CHIQ_ECOLI
ID CHIQ_ECOLI Reviewed; 108 AA.
AC P75734;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Uncharacterized lipoprotein ChiQ;
DE Flags: Precursor;
GN Name=chiQ; Synonyms=ybfN; OrderedLocusNames=b0682, JW0668;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=12876315; DOI=10.1110/ps.0303703;
RA Juncker A.S., Willenbrock H., Von Heijne G., Brunak S., Nielsen H.,
RA Krogh A.;
RT "Prediction of lipoprotein signal peptides in Gram-negative bacteria.";
RL Protein Sci. 12:1652-1662(2003).
RN [5]
RP INDUCTION.
RC STRAIN=K12;
RX PubMed=19400782; DOI=10.1111/j.1365-2958.2009.06688.x;
RA Rasmussen A.A., Johansen J., Nielsen J.S., Overgaard M., Kallipolitis B.,
RA Valentin-Hansen P.;
RT "A conserved small RNA promotes silencing of the outer membrane protein
RT YbfM.";
RL Mol. Microbiol. 72:566-577(2009).
RN [6]
RP INDUCTION.
RC STRAIN=K12;
RX PubMed=19682266; DOI=10.1111/j.1365-2958.2009.06807.x;
RA Overgaard M., Johansen J., Moller-Jensen J., Valentin-Hansen P.;
RT "Switching off small RNA regulation with trap-mRNA.";
RL Mol. Microbiol. 73:790-800(2009).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:12876315};
CC Lipid-anchor {ECO:0000305|PubMed:12876315}.
CC -!- INDUCTION: In the absence of chitobiose, expression of chiPQ is
CC silenced by the MicM small regulatory RNA (sRNA), which sequesters the
CC ribosome binding site of the chiPQ mRNA by an antisense mechanism. In
CC the presence of chitosugars, the chbBCARFG chitobiose operon is induced
CC and acts as an RNA trap to degrade the constitutively expressed MicM,
CC leading to the translation of chiPQ. {ECO:0000269|PubMed:19400782,
CC ECO:0000269|PubMed:19682266}.
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DR EMBL; U00096; AAC73776.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35330.1; -; Genomic_DNA.
DR PIR; A64803; A64803.
DR RefSeq; NP_415208.1; NC_000913.3.
DR RefSeq; WP_000733579.1; NZ_SSZK01000045.1.
DR AlphaFoldDB; P75734; -.
DR BioGRID; 4261791; 158.
DR STRING; 511145.b0682; -.
DR PaxDb; P75734; -.
DR PRIDE; P75734; -.
DR EnsemblBacteria; AAC73776; AAC73776; b0682.
DR EnsemblBacteria; BAA35330; BAA35330; BAA35330.
DR GeneID; 66671048; -.
DR GeneID; 945713; -.
DR KEGG; ecj:JW0668; -.
DR KEGG; eco:b0682; -.
DR PATRIC; fig|511145.12.peg.711; -.
DR EchoBASE; EB3424; -.
DR eggNOG; ENOG5031F99; Bacteria.
DR HOGENOM; CLU_2192888_0_0_6; -.
DR OMA; QFAEQET; -.
DR PhylomeDB; P75734; -.
DR BioCyc; EcoCyc:G6371-MON; -.
DR PRO; PR:P75734; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR025727; YbfN-like.
DR Pfam; PF13982; YbfN; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Lipoprotein; Membrane; Palmitate; Reference proteome;
KW Signal.
FT SIGNAL 1..16
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 17..108
FT /note="Uncharacterized lipoprotein ChiQ"
FT /id="PRO_0000018039"
FT LIPID 17
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 17
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ SEQUENCE 108 AA; 11999 MW; 657B980289D18AC9 CRC64;
MKKLILIAIM ASGLVACAQS TAPQEDSRLK EAYSACINTA QGSPEKIEAC QSVLNVLKKE
KQHQQFADQE SVRVLDYQQC LRATQTGNDQ AVKADCDKVW QEIRSNNK
