ID   CHIB_TOBAC              Reviewed;         294 AA.
AC   P29061;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=Basic endochitinase;
DE            EC=3.2.1.14;
DE   Flags: Precursor;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Xanthi; TISSUE=Leaf;
RX   PubMed=1643280; DOI=10.1007/bf00027070;
RA   Lawton K., Ward E., Payne G., Moyer M., Ryals J.;
RT   "Acidic and basic class III chitinase mRNA accumulation in response to TMV
RT   infection of tobacco.";
RL   Plant Mol. Biol. 19:735-743(1992).
CC   -!- FUNCTION: This protein functions as a defense against chitin containing
CC       fungal pathogens.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide
CC         (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14;
CC   -!- INDUCTION: By TMV infection.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 18 family. Chitinase
CC       class II subfamily. {ECO:0000305}.
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DR   EMBL; Z11564; CAA77657.1; -; mRNA.
DR   PIR; S23545; S23545.
DR   RefSeq; NP_001312735.1; NM_001325806.1.
DR   AlphaFoldDB; P29061; -.
DR   SMR; P29061; -.
DR   STRING; 4097.P29061; -.
DR   CAZy; GH18; Glycoside Hydrolase Family 18.
DR   GeneID; 107807601; -.
DR   KEGG; nta:107807601; -.
DR   OMA; RAGIKNC; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IBA:GO_Central.
DR   GO; GO:0004568; F:chitinase activity; IBA:GO_Central.
DR   GO; GO:0006032; P:chitin catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
DR   CDD; cd02877; GH18_hevamine_XipI_class_III; 1.
DR   InterPro; IPR045321; Cts1-like.
DR   InterPro; IPR001223; Glyco_hydro18_cat.
DR   InterPro; IPR001579; Glyco_hydro_18_chit_AS.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   Pfam; PF00704; Glyco_hydro_18; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   PROSITE; PS01095; GH18_1; 1.
DR   PROSITE; PS51910; GH18_2; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Chitin degradation; Disulfide bond; Glycosidase;
KW   Hydrolase; Polysaccharide degradation; Reference proteome; Signal.
FT   SIGNAL          1..24
FT   CHAIN           25..294
FT                   /note="Basic endochitinase"
FT                   /id="PRO_0000011919"
FT   DOMAIN          25..294
FT                   /note="GH18"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01258"
FT   ACT_SITE        151
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01258"
FT   DISULFID        44..91
FT                   /evidence="ECO:0000250"
FT   DISULFID        74..81
FT                   /evidence="ECO:0000250"
FT   DISULFID        182..211
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   294 AA;  32393 MW;  5CE49A1455C70AE1 CRC64;
     MNIKVSLLFI LPIFLLLLTS KVKAGDIVVY WGQDVGEGKL IDTCNSGLYN IVNIAFLSSF
     GNFQTPKLNL AGHCEPSSGG CQQLTKSIRH CQSIGIKIML SIGGGTPTYT LSSVDDARQV
     ADYLWNNFLG GQSSFRPLGD AVLDGIDFDI ELGQPHYIAL ARRLSEHGQQ GKKLYLTAAP
     QCPFPDKLLN GALQTGLFDY VWVQFYNNPE CEFMSNSENF KRRWNQWTSI PAKKLYIGLP
     AAKTAAGNGY IPKQVLMSQV LPFLKGSSKY GGVMLWNRKF DVQCGYSSAI RGAV