; TISSUE=Leaf;
RA   Levorson J.P., Chlan C.A.;
RL   Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Defense against chitin-containing fungal pathogens.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide
CC         (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14;
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 19 family. Chitinase
CC       class I subfamily. {ECO:0000305}.
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DR   EMBL; U78888; AAB68047.1; -; mRNA.
DR   EMBL; U57408; AAB01339.1; -; Genomic_DNA.
DR   PIR; T10810; T10810.
DR   AlphaFoldDB; Q39785; -.
DR   SMR; Q39785; -.
DR   CAZy; CBM18; Carbohydrate-Binding Module Family 18.
DR   CAZy; GH19; Glycoside Hydrolase Family 19.
DR   Proteomes; UP000189702; Genome assembly.
DR   GO; GO:0008061; F:chitin binding; IEA:UniProtKB-KW.
DR   GO; GO:0004568; F:chitinase activity; IBA:GO_Central.
DR   GO; GO:0016998; P:cell wall macromolecule catabolic process; IEA:InterPro.
DR   GO; GO:0006032; P:chitin catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IBA:GO_Central.
DR   GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.60.10; -; 1.
DR   InterPro; IPR001002; Chitin-bd_1.
DR   InterPro; IPR018371; Chitin-binding_1_CS.
DR   InterPro; IPR036861; Endochitinase-like_sf.
DR   InterPro; IPR016283; Glyco_hydro_19.
DR   InterPro; IPR000726; Glyco_hydro_19_cat.
DR   InterPro; IPR023346; Lysozyme-like_dom_sf.
DR   Pfam; PF00187; Chitin_bind_1; 1.
DR   Pfam; PF00182; Glyco_hydro_19; 1.
DR   PIRSF; PIRSF001060; Endochitinase; 1.
DR   PRINTS; PR00451; CHITINBINDNG.
DR   SMART; SM00270; ChtBD1; 1.
DR   SUPFAM; SSF53955; SSF53955; 1.
DR   SUPFAM; SSF57016; SSF57016; 1.
DR   PROSITE; PS00026; CHIT_BIND_I_1; 1.
DR   PROSITE; PS50941; CHIT_BIND_I_2; 1.
DR   PROSITE; PS00773; CHITINASE_19_1; 1.
DR   PROSITE; PS00774; CHITINASE_19_2; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Chitin degradation; Chitin-binding;
KW   Disulfide bond; Glycosidase; Hydrolase; Plant defense;
KW   Polysaccharide degradation; Reference proteome.
FT   CHAIN           1..295
FT                   /note="Endochitinase 2"
FT                   /id="PRO_0000005294"
FT   PROPEP          296..302
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000005295"
FT   DOMAIN          <1..42
FT                   /note="Chitin-binding type-1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00261"
FT   ACT_SITE        117
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P29022"
FT   DISULFID        3..18
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00261"
FT   DISULFID        12..24
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00261"
FT   DISULFID        17..31
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00261"
FT   DISULFID        36..40
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00261"
FT   DISULFID        73..136
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00261"
FT   DISULFID        148..156
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00261"
FT   DISULFID        255..287
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00261"
FT   NON_TER         1
SQ   SEQUENCE   302 AA;  32262 MW;  F6BD0C9AB6BC1870 CRC64;
     EQCGRQAGGA LCPGGLCCSQ FGWCGSTADY CTVPGCQSQC SGSGPAPGPG GLTNLISRET
     FNQMLLHRND GACPARGFYT YDAFIAAARS FPAFATTGDQ ATRKREIAAF LAQTSHETTG
     GAGWAAPDGP YAWGYCYNRE LNPPSSYCAS DPNYPCAPGK QYFGRGPMQL SWNYNYGQCG
     RAIGVDLLNN PDLLSSDPTI SFKSAFWFWM TPQSPKPSCH NVIIGAWSPS SSDRAAGRAT
     GYGVITNIIN GGLECGKGWN AQVEDRIGFY KRYCDILGVS YGNNLDCYNQ SPFGNGVSVD
     SM