ID   CHIA_SOLCI              Reviewed;         253 AA.
AC   Q40114; Q40113;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Acidic endochitinase pcht28;
DE            EC=3.2.1.14;
DE   Flags: Precursor;
OS   Solanum chilense (Tomato) (Lycopersicon chilense).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC   Solanum subgen. Lycopersicon.
OX   NCBI_TaxID=4083;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. LA1930; TISSUE=Leaf;
RX   PubMed=7816027; DOI=10.1007/bf00283267;
RA   Chen R.D., Yu L.X., Greer A.F., Cheriti H., Tabaeizadeh Z.;
RT   "Isolation of an osmotic stress- and abscisic acid-induced gene encoding an
RT   acidic endochitinase from Lycopersicon chilense.";
RL   Mol. Gen. Genet. 245:195-202(1994).
CC   -!- FUNCTION: Defense against chitin-containing fungal pathogens.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide
CC         (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14;
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space.
CC   -!- INDUCTION: By osmotic stress and abscisic acid (ABA).
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 19 family. Chitinase
CC       class II subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; L19342; AAA64999.1; -; mRNA.
DR   EMBL; M97210; AAA64998.1; -; mRNA.
DR   PIR; S51588; S51588.
DR   PIR; S51589; S51589.
DR   AlphaFoldDB; Q40114; -.
DR   SMR; Q40114; -.
DR   CAZy; GH19; Glycoside Hydrolase Family 19.
DR   GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR   GO; GO:0004568; F:chitinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016998; P:cell wall macromolecule catabolic process; IEA:InterPro.
DR   GO; GO:0006032; P:chitin catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR016283; Glyco_hydro_19.
DR   InterPro; IPR000726; Glyco_hydro_19_cat.
DR   InterPro; IPR023346; Lysozyme-like_dom_sf.
DR   Pfam; PF00182; Glyco_hydro_19; 1.
DR   PIRSF; PIRSF001060; Endochitinase; 1.
DR   SUPFAM; SSF53955; SSF53955; 1.
DR   PROSITE; PS00773; CHITINASE_19_1; 1.
DR   PROSITE; PS00774; CHITINASE_19_2; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Chitin degradation; Disulfide bond; Glycosidase;
KW   Hydrolase; Plant defense; Polysaccharide degradation; Secreted; Signal;
KW   Stress response.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000250"
FT   CHAIN           25..253
FT                   /note="Acidic endochitinase pcht28"
FT                   /id="PRO_0000005298"
FT   ACT_SITE        92
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P29022"
FT   DISULFID        212..244
FT                   /evidence="ECO:0000250"
FT   CONFLICT        94
FT                   /note="K -> N (in Ref. 1; AAA64998)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        160
FT                   /note="R -> K (in Ref. 1; AAA64998)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        170..171
FT                   /note="QD -> HH (in Ref. 1; AAA64998)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   253 AA;  27569 MW;  D48C269D5A794E60 CRC64;
     MKFNIVSPVA LSCLFFLFLT GTLAQNAGSI VTRELFEQML SFRNNDACPA KGFYTYDAFI
     AAANSFPGFG TTGDDTARKK EIAAFFGQTS HETKGGSAGT FTGGYCFVRQ IDQSDRYYGR
     GPIQLTHQSN YERAGQGIGV GQDLVNNPDL VATDPIISFR TAIWFWMTAQ DNKPSCHNVI
     IGQWTPSPAD TAANRVPGYG VITNIINGGL ECNMGPNTAV ESRIGFYRRY CGMLNVPTGE
     NLDCNNQKNF AQG