ID   CHIL1_HUMLU             Reviewed;         214 AA.
AC   A0A2U7XUH7;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   12-SEP-2018, sequence version 1.
DT   03-AUG-2022, entry version 12.
DE   RecName: Full=Chalcone isomerase-like protein 1 {ECO:0000303|PubMed:29760092};
DE            Short=HlCHIL1 {ECO:0000303|PubMed:29760092};
DE            EC=5.5.1.6 {ECO:0000250|UniProtKB:Q8VZW3};
GN   Name=CHIL1 {ECO:0000303|PubMed:29760092};
GN   Synonyms=HICHIL1 {ECO:0000303|PubMed:30468448};
OS   Humulus lupulus (European hop).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Rosales; Cannabaceae; Humulus.
OX   NCBI_TaxID=3486;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF VAL-139 AND ALA-143,
RP   PATHWAY, TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX   PubMed=29760092; DOI=10.1073/pnas.1802223115;
RA   Ban Z., Qin H., Mitchell A.J., Liu B., Zhang F., Weng J.-K., Dixon R.A.,
RA   Wang G.;
RT   "Noncatalytic chalcone isomerase-fold proteins in Humulus lupulus are
RT   auxiliary components in prenylated flavonoid biosynthesis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 115:E5223-E5232(2018).
RN   [2]
RP   REVIEW.
RX   PubMed=30468448; DOI=10.1039/c8np00077h;
RA   Liu Y., Jing S.-X., Luo S.-H., Li S.-H.;
RT   "Non-volatile natural products in plant glandular trichomes: chemistry,
RT   biological activities and biosynthesis.";
RL   Nat. Prod. Rep. 36:626-665(2019).
CC   -!- FUNCTION: Involved in the biosynthesis of prenylated phenolics natural
CC       products which contribute to the bitter taste of beer and display broad
CC       biological activities (Probable). Involved in anthocyanin biosynthesis
CC       (By similarity). Polyketide binding proteins (PBP) which reduces the
CC       catalytic activities of CHS_H1 and PT1L and prevents
CC       demethylxanthohumol (DMX) production, by binding to DMX and naringenin
CC       chalcone (NC) to stabilize the chalconoids ring-opened structure
CC       (PubMed:29760092). {ECO:0000250|UniProtKB:Q8VZW3,
CC       ECO:0000269|PubMed:29760092, ECO:0000305|PubMed:30468448}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a chalcone = a flavanone.; EC=5.5.1.6;
CC         Evidence={ECO:0000250|UniProtKB:Q8VZW3};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC       {ECO:0000269|PubMed:29760092}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:29760092}.
CC   -!- TISSUE SPECIFICITY: Mostly expressed in glandular trichomes (lupulin
CC       glands), and, to a lower extent, in cones, cones bracts, leaves, stems
CC       and roots. {ECO:0000269|PubMed:29760092}.
CC   -!- SIMILARITY: Belongs to the chalcone isomerase family. {ECO:0000305}.
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DR   EMBL; MG324004; AVR53896.1; -; mRNA.
DR   AlphaFoldDB; A0A2U7XUH7; -.
DR   SMR; A0A2U7XUH7; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR   GO; GO:0045430; F:chalcone isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.890.20; -; 1.
DR   Gene3D; 3.50.70.10; -; 1.
DR   InterPro; IPR016088; Chalcone_isomerase_3-sand.
DR   InterPro; IPR016089; Chalcone_isomerase_bundle_sf.
DR   InterPro; IPR036298; Chalcone_isomerase_sf.
DR   InterPro; IPR044228; FAP1.
DR   PANTHER; PTHR47589; PTHR47589; 1.
DR   SUPFAM; SSF54626; SSF54626; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Flavonoid biosynthesis; Isomerase.
FT   CHAIN           1..214
FT                   /note="Chalcone isomerase-like protein 1"
FT                   /id="PRO_0000452944"
FT   SITE            139
FT                   /note="Involved in polyketide binding"
FT                   /evidence="ECO:0000305|PubMed:29760092"
FT   SITE            143
FT                   /note="Involved in polyketide binding"
FT                   /evidence="ECO:0000305|PubMed:29760092"
FT   MUTAGEN         139
FT                   /note="V->F: Higher affinity for demethylxanthohumol (DMX)
FT                   and naringenin chalcone (NC). Reduced stabilization by DMX
FT                   and NC; when associated with F-143."
FT                   /evidence="ECO:0000269|PubMed:29760092"
FT   MUTAGEN         143
FT                   /note="A->F: Reduced stabilization by demethylxanthohumol
FT                   (DMX) and naringenin chalcone (NC); when associated with F-
FT                   139."
FT                   /evidence="ECO:0000269|PubMed:29760092"
SQ   SEQUENCE   214 AA;  23377 MW;  7A1850310A4A4816 CRC64;
     MATALNSKNA SSNTAVHIEP KTGIAFPVKL DDGKSLNSVG LRKKSLLGMG IKVFGFGLYA
     DNEKLKNLLK LKIGKSPAKP TEEMYQLVID GDIGLTHKIV IAYSGLKMNM FKKAFSEALG
     ESIMKLNGGR KNEELANKVL GPASDQIKLA TGSEMEISKL PGYVLETKVH GELASRVESE
     LLCRAYFGIY LGEITMECYK ESKEMFGQSM LSLF