CHIA_SOLLC
ID CHIA_SOLLC Reviewed; 253 AA.
AC Q05539;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Acidic 26 kDa endochitinase;
DE EC=3.2.1.14;
DE Flags: Precursor;
GN Name=CHI3;
OS Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC Solanum subgen. Lycopersicon.
OX NCBI_TaxID=4081;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 134-152 AND 225-228.
RC STRAIN=cv. Moneymaker;
RX PubMed=8400122; DOI=10.1007/bf00028974;
RA Danhash N., Wagemakers C.A.M., van Kan J.A.L., de Wit P.J.G.M.;
RT "Molecular characterization of four chitinase cDNAs obtained from
RT Cladosporium fulvum-infected tomato.";
RL Plant Mol. Biol. 22:1017-1029(1993).
CC -!- FUNCTION: Defense against chitin-containing fungal pathogens.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide
CC (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14;
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space.
CC -!- INDUCTION: By fungal infection.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 19 family. Chitinase
CC class II subfamily. {ECO:0000305}.
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DR EMBL; Z15141; CAA78846.1; -; mRNA.
DR PIR; S37343; S37343.
DR RefSeq; NP_001234404.1; NM_001247475.2.
DR AlphaFoldDB; Q05539; -.
DR SMR; Q05539; -.
DR STRING; 4081.Solyc02g082920.2.1; -.
DR Allergome; 1273; Sola l Chitinase.
DR CAZy; GH19; Glycoside Hydrolase Family 19.
DR PaxDb; Q05539; -.
DR PRIDE; Q05539; -.
DR EnsemblPlants; Solyc02g082920.3.1; Solyc02g082920.3.1; Solyc02g082920.3.
DR GeneID; 544149; -.
DR Gramene; Solyc02g082920.3.1; Solyc02g082920.3.1; Solyc02g082920.3.
DR KEGG; sly:544149; -.
DR eggNOG; KOG4742; Eukaryota.
DR HOGENOM; CLU_045506_1_0_1; -.
DR InParanoid; Q05539; -.
DR OMA; PIQLTHQ; -.
DR OrthoDB; 1132954at2759; -.
DR PhylomeDB; Q05539; -.
DR Proteomes; UP000004994; Chromosome 2.
DR ExpressionAtlas; Q05539; baseline and differential.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR GO; GO:0004568; F:chitinase activity; IBA:GO_Central.
DR GO; GO:0016998; P:cell wall macromolecule catabolic process; IEA:InterPro.
DR GO; GO:0006032; P:chitin catabolic process; IEA:UniProtKB-KW.
DR GO; GO:0050832; P:defense response to fungus; IBA:GO_Central.
DR GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR016283; Glyco_hydro_19.
DR InterPro; IPR000726; Glyco_hydro_19_cat.
DR InterPro; IPR023346; Lysozyme-like_dom_sf.
DR Pfam; PF00182; Glyco_hydro_19; 1.
DR PIRSF; PIRSF001060; Endochitinase; 1.
DR SUPFAM; SSF53955; SSF53955; 1.
DR PROSITE; PS00773; CHITINASE_19_1; 1.
DR PROSITE; PS00774; CHITINASE_19_2; 1.
PE 1: Evidence at protein level;
KW Carbohydrate metabolism; Chitin degradation; Direct protein sequencing;
KW Disulfide bond; Glycosidase; Hydrolase; Pathogenesis-related protein;
KW Plant defense; Polysaccharide degradation; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000250"
FT CHAIN 25..253
FT /note="Acidic 26 kDa endochitinase"
FT /id="PRO_0000005299"
FT ACT_SITE 92
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:P29022"
FT DISULFID 212..244
FT /evidence="ECO:0000250"
SQ SEQUENCE 253 AA; 27632 MW; 6C78CA3750DE6D7A CRC64;
MKFNIVSPVA LSCLFFLFLT GTLAQNAGSI VTRELFEQML SFRNNDACPA KGFYTYDAFI
AAANSFPGFG TAGDDTARKK EIAAFFGQTS HETNGGSAGT FTGGYCFVKQ IEQSDRYYGR
GPIQLTHQSN YERAGQGIGV GQELVNNPDL VATDPIISFK TAIWFWMTEQ DNKPSCHNVI
IGQWTPSPKD TAANRVPGYG VITNIINGQF ECGMGPNTAA ESRIGFYRRY CGMLNVPTGE
NLDCNNQKNF AQG
