CHIP_ECOLI
ID CHIP_ECOLI Reviewed; 468 AA.
AC P75733;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Chitoporin;
DE AltName: Full=ChiP-III;
DE Flags: Precursor;
GN Name=chiP; Synonyms=ybfM; OrderedLocusNames=b0681, JW0667;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP SUBCELLULAR LOCATION, AND SIMILARITY.
RX PubMed=12192075; DOI=10.1110/ps.0209002;
RA Zhai Y., Saier M.H. Jr.;
RT "The beta-barrel finder (BBF) program, allowing identification of outer
RT membrane beta-barrel proteins encoded within prokaryotic genomes.";
RL Protein Sci. 11:2196-2207(2002).
RN [5]
RP FUNCTION, AND GENE NAME.
RX PubMed=16857666; DOI=10.1074/jbc.m605052200;
RA Yang C., Rodionov D.A., Li X., Laikova O.N., Gelfand M.S., Zagnitko O.P.,
RA Romine M.F., Obraztsova A.Y., Nealson K.H., Osterman A.L.;
RT "Comparative genomics and experimental characterization of N-
RT acetylglucosamine utilization pathway of Shewanella oneidensis.";
RL J. Biol. Chem. 281:29872-29885(2006).
RN [6]
RP INDUCTION.
RC STRAIN=K12;
RX PubMed=19400782; DOI=10.1111/j.1365-2958.2009.06688.x;
RA Rasmussen A.A., Johansen J., Nielsen J.S., Overgaard M., Kallipolitis B.,
RA Valentin-Hansen P.;
RT "A conserved small RNA promotes silencing of the outer membrane protein
RT YbfM.";
RL Mol. Microbiol. 72:566-577(2009).
RN [7]
RP FUNCTION IN CHITOBIOSE UTILIZATION, AND INDUCTION.
RC STRAIN=K12;
RX PubMed=19682266; DOI=10.1111/j.1365-2958.2009.06807.x;
RA Overgaard M., Johansen J., Moller-Jensen J., Valentin-Hansen P.;
RT "Switching off small RNA regulation with trap-mRNA.";
RL Mol. Microbiol. 73:790-800(2009).
CC -!- FUNCTION: Involved in the uptake of chitosugars.
CC {ECO:0000305|PubMed:16857666, ECO:0000305|PubMed:19682266}.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane
CC {ECO:0000305|PubMed:12192075}; Multi-pass membrane protein
CC {ECO:0000305|PubMed:12192075}.
CC -!- INDUCTION: In the absence of chitobiose, expression of chiPQ is
CC silenced by the MicM small regulatory RNA (sRNA), which sequesters the
CC ribosome binding site of the chiPQ mRNA by an antisense mechanism. In
CC the presence of chitosugars, the chbBCARFG chitobiose operon is induced
CC and acts as an RNA trap to degrade the constitutively expressed MicM,
CC leading to the translation of chiPQ. {ECO:0000269|PubMed:19400782,
CC ECO:0000269|PubMed:19682266}.
CC -!- SIMILARITY: Belongs to the outer membrane porin (Opr) (TC 1.B.25)
CC family. {ECO:0000305}.
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DR EMBL; U00096; AAC73775.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35329.2; -; Genomic_DNA.
DR PIR; H64802; H64802.
DR RefSeq; NP_415207.1; NC_000913.3.
DR RefSeq; WP_001258819.1; NZ_SSZK01000045.1.
DR AlphaFoldDB; P75733; -.
DR SMR; P75733; -.
DR BioGRID; 4261649; 315.
DR STRING; 511145.b0681; -.
DR TCDB; 1.B.25.1.13; the outer membrane porin (opr) family.
DR PaxDb; P75733; -.
DR PRIDE; P75733; -.
DR EnsemblBacteria; AAC73775; AAC73775; b0681.
DR EnsemblBacteria; BAA35329; BAA35329; BAA35329.
DR GeneID; 945296; -.
DR KEGG; ecj:JW0667; -.
DR KEGG; eco:b0681; -.
DR PATRIC; fig|1411691.4.peg.1595; -.
DR EchoBASE; EB3423; -.
DR eggNOG; ENOG502Z7J2; Bacteria.
DR HOGENOM; CLU_045968_0_0_6; -.
DR InParanoid; P75733; -.
DR OMA; TWVKAEG; -.
DR PhylomeDB; P75733; -.
DR BioCyc; EcoCyc:G6370-MON; -.
DR BioCyc; MetaCyc:G6370-MON; -.
DR PRO; PR:P75733; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0046930; C:pore complex; IEA:UniProtKB-KW.
DR GO; GO:0015267; F:channel activity; IDA:EcoCyc.
DR GO; GO:0015288; F:porin activity; IBA:GO_Central.
DR GO; GO:0052778; P:diacetylchitobiose metabolic process; IEP:EcoCyc.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR GO; GO:0015772; P:oligosaccharide transport; IDA:EcoCyc.
DR GO; GO:0015774; P:polysaccharide transport; IEA:UniProtKB-KW.
DR Gene3D; 2.40.160.10; -; 1.
DR InterPro; IPR005318; OM_porin_bac.
DR InterPro; IPR023614; Porin_dom_sf.
DR PANTHER; PTHR34596; PTHR34596; 1.
DR Pfam; PF03573; OprD; 1.
PE 1: Evidence at protein level;
KW Cell outer membrane; Ion transport; Membrane; Polysaccharide transport;
KW Porin; Reference proteome; Signal; Sugar transport; Transmembrane;
KW Transmembrane beta strand; Transport.
FT SIGNAL 1..32
FT /evidence="ECO:0000255"
FT CHAIN 33..468
FT /note="Chitoporin"
FT /id="PRO_0000168697"
SQ SEQUENCE 468 AA; 52780 MW; 954B5A778AE1C2E4 CRC64;
MRTFSGKRST LALAIAGVTA MSGFMAMPEA RAEGFIDDST LTGGIYYWQR ERDRKDVTDG
DKYKTNLSHS TWNANLDFQS GYAADMFGLD IAAFTAIEMA ENGDSSHPNE IAFSKSNKAY
DEDWSGDKSG ISLYKAAAKF KYGPVWARAG YIQPTGQTLL APHWSFMPGT YQGAEAGANF
DYGDAGALSF SYMWTNEYKA PWHLEMDEFY QNDKTTKVDY LHSFGAKYDF KNNFVLEAAF
GQAEGYIDQY FAKASYKFDI AGSPLTTSYQ FYGTRDKVDD RSVNDLYDGT AWLQALTFGY
RAADVVDLRL EGTWVKADGQ QGYFLQRMTP TYASSNGRLD IWWDNRSDFN ANGEKAVFFG
AMYDLKNWNL PGFAIGASYV YAWDAKPATW QSNPDAYYDK NRTIEESAYS LDAVYTIQDG
RAKGTMFKLH FTEYDNHSDI PSWGGGYGNI FQDERDVKFM VIAPFTIF
