ID   CHIL2_HUMLU             Reviewed;         209 AA.
AC   A0A2U7XUE3;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   12-SEP-2018, sequence version 1.
DT   03-AUG-2022, entry version 14.
DE   RecName: Full=Chalcone isomerase-like protein 2 {ECO:0000303|PubMed:29760092};
DE            Short=HlCHIL2 {ECO:0000303|PubMed:29760092};
DE            EC=5.5.1.6 {ECO:0000250|UniProtKB:Q8VZW3};
GN   Name=CHIL2 {ECO:0000303|PubMed:29760092};
GN   Synonyms=HICHIL2 {ECO:0000303|PubMed:30468448};
OS   Humulus lupulus (European hop).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Rosales; Cannabaceae; Humulus.
OX   NCBI_TaxID=3486;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, PATHWAY, TISSUE SPECIFICITY,
RP   SUBCELLULAR LOCATION, AND INTERACTION WITH CHS_H1 AND PT1L.
RX   PubMed=29760092; DOI=10.1073/pnas.1802223115;
RA   Ban Z., Qin H., Mitchell A.J., Liu B., Zhang F., Weng J.-K., Dixon R.A.,
RA   Wang G.;
RT   "Noncatalytic chalcone isomerase-fold proteins in Humulus lupulus are
RT   auxiliary components in prenylated flavonoid biosynthesis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 115:E5223-E5232(2018).
RN   [2]
RP   REVIEW.
RX   PubMed=30468448; DOI=10.1039/c8np00077h;
RA   Liu Y., Jing S.-X., Luo S.-H., Li S.-H.;
RT   "Non-volatile natural products in plant glandular trichomes: chemistry,
RT   biological activities and biosynthesis.";
RL   Nat. Prod. Rep. 36:626-665(2019).
CC   -!- FUNCTION: Involved in the biosynthesis of prenylated phenolics natural
CC       products which contribute to the bitter taste of beer and display broad
CC       biological activities (Probable). Involved in anthocyanin biosynthesis
CC       (By similarity). Polyketide binding proteins (PBP) which promotes the
CC       catalytic activities of CHS_H1 and PT1L and triggers
CC       demethylxanthohumol (DMX) production (PubMed:29760092).
CC       {ECO:0000250|UniProtKB:Q8VZW3, ECO:0000269|PubMed:29760092,
CC       ECO:0000305|PubMed:30468448}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a chalcone = a flavanone.; EC=5.5.1.6;
CC         Evidence={ECO:0000250|UniProtKB:Q8VZW3};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC       {ECO:0000269|PubMed:29760092}.
CC   -!- SUBUNIT: Component an active demethylxanthohumol (DMX) biosynthetic
CC       metabolon in glandular trichomes (lupulin glands) that encompasses a
CC       chalcone synthase (CHS) and a membrane-bound prenyltransferase
CC       (PubMed:29760092). Interacts with CHS_H1 and PT1L (PubMed:29760092).
CC       {ECO:0000269|PubMed:29760092}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:29760092}.
CC   -!- TISSUE SPECIFICITY: Mostly expressed in glandular trichomes (lupulin
CC       glands), and, to a lower extent, in cones, cones bracts, leaves, stems
CC       and roots. {ECO:0000269|PubMed:29760092}.
CC   -!- SIMILARITY: Belongs to the chalcone isomerase family. {ECO:0000305}.
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DR   EMBL; MG324005; AVR53897.1; -; mRNA.
DR   AlphaFoldDB; A0A2U7XUE3; -.
DR   SMR; A0A2U7XUE3; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR   GO; GO:0045430; F:chalcone isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.890.20; -; 1.
DR   Gene3D; 3.50.70.10; -; 1.
DR   InterPro; IPR016087; Chalcone_isomerase.
DR   InterPro; IPR016088; Chalcone_isomerase_3-sand.
DR   InterPro; IPR016089; Chalcone_isomerase_bundle_sf.
DR   InterPro; IPR036298; Chalcone_isomerase_sf.
DR   InterPro; IPR044191; CHI3-like.
DR   PANTHER; PTHR47588; PTHR47588; 1.
DR   Pfam; PF02431; Chalcone; 1.
DR   SUPFAM; SSF54626; SSF54626; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Flavonoid biosynthesis; Isomerase.
FT   CHAIN           1..209
FT                   /note="Chalcone isomerase-like protein 2"
FT                   /id="PRO_0000452945"
SQ   SEQUENCE   209 AA;  23552 MW;  5A05F9D6D0241154 CRC64;
     MENNMVMVHE IPFPPEIKTT KPLSLLGYGI TDMEIHFLQV KFTAIGVYLD SDVVKHLQQW
     KGKKGNELAE DDDFFDALIS APVEKYLRIV VIKEIKGSQY GVQLESSVRD RLAAEDMYEE
     EEEAALEKIV EFLQSKYFKK DTLITFHFPA TSPTAEIVVT LEGKEESKLK VENKNVVDMI
     KKWYLGGTSG VSPSTISSLA DNLSAELSK