ID   CHIS_NOCSP              Reviewed;         278 AA.
AC   P48846;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Chitosanase;
DE            EC=3.2.1.132;
DE   Flags: Precursor;
GN   Name=csn;
OS   Nocardioides sp. (strain N106).
OC   Bacteria; Actinobacteria; Propionibacteriales; Nocardioidaceae;
OC   Nocardioides.
OX   NCBI_TaxID=47919;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7582023; DOI=10.1099/13500872-141-10-2629;
RA   Masson J.Y., Boucher I., Neugebauer W.A., Ramotar D., Brzezinski R.;
RT   "A new chitosanase gene from a Nocardioides sp. is a third member of
RT   glycosyl hydrolase family 46.";
RL   Microbiology 141:2629-2635(1995).
CC   -!- FUNCTION: Aids in the defense against invading fungal pathogens by
CC       degrading their cell wall chitosan.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of beta-(1->4)-linkages between D-glucosamine
CC         residues in a partly acetylated chitosan.; EC=3.2.1.132;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 46 family. {ECO:0000305}.
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DR   EMBL; L40408; AAA63405.1; -; Genomic_DNA.
DR   AlphaFoldDB; P48846; -.
DR   SMR; P48846; -.
DR   CAZy; GH46; Glycoside Hydrolase Family 46.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016977; F:chitosanase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd00978; chitosanase_GH46; 1.
DR   Gene3D; 3.30.386.10; -; 1.
DR   InterPro; IPR000400; Glyco_hydro_46.
DR   InterPro; IPR023099; Glyco_hydro_46_N.
DR   InterPro; IPR023346; Lysozyme-like_dom_sf.
DR   Pfam; PF01374; Glyco_hydro_46; 1.
DR   PIRSF; PIRSF036551; Chitosanase; 1.
DR   SUPFAM; SSF53955; SSF53955; 1.
DR   PROSITE; PS60000; CHITOSANASE_46_80; 1.
PE   3: Inferred from homology;
KW   Glycosidase; Hydrolase; Secreted; Signal.
FT   SIGNAL          1..41
FT                   /evidence="ECO:0000255"
FT   CHAIN           42..278
FT                   /note="Chitosanase"
FT                   /id="PRO_0000012208"
FT   ACT_SITE        63
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10070"
FT   ACT_SITE        81
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   278 AA;  29987 MW;  E76AA20065FAB4FF CRC64;
     MRLKHPTARL ALAALLVAVP RSVAAAGTVH AAPAPAGATR LAAVGLDDPH KKDIAMQLVS
     SAENSSLDWK SQYKYIEDIK DGRGYTAGII GFCSGTGDML DLVADYTDLK PGNILAKYLP
     ALRKVNGTES HAGLASAFEK DWATAAKDSV FQQAQNDERD RSYFNPAVNQ AKASLRALGQ
     FAYYDAIVMH GPGDSSDSFG GIRKAAMKKA KTPAQGRDEA TYLKAFLAAR KTVMLKEEAH
     SDTSRVDTEQ TVFLNAKNFD LNPPLKWKVY GDSYAINS